Clonally related, Notch-differentiated spinal neurons integrate into distinct circuits DOI Creative Commons
Saul Bello‐Rojas, Martha W. Bagnall

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Dec. 29, 2022

Shared lineage has diverse effects on patterns of neuronal connectivity. In mammalian cortex, excitatory sister neurons assemble into shared microcircuits. Drosophila, in contrast, with different levels Notch expression (NotchON/NotchOFF) develop distinct identities and diverge separate circuits. Notch-differentiated have been observed vertebrate spinal cord cerebellum, but whether they integrate or circuits remains unknown. Here, we evaluate how V2a (NotchOFF)/V2b (NotchON) the zebrafish Using an vivo labeling approach, identified pairs V2a/b born from individual Vsx1+ progenitors that somata close proximity to each other similar axonal trajectories. However, paired whole-cell electrophysiology optogenetics revealed receive input presynaptic sources, do not communicate other, connect largely targets. These results resemble divergent connectivity Drosophila represent first evidence circuit integration a system.

Language: Английский

A complete temporal transcription factor series in the fly visual system DOI
Νικόλαος Κωνσταντινίδης, Isabel Holguera, Anthony Rossi

et al.

Nature, Journal Year: 2022, Volume and Issue: 604(7905), P. 316 - 322

Published: April 6, 2022

Language: Английский

Citations

99
Connectomic reconstruction of a female Drosophila ventral nerve cord DOI
Anthony W. Azevedo, Ellen Lesser, Jasper S. Phelps

et al.

Nature, Journal Year: 2024, Volume and Issue: 631(8020), P. 360 - 368

Published: June 26, 2024

Language: Английский

Citations

37
Synaptic architecture of leg and wing premotor control networks in Drosophila DOI
Ellen Lesser, Anthony W. Azevedo, Jasper S. Phelps

et al.

Nature, Journal Year: 2024, Volume and Issue: 631(8020), P. 369 - 377

Published: June 26, 2024

Language: Английский

Citations

25
Connectomics and the neural basis of behaviour DOI Creative Commons
Dana S. Galili, Gregory S.X.E. Jefferis, Marta Costa

et al.

Current Opinion in Insect Science, Journal Year: 2022, Volume and Issue: 54, P. 100968 - 100968

Published: Sept. 13, 2022

Methods to acquire and process synaptic-resolution electron-microscopy datasets have progressed very rapidly, allowing production annotation of larger, more complete connectomes. More accurate neuronal matching techniques are enriching cell type data with gene expression, neuron activity, behaviour developmental information, providing ways test hypotheses circuit function. In a variety behaviours such as learned innate olfaction, navigation sexual behaviour, connectomics has already revealed interconnected modules hierarchical structure, recurrence integration sensory streams. Comparing individual connectomes determine which features robust variable is one key research area; new work in comparative across development, experience, sex species will establish strong links between connectivity brain

Language: Английский

Citations

43
Organization of the gravity-sensing system in zebrafish DOI Creative Commons
Zhikai Liu, David G. C. Hildebrand, Joshua Morgan

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Aug. 27, 2022

Motor circuits develop in sequence from those governing fast movements to slow. Here we examine whether upstream sensory are organized by similar principles. Using serial-section electron microscopy larval zebrafish, generated a complete map of the gravity-sensing (utricular) system spanning inner ear brainstem. We find that both tuning and developmental organizing principles vestibular topography. Patterned rostrocaudal innervation hair cells afferents creates an anatomically inferred directional utricular ganglion, forming segregated pathways for rostral caudal tilt. Furthermore, mediolateral axis ganglion is linked neuronal temporal dynamics. Early-born carrying phasic information preferentially excite escape circuits, whereas later-born tonic signals slower postural oculomotor circuits. These results demonstrate direction dynamics, aligning them with downstream motor behaviors.

Language: Английский

Citations

40
Metamorphosis of memory circuits in Drosophila reveals a strategy for evolving a larval brain DOI Creative Commons
James W. Truman,

