Fly Cell Atlas: A single-nucleus transcriptomic atlas of the adult fruit fly

Science, Journal Year: 2022, Volume and Issue: 375(6584)

Published: March 3, 2022

For more than 100 years, the fruit fly

Language: Английский

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Non-canonical odor coding in the mosquito

Cell, Journal Year: 2022, Volume and Issue: 185(17), P. 3104 - 3123.e28

Published: Aug. 1, 2022

Aedes aegypti mosquitoes are a persistent human foe, transmitting arboviruses including dengue when they feed on blood. Mosquitoes intensely attracted to body odor and carbon dioxide, which detect using ionotropic chemosensory receptors encoded by three large multi-gene families. Genetic mutations that disrupt the olfactory system have modest effects attraction, suggesting redundancy in coding. The canonical view is sensory neurons each express single receptor defines its ligand selectivity. We discovered Ae. uses different organizational principle, with many co-expressing multiple genes. In vivo electrophysiology demonstrates broad ligand-sensitivity of mosquito depends this non-canonical co-expression. afforded an co-express may increase robustness explain our long-standing inability detection humans mosquitoes.

Language: Английский

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Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons

eLife, Journal Year: 2022, Volume and Issue: 11

Published: April 20, 2022

olfactory neurons have long been thought to express only one chemosensory receptor gene family. There are two main families in

Language: Английский

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Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq of Drosophila testes

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Feb. 16, 2023

Proper differentiation of sperm from germline stem cells, essential for production the next generation, requires dramatic changes in gene expression that drive remodeling almost all cellular components, chromatin to organelles cell shape itself. Here, we provide a single nucleus and RNA-seq resource covering spermatogenesis Drosophila starting in-depth analysis adult testis (snRNA-seq) data Fly Cell Atlas (FCA) study. With over 44,000 nuclei 6000 cells analyzed, identification rare types, mapping intermediate steps differentiation, potential identify new factors impacting fertility or controlling supporting somatic cells. We justify assignment key types using combinations known markers, situ hybridization, extant protein traps. Comparison datasets proved particularly revealing dynamic developmental transitions differentiation. To complement web-based portals hosted by FCA, compatible with commonly used software such as Seurat Monocle. The foundation provided here will enable communities studying interrogate candidate genes test function vivo.

Language: Английский

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Transcriptional complexity in the insect central complex: single nuclei RNA-sequencing of adult brain neurons derived from type 2 neuroblasts

Published: Feb. 27, 2025

In both invertebrates such as Drosophila and vertebrates mouse or human, the brain contains most diverse population of cell types any tissue. It is generally accepted that transcriptional diversity an early step in generating neuronal glial diversity, followed by establishment a unique gene expression profile determines morphology, connectivity, function. , there are two neural stem cells, called Type 1 (T1) 2 (T2) neuroblasts. contrast to T1 neuroblasts, T2 neuroblasts generate intermediate progenitors (INPs) expand number types. The T2-derived neurons contributes large portion central complex (CX), conserved region plays role sensorimotor integration. Recent work has revealed much connectome CX, but how this assembled remains unclear. Mapping derived from necessary linking assembly adult brain. Here we perform single nuclei RNA sequencing neuroblast-derived glia. We identify clusters containing all known classes glia, male/female enriched, 161 neuron-specific clusters. map neurotransmitter neuropeptide transcription factor combinatorial codes for each cluster (presumptive neuron subtype). This directs functional studies determine whether code specifies distinct type within CX. several columnar subtypes (NPF+ AstA+) closely related Our data support hypothesis represents one few subtypes.

Language: Английский

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Single-cell transcriptomes of developing and adult olfactory receptor neurons in Drosophila

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Feb. 8, 2021

Recognition of environmental cues is essential for the survival all organisms. Transcriptional changes occur to enable generation and function neural circuits underlying sensory perception. To gain insight into these changes, we generated single-cell transcriptomes Drosophila olfactory- (ORNs), thermo-, hygro-sensory neurons at an early developmental adult stage using single-nucleus RNA sequencing. We discovered that ORNs maintain expression same olfactory receptors across development. Using receptor computational approaches, matched transcriptomic clusters corresponding anatomically physiologically defined neuron types multiple stages. found cell-type-specific partly reflected axon trajectory choices in development modality adults. uncovered stage-specific genes could regulate wiring responses distinct ORN types. Collectively, our data reveal features biology provide a resource future studies their physiology.

Language: Английский

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The continuum of Drosophila embryonic development at single-cell resolution

Science, Journal Year: 2022, Volume and Issue: 377(6606)

Published: Aug. 4, 2022

Drosophila melanogaster is a powerful, long-standing model for metazoan development and gene regulation. We profiled chromatin accessibility in almost 1 million expression half nuclei from overlapping windows spanning the entirety of embryogenesis. Leveraging developmental asynchronicity within embryo collections, we applied deep neural networks to infer age each nucleus, resulting continuous, multimodal views molecular cellular transitions absolute time. identify cell lineages; their relationships; link dynamic changes enhancer usage, transcription factor (TF) expression, TFs’ cognate motifs. With these data, dynamics usage can be explored across lineages at scale minutes, including precise like zygotic genome activation.

Language: Английский

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Context-dependent reversal of odorant preference is driven by inversion of the response in a single sensory neuron type

PLoS Biology, Journal Year: 2022, Volume and Issue: 20(6), P. e3001677 - e3001677

Published: June 13, 2022

The valence and salience of individual odorants are modulated by an animal’s innate preferences, learned associations, internal state, as well the context odorant presentation. mechanisms underlying context-dependent flexibility in odor not fully understood. Here, we show that behavioral response Caenorhabditis elegans to bacterially produced medium-chain alcohols switches from attraction avoidance when presented background a subset additional attractive chemicals. This reversal preference is driven cell-autonomous inversion these single AWC olfactory neuron pair. We find while inhibit neurons drive attraction, instead activate promote second AWC-sensed odorant. opposing responses via engagement distinct odorant-directed signal transduction pathways within AWC. Our results indicate recruitment alternative intracellular signaling sensory type conveys opposite hedonic valences, thereby providing robust mechanism for encoding discrimination at periphery.

Language: Английский

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Fly Cell Atlas: a single-cell transcriptomic atlas of the adult fruit fly

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: July 5, 2021

Abstract The ability to obtain single cell transcriptomes for stable types and dynamic states is ushering in a new era biology. We created the Tabula Drosophilae , atlas of adult fruit fly which includes 580k cells from 15 individually dissected sexed tissues as well entire head body. Over 100 researchers community contributed annotations >250 distinct across all tissues. provide an in-depth analysis type-related gene signatures transcription factor markers, sexual dimorphism, whole animal. Analysis common that are shared between tissues, such blood muscle cells, allowed discovery rare tissue-specific subtypes. This provides valuable resource Drosophila serves comprehensive reference study genetic perturbations disease models at resolution.

Language: Английский

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Spatial transcriptomic reconstruction of the mouse olfactory glomerular map suggests principles of odor processing

Nature Neuroscience, Journal Year: 2022, Volume and Issue: 25(4), P. 484 - 492

Published: March 21, 2022

Language: Английский

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