Science China Life Sciences, Journal Year: 2024, Volume and Issue: 67(11), P. 2426 - 2443
Published: July 23, 2024
Language: Английский
Genetics, Journal Year: 2025, Volume and Issue: unknown
Published: May 6, 2025
Abstract Heterozygous inversion breakpoints inhibit the formation of chiasmata in their vicinity, and it has been suggested that they do so through same mechanism also causes interference between chiasmata. In this paper, I therefore extend my earlier model chiasma to account for pericentric heterokaryotypes. Using analyze recombination sterility datasets Drosophila melanogaster, find support hypothesis interfere with way degree other do. breakpoints, like chiasmata, appear show negative pericentromeric region, positive elsewhere. discuss implications these findings light recent HEI10 coarsening hypothesis, conclude some remarks about evolutionary origin interference.
Language: Английский
Citations
0
Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(19)
Published: May 7, 2025
During meiosis, the correct pairing, synapsis, and recombination of homologous chromosome pairs is critical for fertility sexual eukaryotes. These processes are challenged in polyploids, which possess additional copies each chromosome. Polyploidy thus provides a unique context to study how evolution can modify meiotic programs response challenges. We previously observed that newly formed (neo-)polyploids Arabidopsis arenosa , synapsis defects precede chromosomes associating aberrant multivalent univalent configurations. Here, we dynamics genotypes with varying levels stability ask whether efficient synaptic progression key component evolving stable tetraploid meiosis. develop method quantify using foci pro-crossover factor HEI10 as reference. initially appears at many small before accumulating only crossover sites. In diploids, this transition begins while significant asynapsis still present, though it quickly declines accumulates fewer foci. neo-tetraploids, suboptimal elongation initiation sites stalled perhaps due defective occurs onset accumulation. established tetraploids, accumulation when near complete, suggesting enhanced HEI10/synapsis codynamics (even compared diploids). Hybrids generated by crossing neo- tetraploids exhibit intermediate phenotypes. find extent correlates positively numbers, frequency multivalents univalents, disturb segregation. Our work supports hypothesis improving efficiency important polyploid stability.
Language: Английский
Citations
0
Nature Plants, Journal Year: 2024, Volume and Issue: 10(3), P. 453 - 468
Published: Feb. 20, 2024
Language: Английский
Citations
3
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 8, 2024
Abstract During meiosis, the parental chromosomes are drawn together to enable exchange of genetic information. Chromosomes aligned through assembly a conserved interface, synaptonemal complex, composed central region that forms between two parallel chromosomal backbones called axes. Here we identify axis-central interface in C. elegans , containing positive patch on axis component HIM-3 and C-terminus protein SYP-5. Crucially, canonical ultrastructure complex is altered upon weakening this interface. We developed thermodynamic model recapitulates our experimental observations, indicating liquid-like can assemble by wetting axes without active energy consumption. More broadly, data show condensation drives tightly regulated nuclear reorganization during sexual reproduction.
Language: Английский
Citations
2
Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11
Published: May 19, 2023
Plants are the source of our understanding several fundamental biological principles. It is well known that Gregor Mendel discovered laws Genetics in peas and maize was used for discovery transposons by Barbara McClintock. Plant models still useful general key concepts. In this article, we will focus on discussing recent plant studies have shed new light mysterious mechanisms meiotic crossover (CO) interference, heterochiasmy, obligatory CO, CO homeostasis. Obligatory necessary equilibrated segregation homologous chromosomes during meiosis. The tight control different male female rates (heterochiasmy) enables both maximization minimization genome shuffling. An integrative model can now predict these observed aspects patterning plants. mechanism proposed considers Synaptonemal Complex as a canalizing structure allows diffusion class I limiting factor linearly synapsed bivalents. coarsening along SC explains interfering spacing between COs. coordinated processes synapsis, insurance, also easily heterochiasmy just considering lengths. This expected to be conserved other species.
