A neurotransmitter atlas ofC. elegansmales and hermaphrodites DOI Creative Commons
Chen Wang,

Berta Vidal,

Surojit Sural

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 25, 2023

ABSTRACT Mapping neurotransmitter identities to neurons is key understanding information flow in a nervous system. It also provides valuable entry points for studying the development and plasticity of neuronal identity features. In C. elegans system, have been largely assigned by expression pattern analysis pathway genes that encode biosynthetic enzymes or transporters. However, many these assignments relied on multicopy reporter transgenes may lack relevant cis -regulatory therefore not provide an accurate picture usage. We analyzed patterns 16 CRISPR/Cas9-engineered knock-in strains all main types neurotransmitters (glutamate, acetylcholine, GABA, serotonin, dopamine, tyramine, octopamine) both hermaphrodite male. Our reveals novel sites systems within glia, as well non-neural cells. The resulting atlas defines be exclusively neuropeptidergic, substantially expands repertoire capable co-transmitting multiple neurotransmitters, identifies uptake monoaminergic neurotransmitters. Furthermore, we observed unusual co-expression synthesis genes, suggesting existence transmitters. results what constitutes most extensive whole-animal-wide map usage date, paving way better communication specification .

Language: Английский

Nervous system-wide analysis of all C. elegans cadherins reveals neuron-specific functions across multiple anatomical scales DOI Creative Commons
Maryam Majeed, Chien‐Po Liao, Oliver Hobert

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 8, 2024

Differential expression of cell adhesion proteins is a hallmark type diversity across the animal kingdom. Gene family-wide characterization their organismal and function are, however, lacking. We established an atlas entire set 12 cadherin gene family members in nematode C. elegans, testing hypothesis that this define combinatorial codes may drive nervous system assembly. This reveals differential genomically tagged genes neuronal classes, dichotomy between broadly- narrowly-expressed cadherins, several context-dependent temporal transitions development. Engineered mutant null alleles cadherins were analyzed for defects morphology, behavior, soma positions, neurite neighborhood topology fasciculation, localization synapses many parts system. analysis revealed restricted pattern differentiation at discrete subsets anatomical scales, including novel role experience-dependent electrical synapse formation. In total, our results perspectives on deployment function.

Language: Английский

Citations

1
A neurotransmitter atlas of C. elegans males and hermaphrodites DOI Open Access
Chen Wang,

Berta Vidal,

Surojit Sural

et al.

Published: Sept. 12, 2024

Mapping neurotransmitter identities to neurons is key understanding information flow in a nervous system. It also provides valuable entry points for studying the development and plasticity of neuronal identity features. In C. elegans system, have been largely assigned by expression pattern analysis pathway genes that encode biosynthetic enzymes or transporters. However, many these assignments relied on multicopy reporter transgenes may lack relevant cis -regulatory therefore not provide an accurate picture usage. We analyzed patterns 16 CRISPR/Cas9-engineered knock-in strains all main types neurotransmitters (glutamate, acetylcholine, GABA, serotonin, dopamine, tyramine, octopamine) both hermaphrodite male. Our reveals novel sites systems within glia, as well non-neural cells. The resulting atlas defines be exclusively neuropeptidergic, substantially expands repertoire capable co-transmitting multiple neurotransmitters, identifies uptake monoaminergic neurotransmitters. Furthermore, we observed unusual co-expression synthesis genes, suggesting existence transmitters. results what constitutes most extensive whole-animal-wide map usage date, paving way better communication specification .

Language: Английский

Citations

1
Ageing, Proteostasis, and the Gut: Insights into Neurological Health and Disease DOI

Mahmood Akbar,

Pranoy Toppo,

Aamir Nazir

et al.

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: unknown, P. 102504 - 102504

Published: Sept. 1, 2024

Language: Английский

Citations

1
Almost 40 years of studying homeobox genes in C. elegans DOI
Paschalis Kratsios, Oliver Hobert

Development, Journal Year: 2024, Volume and Issue: 151(21)

Published: Oct. 30, 2024

ABSTRACT Homeobox genes are among the most deeply conserved families of transcription factor-encoding genes. Following their discovery in Drosophila, homeobox arrived on Caenorhabditis elegans stage with a vengeance. Between 1988 and 1990, just few years after initial flies vertebrates, positional cloning sequence-based searches showed that C. contains HOX cluster genes, an apparent surprise given simplicity non-segmented body plan nematode, as well many other non-clustered all major subfamilies (e.g. LIM, POU, etc.). Not quite 40 later, we have exceptionally deep understanding homeodomain protein expression function elegans, revealing prevalent role nervous system development. In this Spotlight, provide historical perspective non-comprehensive journey through field discuss open questions future directions.

Language: Английский

Citations

1
A neurotransmitter atlas ofC. elegansmales and hermaphrodites DOI Creative Commons
Chen Wang,

Berta Vidal,

Surojit Sural

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 25, 2023

ABSTRACT Mapping neurotransmitter identities to neurons is key understanding information flow in a nervous system. It also provides valuable entry points for studying the development and plasticity of neuronal identity features. In C. elegans system, have been largely assigned by expression pattern analysis pathway genes that encode biosynthetic enzymes or transporters. However, many these assignments relied on multicopy reporter transgenes may lack relevant cis -regulatory therefore not provide an accurate picture usage. We analyzed patterns 16 CRISPR/Cas9-engineered knock-in strains all main types neurotransmitters (glutamate, acetylcholine, GABA, serotonin, dopamine, tyramine, octopamine) both hermaphrodite male. Our reveals novel sites systems within glia, as well non-neural cells. The resulting atlas defines be exclusively neuropeptidergic, substantially expands repertoire capable co-transmitting multiple neurotransmitters, identifies uptake monoaminergic neurotransmitters. Furthermore, we observed unusual co-expression synthesis genes, suggesting existence transmitters. results what constitutes most extensive whole-animal-wide map usage date, paving way better communication specification .

Language: Английский

Citations

3