Sequential events during the quiescence to proliferation transition establish patterns of follicle cell differentiation in the Drosophila ovary DOI Creative Commons
Eric H. Lee, Daniel Zinshteyn,

Fred Miglo

et al.

Biology Open, Journal Year: 2022, Volume and Issue: 12(1)

Published: Dec. 16, 2022

Stem cells cycle between periods of quiescence and proliferation to promote tissue health. In Drosophila ovaries, transitions follicle stem (FSCs) are exquisitely feeding-dependent. Here, we demonstrate feeding-dependent induction cell differentiation markers, eyes absent (Eya) castor (Cas) in FSCs, a patterning process that does not depend on induction. Instead, FSCs extend micron-scale cytoplasmic projections dictate Eya-Cas patterning. We identify still life sickie as necessary sufficient for FSC projection growth Our results suggest sequential, interdependent events establish long-term patterns precursors, independently

Language: Английский

A single-cell atlas and lineage analysis of the adult Drosophila ovary DOI Creative Commons
Katja Rust, Lauren Byrnes, Kevin Shengyang Yu

et al.

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: Nov. 6, 2020

The Drosophila ovary is a widely used model for germ cell and somatic tissue biology. Here we use single-cell RNA-sequencing (scRNA-seq) to build comprehensive atlas of the adult that contains transcriptional profiles every major type in ovary, including germline stem cells their niche cells, follicle previously undescribed subpopulations escort cells. In addition, identify Gal4 lines with specific expression patterns perform lineage tracing We discover distinct subpopulation able convert response starvation or upon genetic manipulation, knockdown escargot, overactivation mTor Toll signalling.

Language: Английский

Citations

121
Cell polarity opposes Jak/STAT-mediated Escargot activation that drives intratumor heterogeneity in a Drosophila tumor model DOI Creative Commons
Deeptiman Chatterjee, Fei Cong, Xianfeng Wang

et al.

Cell Reports, Journal Year: 2023, Volume and Issue: 42(2), P. 112061 - 112061

Published: Jan. 28, 2023

In proliferating neoplasms, microenvironment-derived selective pressures promote tumor heterogeneity by imparting diverse capacities for growth, differentiation, and invasion. However, what makes a cell respond to signaling cues differently from normal is not well understood. the Drosophila ovarian follicle cells, apicobasal-polarity loss induces heterogeneous epithelial multilayering. When exacerbated oncogenic-Notch expression, this multilayer displays an increased consistency in occurrence of morphologically distinguishable cells adjacent polar cells. Polar release Jak/STAT ligand Unpaired (Upd), response which neighboring polarity-deficient exhibit precursor-like transcriptomic state. Among several regulons active these we could detect further validate expression Snail family transcription factor Escargot (Esg). We also ascertain similar relationship between Upd Esg normally developing ovaries, where establishment polarity determines early follicular differentiation. Overall, our results indicate that epithelial-cell acts as gatekeeper against microenvironmental drive heterogeneity.

Language: Английский

Citations

13
Aquaporins implicated in the cell proliferation and the signaling pathways of cell stemness DOI
Hyun Jun Jung,

Hyo-Ju Jang,

Tae‐Hwan Kwon

et al.

Biochimie, Journal Year: 2021, Volume and Issue: 188, P. 52 - 60

Published: April 21, 2021

Language: Английский

Citations

13
Adult stem cells and niche cells segregate gradually from common precursors that build the adult Drosophila ovary during pupal development DOI Creative Commons
Amy Reilein, Helen V. Kogan, Rachel Misner

et al.

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Sept. 30, 2021

Production of proliferative follicle cells (FCs) and quiescent escort (ECs) by stem (FSCs) in adult Drosophila ovaries is regulated niche signals from anterior (cap cells, ECs) posterior (polar FCs) sources. Here we show that ECs, FSCs, FCs develop common pupal precursors, with different fates acquired progressive separation along the AP axis a graded decline cell proliferation. most derive intermingled (IC) precursors interspersed germline cells. Precursors also accumulate to ICs before engulfing naked cyst projected out germarium form first egg chamber polar FC signaling center. Thus, appropriate numbers spatial organization through expansion together establishment cues guide behavior, rather than rigid early specification events.

Language: Английский

Citations

11
Single-cell expression profile of Drosophila ovarian follicle stem cells illuminates spatial differentiation in the germarium DOI Creative Commons
Zhi Dong, Lan Pang, Zhiguo Liu

et al.

