Spatiotemporal constraints on optogenetic inactivation in cortical circuits

eLife, Journal Year: 2019, Volume and Issue: 8

Published: Nov. 18, 2019

Optogenetics allows manipulations of genetically and spatially defined neuronal populations with excellent temporal control. However, neurons are coupled other over multiple length scales, the effects localized thus spread beyond targeted neurons. We benchmarked several optogenetic methods to inactivate small regions neocortex. Optogenetic excitation GABAergic produced more effective inactivation than light-gated ion pumps. Transgenic mice expressing light-dependent chloride channel GtACR1 most potent inactivation. Generally, substantially photostimulation light, caused by strong coupling between cortical Over some range light intensity, inhibitory reduced activity in these neurons, together pyramidal a signature inhibition-stabilized neural networks ('paradoxical effect'). The offset was followed rebound dose-dependent manner, limiting resolution. Our data offer guidance for design vivo optogenetics experiments.

Language: Английский

---

Inhibitory stabilization and cortical computation

Nature reviews. Neuroscience, Journal Year: 2020, Volume and Issue: 22(1), P. 21 - 37

Published: Nov. 11, 2020

Language: Английский

---

An increase of inhibition drives the developmental decorrelation of neural activity

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Aug. 17, 2022

Throughout development, the brain transits from early highly synchronous activity patterns to a mature state with sparse and decorrelated neural activity, yet mechanisms underlying this process are poorly understood. The developmental transition has important functional consequences, as latter is thought allow for more efficient storage, retrieval, processing of information. Here, we show that, in mouse medial prefrontal cortex (mPFC), during first two postnatal weeks decorrelates following specific spatial patterns. This accompanied by concomitant tilting excitation-inhibition (E-I) ratio toward inhibition. Using optogenetic manipulations network modeling, that phenomena mechanistically linked, relative increase inhibition drives decorrelation activity. Accordingly, mice mimicking etiology neurodevelopmental disorders, subtle alterations E-I associated impairments correlational structure spike trains. Finally, capitalizing on EEG data newborn babies, an analogous takes place also human brain. Thus, changes control (de)correlation and, these means, its imbalance might contribute pathogenesis disorders.

Language: Английский

---

What is the dynamical regime of cerebral cortex?

Neuron, Journal Year: 2021, Volume and Issue: 109(21), P. 3373 - 3391

Published: Aug. 30, 2021

Language: Английский

---

Optimal anticipatory control as a theory of motor preparation: A thalamo-cortical circuit model

Neuron, Journal Year: 2021, Volume and Issue: 109(9), P. 1567 - 1581.e12

Published: March 30, 2021

Across a range of motor and cognitive tasks, cortical activity can be accurately described by low-dimensional dynamics unfolding from specific initial conditions on every trial. These "preparatory states" largely determine the subsequent evolution both neural behavior, their importance raises questions regarding how they are, or ought to be, set. Here, we formulate preparation as optimal anticipatory control future movements show that solution requires form internal feedback circuit dynamics. In contrast simple feedforward strategy, enables fast movement selectively controlling state in small subspace matters for upcoming movement. Feedback but not explains orthogonality between preparatory observed reaching monkeys. We propose model which is implemented thalamo-cortical loop gated basal ganglia.

Language: Английский

---

Lateral inhibition in V1 controls neural and perceptual contrast sensitivity

Nature Neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Language: Английский

---

A Disinhibitory Circuit for Contextual Modulation in Primary Visual Cortex

Neuron, Journal Year: 2020, Volume and Issue: 108(6), P. 1181 - 1193.e8

Published: Dec. 1, 2020

Language: Английский

---

How many neurons are sufficient for perception of cortical activity?

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Oct. 26, 2020

Many theories of brain function propose that activity in sparse subsets neurons underlies perception and action. To place a lower bound on the amount neural can be perceived, we used an all-optical approach to drive behaviour with targeted two-photon optogenetic activation small ensembles L2/3 pyramidal mouse barrel cortex while simultaneously recording local network calcium imaging. By precisely titrating number stimulated, demonstrate for cortical is ~14 neurons. We find steep sigmoidal relationship between activated behaviour, saturating at only ~37 neurons, show this shift learning. Furthermore, balanced by inhibition neighbouring This surprising perceptual sensitivity face potent suppression supports coding hypothesis, suggests balances trade-off minimizing impact noise efficiently detecting relevant signals.

Language: Английский

---

VIP interneurons in mouse primary visual cortex selectively enhance responses to weak but specific stimuli

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Oct. 27, 2020

Vasoactive intestinal peptide-expressing (VIP) interneurons in the cortex regulate feedback inhibition of pyramidal neurons through suppression somatostatin-expressing (SST) and, reciprocally, SST inhibit VIP neurons. Although neuron activity primary visual (V1) mouse is highly correlated with locomotion, relevance locomotion-related to coding not known. Here we show that V1 respond strongly low contrast front-to-back motion congruent self-motion during locomotion but are suppressed by other directions and contrasts. have complementary tuning. Layer 2/3 contains a substantially larger population preferring than deeper layers, layer (but layer) bias for specifically at contrast. Network modeling indicates VIP-SST mutual antagonism regulates gain achieve sensitivity specific weak stimuli without compromising network stability.

Language: Английский

---

Regulation of circuit organization and function through inhibitory synaptic plasticity

Trends in Neurosciences, Journal Year: 2022, Volume and Issue: 45(12), P. 884 - 898

Published: Oct. 28, 2022

Language: Английский

---