Language: Английский
Citations
6
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown
Published: Nov. 10, 2023
During meiosis, each pair of homologous chromosomes becomes connected by at least one crossover, as required for accurate segregation, and adjacent crossovers are widely separated thereby limiting total numbers. In coarsening models, this crossover patterning results from nascent recombination sites competing to accrue a pro-crossover RING-domain protein (COR) that diffuses between synapsed chromosomes. Here we delineate the localization dynamics three mammalian CORs in mouse determine their interdependencies. RNF212, HEI10 new member RNF212B show divergent spatiotemporal along chromosomes, including profound differences spermatocytes oocytes, not easily reconciled elementary models. Contrasting mutant phenotypes genetic requirements indicate RNF212B, play distinct but interdependent functions regulating meiotic coordinating events prophase-I integrating signals DNA breaks, homolog synapsis, cell-cycle, incipient sites.
Language: Английский
Citations
4
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 9, 2024
Abstract Crossovers create genetic diversity and are required for equal chromosome segregation during meiosis. Their number distribution highly regulated by different, yet not fully understood mechanisms including crossover interference. Crucial formation is the axis. Here, we explore function of axis protein ASY3. To this end, use allotetraploid species Brassica napus due to its polyploid nature, system allows a fine-grained dissection dosage meiotic regulators. The simultaneous mutation all four ASY3 alleles results in defective synapsis drastic reduction crossovers, which largely rescued presence only one functional allele. Crucially, while class I crossovers mutants with two comparable that wildtype, significantly increased allele, indicating reducing increases formation. Moreover, on each bivalent allele follow random distribution, compromised These reveal dosage-dependent distinct effects formation, provide insights into role patterning recombination.
Language: Английский
Citations
1
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: May 24, 2024
Abstract Meiotic chromosomes efficiently transduce information along their length to regulate the distribution of genetic exchanges within and between chromosomes. However, mode signal transduction remains unknown. Recently, a conserved chromosomal interface, synaptonemal complex, was shown be biomolecular condensate, offering an attractive mechanism for transduction: diffusion signaling molecules complex allow transmission each pair Here, we test feasibility this in live C. elegans gonads. Single-molecule tracking shows that component (SYP-3) regulator (ZHP-3) both diffuse complex. ZHP-3 diffuses 4- 9-fold faster than SYP-3 before after crossovers formation, respectively. We use these measurements parameterize physical model transduction. find ZHP-3, but not SYP-3, explores lengths on time scale crossover maturation, consistent with role spatial regulation exchanges. Given conservation paralogs across eukaryotes, propose may meiotic regulation. More broadly, our work how contained condensates regulates crucial cellular functions.
Language: Английский
Citations
1
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: Oct. 17, 2024
Crossover interference is a phenomenon that affects the number and positioning of crossovers in meiosis thus genetic diversity chromosome segregation. Yet, underlying mechanism not fully understood, partly because quantification difficult. To overcome this challenge, we introduce length L
Language: Английский
Citations
1
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 7, 2024
Abstract Meiotic crossovers are limited in number and unevenly distributed along chromosomes, both features often differing between sexes. The mechanisms imposing a different crossover landscape female male meiosis remain elusive. Here, we simultaneously disrupted multiple anti-crossover Arabidopsis analyzed the whole genome sequence of thousands female- male-derived progenies. largest increase was reached zyp1 recq4 , with 12-fold females 4.5-fold males. Despite this unprecedented level crossovers, fertility is marginally affected, opening new possibilities for plant breeding. Manipulating additional regulators did not further elevate frequency but modified relative contributions two known pathways. This suggests an upper limit pathways compete large set recombination intermediates. Remarkably, while wild-type distribution differs markedly sexes, landscapes diverse mutants males converge to single novel profile, which termed Crossover Potential (CO P ). CO defined using 49,482 can be accurately predicted only divergence chromatin features. We propose that represents density eligible precursors, determined by genomic thus identical It sexual dimorphism results exclusively from differential regulation likeness precursors mature into crossovers.
Language: Английский
Citations
1