Jacquelyn Price,

Rosa Linda Miyares

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Jan. 25, 2023

Mushroom bodies (MB) of adult Drosophila have a core thousands Kenyon neurons; axons the early-born g class form medial lobe and those from later-born α'β' αβ classes both vertical lobes. The larva, however, hatches with only γ neurons forms 'facsimile' using larval-specific axon branches its neurons. MB input (MBINs) output (MBONs) divide neuron lobes into discrete computational compartments. larva has 10 such compartments while 16. We determined fates 28 32 MBONs MBINs that define larval Seven are subsequently incorporated MB; four their die, 12 MBINs/MBONs remodel to function in remaining three specific. At metamorphosis MBIN/MBONs trans-differentiate, leaving for other brain circuits. made de novo MBONs/MBINs recruited pools adult-specific combination cell death, compartment shifting, trans-differentiation, recruitment new result no MBIN-MBON connections being maintained through metamorphosis. this simple level, then, we find anatomical substrate memory trace persisting adult. phenotype trans-differentiating represents evolutionarily ancestral is derived adaptation stage. These cells arise primarily within lineages also produce permanent MBONs, suggesting specifying factors may allow information related birth-order or sibling identity be interpreted modified manner these acquire phenotypic modifications. loss at then allows revert functions

Language: Английский

Citations

37
Temporal and Notch identity determine layer targeting and synapse location of medulla neurons DOI Creative Commons
Isabel Holguera, Yee‐Chun Chen, Yu‐Chieh Chen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

How specification mechanisms that generate neural diversity translate into specific neuronal targeting, connectivity, and function in the adult brain is not understood. In medulla region of Drosophila optic lobe, progenitors different neurons a fixed order by sequentially expressing series temporal transcription factors as they age. Then, Notch signaling intermediate further diversifies progeny. By establishing birth neurons, we found their identity correlates with depth neuropil targeting brain, for both local interneurons projection neurons. We show this identity-dependent unfolds early development genetically determined. leveraging Electron Microscopy reconstruction fly determined synapse location lobe neuropils find it significantly associated status. Moreover, all putative same predicted share similar location, indicating ensembles layers encode visual functions. conclusion, status can predict function, linking developmental patterning connectivity functional features brain.

Language: Английский

Citations

1
Lineages to circuits: the developmental and evolutionary architecture of information channels into the central complex DOI Creative Commons

Pratyush Kandimalla,

Jaison J. Omoto, Elizabeth J. Hong

et al.

Journal of Comparative Physiology A, Journal Year: 2023, Volume and Issue: 209(4), P. 679 - 720

Published: March 17, 2023

The representation and integration of internal external cues is crucial for any organism to execute appropriate behaviors. In insects, a highly conserved region the brain, central complex (CX), functions in spatial information behavioral states, as well transformation this into desired navigational commands. How does relatively invariant structure enable incorporation from diversity anatomical, behavioral, ecological niches occupied by insects? Here, we examine input channels CX context their development evolution. Insect brains develop ~ 100 neuroblasts per hemisphere that divide systematically form "lineages" sister neurons, project target neuropils along anatomically characteristic tracts. Overlaying developmental tract onto recently generated Drosophila "hemibrain" connectome integrating with anatomical physiological recording neurons other species, observe neuropil lineage-specific innervation, connectivity, activity profiles channels. We posit proliferative potential lineage-based architecture modification neural networks across existing, novel, deprecated modalities species-specific manner, thus forming substrate evolution diversification insect circuits.

Language: Английский

Citations

21
Neuronal birthdate reveals topography in a vestibular brainstem circuit for gaze stabilization DOI Creative Commons
Dena Goldblatt, Stephanie Huang, Marie R. Greaney

et al.

Current Biology, Journal Year: 2023, Volume and Issue: 33(7), P. 1265 - 1281.e7

Published: March 15, 2023

Across the nervous system, neurons with similar attributes are topographically organized. This topography reflects developmental pressures. Oddly, vestibular (balance) nuclei thought to be disorganized. By measuring activity in birthdated neurons, we revealed a functional map within central projection nucleus that stabilizes gaze larval zebrafish. We first discovered both somatic position and stimulus selectivity follow neuron birthdate. Next, electron microscopy loss-of-function assays, found patterns of peripheral innervation were similarly organized by Finally, birthdate spatial axonal arborization synapse formation outputs. Collectively, find development reveals previously hidden organization input, processing, output layers highly conserved vertebrate sensorimotor circuit. The temporal uncover constrain mechanisms may specify fate, function, vestibulo-ocular reflex neurons. More broadly, our data suggest that, like invertebrates, assemble architecture.

Language: Английский

Citations

17
Synaptic architecture of leg and wing premotor control networks inDrosophila DOI Creative Commons
Ellen Lesser, Anthony W. Azevedo, Jasper S. Phelps

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 31, 2023

Animal movement is controlled by motor neurons (MNs), which project out of the central nervous system to activate muscles. MN activity coordinated complex premotor networks that allow individual muscles contribute many different behaviors. Here, we use connectomics analyze wiring logic circuits controlling

Language: Английский

Citations

17