A core of functional complementary bacteria infects oysters in Pacific Oyster Mortality Syndrome DOI Creative Commons
Camille Clérissi, Xing Luo,

Aude Lucasson

et al.

Animal Microbiome, Journal Year: 2023, Volume and Issue: 5(1)

Published: May 3, 2023

Abstract Background The Pacific oyster Crassostrea gigas is one of the main cultivated invertebrate species worldwide. Since 2008, juveniles have been confronted with a lethal syndrome known as Oyster Mortality Syndrome (POMS). POMS polymicrobial disease initiated by primary infection herpesvirus OsHV-1 µVar that creates an immunocompromised state and evolves towards secondary fatal bacteremia. Results In present article, we describe implementation unprecedented combination metabarcoding metatranscriptomic approaches to show sequence events in pathogenesis conserved across infectious environments. We also identified core bacterial consortium which, together µVar, forms pathobiota. This characterized high transcriptional activities complementary metabolic functions exploit host’s resources. A significant specificity was highlighted at genus level, suggesting low competition for nutrients between members bacteria. Conclusions Lack bacteria might favor colonization host tissues contribute conservation pathobiota distinct

Language: Английский

Conversion of Phytochemicals by Lactobacilli: (Phospho)-β-glucosidases Are Specific for Glucosylated Phytochemicals Rather than Disaccharides DOI
Monika Dymarska,

Anna Widenmann,

Kristin E. Low

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(10), P. 5428 - 5438

Published: Feb. 28, 2024

Food-fermenting lactobacilli convert glycosylated phytochemicals to glycosyl hydrolases and thereby alter their biological activity. This study aimed investigate the microbial transformation of β-glucosides in comparison with utilization cellobiose. Four homofermentative four heterofermentative were selected represent metabolic diversity Lactobacillaceae. The genomes Lactobacillus crispatus, Companilactobacillus paralimentarius, Lacticaseibacillus paracasei, Lactiplantibacillus plantarum encoded for 8 22 enzymes, predominantly phospho-β-glucosidases, predicted activity on β-glucosides. Levilactobacillus hammesii Furfurilactobacillus milii 3 β-glucosidases, rossiae one, Fructilactobacillus sanfranciscensis none. hydrolysis amygdalin, esculin, salicin, glucosides quercetin genistein, ginsenosides demonstrated that several strains hydrolyzed but not Taken together, carbohydrate-active enzymes food-fermenting are specific glycosides phytochemicals.

Language: Английский

Citations

7
VIRGO2: Unveiling the Functional and Ecological Complexity of the Vaginal Microbiome with an Enhanced Non-Redundant Gene Catalog DOI Creative Commons
Michael France, Issac Chaudry,

Lindsay Rutt

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Abstract Despite the importance of cervicovaginal microbiome, mechanisms that govern its composition and drive impact on host physiology remain poorly understood. This study expands our understanding function ecology vaginal microbiome using VIRGO2, an enhanced non-redundant gene catalog comprising over 1.7 million well-annotated genes from body-site specific microbes viruses. Analyses VIRGO2 revealed novel insights, including identification previously uncharacterized bacteria, features mycobiome phageome, differential expression bacterial carbohydrate catabolic genes. Constructed 2,500 metagenomes 4,000 genomes, broadens geographic representation microbial diversity compared to predecessor. updated enables more precise profiling taxonomic functional metagenomic metatranscriptomic datasets. is a critical resource for integrative analyses communities their interactions with tissues, thereby enhancing mechanistic health disease.

Language: Английский

Citations

1
Bumblebees with the socially transmitted microbiome: A novel model organism for gut microbiota research DOI
Zijing Zhang, Hao Zheng

Insect Science, Journal Year: 2022, Volume and Issue: 29(4), P. 958 - 976

Published: May 14, 2022

Abstract Eusocial bumble and honey bees are important pollinators for global ecology the agricultural economy. Although both possess similar host‐restricted gut microbiota, they differ in aspects of morphology, autonomy, physiology, behavior, life cycle. The social bee bacteria exhibit host specificity that is likely a result long‐term co‐evolution. unique cycle bumblebees key acquisition development their affects strain‐level diversity core bacterial species. Studies on bumblebee show retain less functional capacity carbohydrate metabolism compared with honeybee. We discuss potential roles microbiota against pathogenic threats application host‐specific probiotics bumblebees. Given advantages microbiome, including simple structure specificity, ease manipulating colonies, we propose may provide valuable system understanding general principles host–microbe interactions, gut–brain axis, vertical transmission.

Language: Английский

Citations

25
From microbiome composition to functional engineering, one step at a time DOI
Sebastian D. Burz, Senka Čaušević, Alma Dal Co

et al.

Microbiology and Molecular Biology Reviews, Journal Year: 2023, Volume and Issue: 87(4)

Published: Nov. 10, 2023

SUMMARY Communities of microorganisms (microbiota) are present in all habitats on Earth and relevant for agriculture, health, climate. Deciphering the mechanisms that determine microbiota dynamics functioning within context their respective environments or hosts (the microbiomes) is crucially important. However, sheer taxonomic, metabolic, functional, spatial complexity most microbiomes poses substantial challenges to advancing our knowledge these mechanisms. While nucleic acid sequencing technologies can chart composition with high precision, we mostly lack information about functional roles interactions each strain a given microbiome. This limits ability predict microbiome function natural and, case dysfunction dysbiosis, redirect onto stable paths. Here, will discuss systematic approach (dubbed N + 1/N−1 concept) enable step-by-step dissection assembly functioning, as well intervention procedures introduce eliminate one particular microbial at time. The N+1/N−1 concept informed by invasion events selects culturable, genetically accessible microbes well-annotated genomes proliferation decline defined synthetic and/or complex microbiota. enables harnessing classical microbiological diversity approaches, omics tools mathematical modeling decipher underlying outcomes. Application this further provides stepping stones benchmarks structure analyses more strategies.

Language: Английский

Citations

16
A core of functional complementary bacteria infects oysters in Pacific Oyster Mortality Syndrome DOI Creative Commons
Camille Clérissi, Xing Luo,

Aude Lucasson

et al.

Animal Microbiome, Journal Year: 2023, Volume and Issue: 5(1)

Published: May 3, 2023

Abstract Background The Pacific oyster Crassostrea gigas is one of the main cultivated invertebrate species worldwide. Since 2008, juveniles have been confronted with a lethal syndrome known as Oyster Mortality Syndrome (POMS). POMS polymicrobial disease initiated by primary infection herpesvirus OsHV-1 µVar that creates an immunocompromised state and evolves towards secondary fatal bacteremia. Results In present article, we describe implementation unprecedented combination metabarcoding metatranscriptomic approaches to show sequence events in pathogenesis conserved across infectious environments. We also identified core bacterial consortium which, together µVar, forms pathobiota. This characterized high transcriptional activities complementary metabolic functions exploit host’s resources. A significant specificity was highlighted at genus level, suggesting low competition for nutrients between members bacteria. Conclusions Lack bacteria might favor colonization host tissues contribute conservation pathobiota distinct

Language: Английский

Citations

14