BMC Biology, Journal Year: 2023, Volume and Issue: 21(1)

Published: June 20, 2023

How stem cell populations are organized and regulated within adult tissues is important for understanding cancer origins developing replacement strategies. Paradigms such as mammalian gut cells Drosophila ovarian follicle (FSC) characterized by population asymmetry, in which division differentiation separately processes. These behave stochastically regarding their contributions to derivative also exhibit dynamic spatial heterogeneity. FSCs provide an excellent model how a community of active maintained asymmetry regulated. Here, we use single-cell RNA sequencing profile the gene expression patterns immediate derivatives investigate heterogeneity changes associated with differentiation.

Language: Английский

Citations

4
Underlying Mechanism of Fluoride Inhibits Colonic Gland Cells Proliferation by Inducing an Inflammation Response DOI
Jing Liu, Jing Zhao, Yuling Zhang

et al.

Biological Trace Element Research, Journal Year: 2024, Volume and Issue: unknown

Published: July 12, 2024

Language: Английский

Citations

1
STAT transcription factor is indispensable for oogenesis in silkworm DOI
Shiyuan Wang, Li Zhou, Wenjuan Liang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 278, P. 133864 - 133864

Published: July 15, 2024

Language: Английский

Citations

1
Investigating Adult Stem Cells Through Lineage analyses DOI
Daniel Kalderon

Stem Cell Reviews and Reports, Journal Year: 2021, Volume and Issue: 18(1), P. 2 - 22

Published: Oct. 22, 2021

Language: Английский

Citations

8
Spatial regulation of Drosophila ovarian Follicle Stem Cell division rates and cell cycle transitions DOI Creative Commons
David Melamed,

Aaron Choi,

Amy Reilein

et al.

PLoS Genetics, Journal Year: 2023, Volume and Issue: 19(9), P. e1010965 - e1010965

Published: Sept. 25, 2023

Drosophila ovarian Follicle Stem Cells (FSCs) present a favorable paradigm for understanding how stem cell division and differentiation are balanced in communities where those activities independent. FSCs also allow exploration of this balance is integrated with spatial heterogeneity. Posterior become proliferative (FCs), while anterior quiescent Escort (ECs) at about one fourth the frequency. A single can nevertheless produce both FCs ECs because it move between posterior locations. Studies based on EdU incorporation to approximate rates suggested that divide faster than FSCs. However, direct measures cycle times required ascertain whether FC output requires net flow from posterior. Here, by using live imaging FUCCI cell-cycle reporters, we measured absolute rates. We found more three produced sufficient new cells match production. H2B-RFP dilution studies supported different cycling according A/P location facilitated imaging, showing exchange directions, consistent dynamic equilibrium inferred rate measurements. Inversely graded Wnt JAK-STAT pathway signals regulate FSC FCs. promotes cycling, affording some coordination these activities. When signaling was manipulated be spatially uniform, ratio reduced but remained substantial, only partly explains By markers, prominent G2/M restriction together an G1/S restriction, transitions, PI3 kinase principally stimulates transition.

Language: Английский

Citations

3
Extracellular spreading of Wingless is required for Drosophila oogenesis DOI Creative Commons
Xiaoxi Wang, Kimberly S. LaFever, Indrayani Waghmare

et al.

PLoS Genetics, Journal Year: 2021, Volume and Issue: 17(4), P. e1009469 - e1009469

Published: April 2, 2021

Recent studies have investigated whether the Wnt family of extracellular ligands can signal at long range, spreading from their source and acting as morphogens, or they only in a juxtacrine manner to neighboring cells. The original evidence for long-range signaling arose Wg, Drosophila protein, which patterns wing disc over several cell diameters central Wg ligand. However, requirement patterning was called into question when it reported that replacing secreted protein with membrane-tethered version, NRT-Wg, results flies normally patterned wings. We others previously spreads ovary about 50 μm 5 diameters, cap cells follicle stem (FSCs) stimulates FSC proliferation. used NRT-wg analyze consequence tethering homozygous are sickly, but we found hemizygous NRT-wg/null flies, carrying one copy tethered Wingless, were significantly healthier. Despite overall improved health, these displayed dramatic reductions fertility Further, proliferation nearly undetectable wg locus converted adults, resulting germarium phenotype consistent loss-of-function phenotype. conclude its promote

Language: Английский

Citations

6