Neuronal
ensembles,
coactive
groups
of
neurons
found
in
spontaneous
and
evoked
cortical
activity,
are
causally
related
to
memories
perception,
but
it
is
still
unknown
how
stable
or
flexible
they
over
time.
We
used
two-photon
multiplane
calcium
imaging
track
weeks
the
activity
same
pyramidal
layer
2/3
visual
cortex
from
awake
mice
recorded
their
visually
responses.
Less
than
half
remained
active
across
any
two
sessions.
These
formed
ensembles
that
lasted
weeks,
some
were
also
transient
appeared
only
one
single
session.
Stable
preserved
most
for
up
46
days,
our
longest
imaged
period,
these
'core'
cells
had
stronger
functional
connectivity.
Our
results
demonstrate
neuronal
can
last
could,
principle,
serve
as
a
substrate
long-lasting
representation
perceptual
states
memories.
Journal of Optics,
Journal Year:
2020,
Volume and Issue:
22(12), P. 123002 - 123002
Published: Aug. 28, 2020
Contents1.
Materials
for
dynamic
holographic
3D
display
2.
Photopolymerizable
nanocomposite
materials
applications
3.
Photopolymers
the
recording
of
waveguides
4.
Shrinkage
in
materials:
photopolymers
5.
Advances
photorefractive
and
devices
6.
Industrial-scale
material
mass
production
vHOEs
7.
Non-imaging
optics
solar
energy
conversion
8.
Data
security
data
storage
9.
Multimodal
acquisition
using
digital
holography
10.EUV
SXR
tomography
with
compact
short
wavelength
sources
11.Holographic
wavefront
sensors
12.
Holographic
microscopy
goes
incoherent
13.Digital
polarized
life
science
14.Using
PA-LCoS
microdisplays
15.Playing
piano
brain:
imaging
manipulation
neural
activity
16.Holography
astronomical
spectrographs
17.Digital
based
on
spatial
coherence
function
18.
Autofocus
19.Interference
lithography
nanostructures
fabrication
20.Applications
deep
learning
21.Digital
focusing
laser
speckle
contrast
22.
Ultrafast
spatio-temporal
metrology
23.From
a
conventional
to
wide-sense
24.Building
functional
waveguide
microstructures
nonlinear
waves
light
References
Neuron,
Journal Year:
2022,
Volume and Issue:
110(7), P. 1139 - 1155.e6
Published: Feb. 3, 2022
The
biophysical
properties
of
existing
optogenetic
tools
constrain
the
scale,
speed,
and
fidelity
precise
control.
Here,
we
use
structure-guided
mutagenesis
to
engineer
opsins
that
exhibit
very
high
potency
while
retaining
fast
kinetics.
These
new
enable
large-scale,
temporally
spatially
control
population
neural
activity.
We
extensively
benchmark
these
against
provide
a
detailed
characterization
diverse
family
under
two-photon
illumination.
This
establishes
resource
for
matching
optimal
opsin
goals
constraints
patterned
optogenetics
experiments.
Finally,
by
combining
with
optimized
procedures
holographic
photostimulation,
demonstrate
simultaneous
coactivation
several
hundred
defined
neurons
single
hologram
nearly
double
number
interleaving
holograms
at
rates.
newly
engineered
substantially
extend
capabilities
illumination
paradigms
addressing
circuits
behavior.
Neuronal
ensembles,
coactive
groups
of
neurons
found
in
spontaneous
and
evoked
cortical
activity,
are
causally
related
to
memories
perception,
but
it
is
still
unknown
how
stable
or
flexible
they
over
time.
We
used
two-photon
multiplane
calcium
imaging
track
weeks
the
activity
same
pyramidal
layer
2/3
visual
cortex
from
awake
mice
recorded
their
visually
responses.
Less
than
half
remained
active
across
any
two
sessions.
These
formed
ensembles
that
lasted
weeks,
some
were
also
transient
appeared
only
one
single
session.
Stable
preserved
most
for
up
46
days,
our
longest
imaged
period,
these
'core'
cells
had
stronger
functional
connectivity.
Our
results
demonstrate
neuronal
can
last
could,
principle,
serve
as
a
substrate
long-lasting
representation
perceptual
states
memories.
