Yeast metabolic innovations emerged via expanded metabolic network and gene positive selection

Molecular Systems Biology, Journal Year: 2021, Volume and Issue: 17(10)

Published: Oct. 1, 2021

Yeasts are known to have versatile metabolic traits, while how these traits evolved has not been elucidated systematically. We performed integrative evolution analysis investigate genomic determines trait generation by reconstructing genome-scale models (GEMs) for 332 yeasts. These GEMs could comprehensively characterize diversity and predict enzyme functionality, thereby signifying that sequence-level shaped reaction networks towards new functions. Strikingly, using GEMs, we can mechanistically map different evolutionary events, e.g. horizontal gene transfer duplication, onto relevant subpathways explain plasticity. This demonstrates family expansion promiscuity prominent mechanisms gains, GEM simulations reveal additional factors, such as loss from distant pathways, contribute losses. Furthermore, our pinpoint specific genes pathways under positive selection the formulation of complex i.e. thermotolerance Crabtree effect. Our findings illustrate multidimensional in both network structure individual enzymes drives phenotypic variations.

Language: Английский

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Yeasts from tropical forests: Biodiversity, ecological interactions, and as sources of bioinnovation

Yeast, Journal Year: 2023, Volume and Issue: 40(11), P. 511 - 539

Published: Nov. 1, 2023

Abstract Tropical rainforests and related biomes are found in Asia, Australia, Africa, Central South America, Mexico, many Pacific Islands. These encompass less than 20% of Earth's terrestrial area, may contain about 50% the planet's biodiversity, endangered regions vulnerable to deforestation. have a great diversity substrates that can be colonized by yeasts. unicellular fungi contribute recycling organic matter, serve as food source for other organisms, or ecological interactions benefit harm plants, animals, fungi. In this review, we summarize most important studies yeast biodiversity carried out these biomes, well new data, discuss ecology genera frequently isolated from tropical forests potential microorganisms bioinnovation. We show forest represent tremendous species. Although studies, using culture‐dependent methods, already been Africa Australasia remain an underexplored novel hope review will encourage researchers study yeasts unexplored habitats.

Language: Английский

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Microbial Dynamics in Sour–Sweet Wine Vinegar: Impacts on Chemical and Sensory Composition

Applied Sciences, Journal Year: 2023, Volume and Issue: 13(13), P. 7366 - 7366

Published: June 21, 2023

The most known and traditional vinegar is the one that made from wine. For its production, grape must undergo alcohol fermentation posterior oxidation of ethanol to acetic acid. Yeasts acid bacteria (AAB) carry out biochemical processes in sequence. process wine acetification can be achieved by slow (the Orléans or French methods) a quick submerged industrial process. High-quality usually produced methods using oak casks, once wood allows continuous aeration culture. Sour–sweet presents balance both bitter/sour sweet flavors. sourness typically comes acid, while sweetness come type fruit amount sugar present at end production. In general, sour-sweet has more complex nuanced flavor profile compared regular vinegar, which often simply sour. One kind where yeasts co-exist produce savory balsamic (TBV), Italy. Spain, fortified sherry also been used for Portugal, some companies have port since 2018. These three distinctive products common found, well balanced with sourness. this review, we analyze sour-sweat process, bearing mind possible interactions between AAB occur during production considering symbiotic competitive features diverse microbiota.

Language: Английский

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Horizontal gene transfer in yeasts

Current Opinion in Genetics & Development, Journal Year: 2022, Volume and Issue: 76, P. 101950 - 101950

Published: July 13, 2022

Language: Английский

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Multilayered horizontal operon transfers from bacteria reconstruct a thiamine salvage pathway in yeasts

Proceedings of the National Academy of Sciences, Journal Year: 2019, Volume and Issue: 116(44), P. 22219 - 22228

Published: Oct. 14, 2019

Horizontal acquisition of bacterial genes is presently recognized as an important contribution to the adaptation and evolution eukaryotic genomes. However, mechanisms underlying expression consequent selection fixation prokaryotic in new setting are largely unknown. Here we show that composing pathway for synthesis essential vitamin B1 (thiamine) were lost ancestor a yeast lineage, Wickerhamiella/Starmerella (W/S) clade, known harbor unusually large number alien origin. The thiamine was subsequently reassembled, at least twice, by multiple HGT events from different donors involving both single entire operons. In W/S-clade species Starmerella bombicola obtained direct genetic evidence all functional. reconstructed composed operating coordinately scavenge derivatives environment. newly acquired operons involved repertoire until now only sparsely documented, namely longer intergenic regions, post-horizontal gene transfer (HGT) fusions fostering coordinated expression, relocation, possibly recombination generating mosaic genes. results provide additional occurred recurrently this lineage crucial reestablishment functions similar used across broad range microbes promote their

Language: Английский

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