Cell Calcium, Journal Year: 2025, Volume and Issue: unknown, P. 103014 - 103014
Published: March 1, 2025
Language: Английский
Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)
Published: Aug. 1, 2023
Abstract The present work examined the effect of oral administration rutin and its combination with metformin, an antidiabetic drug on blood glucose, total cholesterol triglycerides level vascular function in streptozotocin (STZ) -induced diabetic rats. Male Sprague Dawley rats were rendered by a single intraperitoneal injection STZ (50 mg/kg). Rutin metformin orally administered to at dose 100 mg/kg 300 body weight/day, respectively, for 4 weeks. Plasma analysis was conducted determine changes plasma glucose lipid levels. Rat aortic ring reactivity response endothelium-dependent (acetylcholine, ACh) endothelium-independent (sodium nitroprusside, SNP) relaxants, α1-adrenergic agonist phenylephrine (PE) recorded. Histology pancreas, liver kidney evaluated. In results, alone has led significant improvements triglyceride levels compared group. Diabetic rings showed significantly greater contraction PE, less relaxation ACh SNP. Treatment reduced PE-induced increased ACh-induced SNP-induced diabetes when or alone. Significant histological seen therapy. conclusion, therapy most potentiality restoring as well function.
Language: Английский
Citations
9
Trends in Pharmacological Sciences, Journal Year: 2024, Volume and Issue: 45(9), P. 798 - 810
Published: Aug. 9, 2024
Language: Английский
Citations
3
Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 224, P. 39 - 49
Published: Aug. 10, 2024
The pathogenesis of intervertebral disc degeneration (IVDD) involves complex signaling networks and various effector molecules, our understanding the IVDD is limited. Hypoxia inducible factor-1α (HIF-1α) closely related to IVDD, there excessive oxidative stress concurrent with IVDD. In this study, we found that HIF-1α could protect nucleus pulposus cells from by reversing imbalance between oxidants antioxidants thus mitigating stress-induced mitochondrial impairment. With further exploration, pyruvate dehydrogenase kinase 1 (PDK-1) was involved in protective effect on under stress. We suggested preserve integrity activate glycolysis via PDK-1, addition DCA, a PDK-1 inhibitor, blunt HIF-1α. addition, HIF-1α/PDK-1 regulatory axis also confirmed vivo through knockout mice model. Therefore, propose protects maintaining enriching insight into mechanism against providing novel therapeutic target for treatment
Language: Английский
Citations
3
Published: Jan. 7, 2025
<p dir="ltr">Psoriatic arthritis mutilans (PAM) and atopic dermatitis (AD) are two skin disorders. PAM is the most severe rarest form of psoriatic arthritis, it characterized by osteolysis joint erosion, leading to permanent disability. There no clear susceptibility genes identified for PAM. AD, a chronic inflammatory condition, influenced genetic environmental factors, resulting in barrier dysfunction, inflammation, immune dysregulation. In while risk factors have been explained, these explain only modest proportion heritability. The pathogenesis varies different populations, such as those African descent.</p><p dir="ltr">In this thesis, I explore molecular underpinnings both AD through next-generation sequencing functional studies. We aim uncover novel involved disease development. research goals were achieved following four studies included thesis.</p><p study I, we performed Next-generation Nordic cohort (n = 61) basis uncovered rare variants NADPH oxidase 4 (NOX4) gene suggested NOX4 candidate gene. an enzyme responsible generating reactive oxygen species (ROS) production osteoclast differentiation. Functional showed that upregulate ROS levels patient- derived osteoclasts, zebrafish models HEK293 cells. All data collected linked dysfunction proposed gene, offering potential therapeutic target modulating addressing PAM.</p><p II, shifted focus exploring its origins Ethiopian family with multiple affected members. Notably, mutations FLG Ethiopians, highlighting need investigate alternative contributors population. Whole-genome revealed co-segregating family, FLG2 - p.D13Y NOD2 p.A918S genes. Further genotyping case-control significant association variant. Additionally, previously p.A849V p.G908R also patients. These findings highlight importance etiology particularly populations.</p><p III, further explored role 2-amino ethanethiol dioxygenase (ADO) AD. idea arises from previous which GWAS variant was promoter ADO published Chromosome conformation capture (Capture Hi-C). expression significantly higher lesional compared non-lesional Zebrafish vitro demonstrated dysregulation impairs function enhances inflammation. This suggests plays critical maintaining homeostasis, contributing pathogenesis.</p><p IV, investigated gene's Swedish population, focusing on p.S2377X Genotypic analysis independent cohorts between reduced observed tissue carriers. underscores contribution risk, within population.</p><p dir="ltr">Collectively, thesis broadens understanding Novel insights provided identifying factor PAM, presenting first lead pinpointing condition. Furthermore, highlighted roles FLG2, NOD2, cause.</p><p dir="ltr">Genome serves tool identify cause diseases; Subsequent new into treatment approaches managing diseases.</p><h3>List scientific papers</h3><p dir="ltr">I. Rare coding link high mutilans<br><b>Sailan Wang</b>, Pernilla Nikamo, Leena Laasonen, Bjorn Gudbjornsson, Leif Ejstrup, Lars Iversen, Ulla Lindqvist, Jessica J Alm, Jesper Eisfeldt, Xiaowei Zheng, Sergiu-Bogdan Catrina, Fulya Taylan, Raquel Vaz, Mona Ståhle Isabel Tapia-Paez EMBO Molecular Medicine.2024;16(3):596-615. <a href="https://doi.org/10.1038/s44321-024-00035-z">https://doi.org/10.1038/s44321-024-00035-z</a><br><br>II. Uncommon associated population</p><p dir="ltr"><b>Sailan Julia K. Elmgren, Samina Asad, Marlene Ek, Kassahun Bilcha, Annisa Befekadu, Carl-Fredrik Wahlgren, Magnus Nordenskjöld, Tapia-Paez* Maria Bradley *. JID Innovations. 2024 Apr 29;4(4):100284. href="https://doi.org/10.1016/j.xjidi.2024.100284">https://doi.org/10.1016/j.xjidi.2024.100284</a><br><br>III. cysteamine dermatitis</p><p Josefin Lysell, Pelin Sahlén, Nordenskjold, Bradley* [Manuscript]<br><br>IV. patients</p><p Mahsa Tayefi, Axel Svedbom, Carl- Fredrik Emma Johansson, [Manuscript]</p><p dir="ltr">* Authors contributed equally</p>
Language: Английский
Citations
0
Cell Calcium, Journal Year: 2025, Volume and Issue: unknown, P. 103014 - 103014
Published: March 1, 2025
Language: Английский
Citations
0