Nuclear myosin I mediates genomic clustering of estrogen receptor-α without affecting ligand-induced transcriptional robustness DOI Creative Commons

Sudha Swaminathan,

Ishfaq Ahamd Pandith,

Rajat Mann

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Abstract Ligand-induced transcriptional responses are rapid and robust. Estrogen, one such ligand, exerts its effects through the steroid hormone receptor estrogen receptor-alpha (ERα). Upon stimulation, ERα binds to clustered enhancers drive target gene expression. Here, we identify nuclear myosin 1 (NM1/Myo1c) as a positive regulator of clustering on enhancers, promoting condensate formation chromatin. NM1 is enriched chromatin at peak signaling response, depletion leads genome-wide reduction in occupancy condensates. Surprisingly, these alterations have minimal impact estrogen-regulated expression, suggesting that output remains robust despite disruptions transcription factor clustering. This study reveals regulatory axis between Myo1c clustering, offering fresh insights into intricacies regulation.

Language: Английский

Nuclear myosin I mediates genomic clustering of estrogen receptor-α without affecting ligand-induced transcriptional robustness DOI Creative Commons

Sudha Swaminathan,

Ishfaq Ahamd Pandith,

Rajat Mann

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Abstract Ligand-induced transcriptional responses are rapid and robust. Estrogen, one such ligand, exerts its effects through the steroid hormone receptor estrogen receptor-alpha (ERα). Upon stimulation, ERα binds to clustered enhancers drive target gene expression. Here, we identify nuclear myosin 1 (NM1/Myo1c) as a positive regulator of clustering on enhancers, promoting condensate formation chromatin. NM1 is enriched chromatin at peak signaling response, depletion leads genome-wide reduction in occupancy condensates. Surprisingly, these alterations have minimal impact estrogen-regulated expression, suggesting that output remains robust despite disruptions transcription factor clustering. This study reveals regulatory axis between Myo1c clustering, offering fresh insights into intricacies regulation.

Language: Английский

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