Optical time-harmonic elastography for multiscale stiffness mapping across the phylogenetic tree DOI Creative Commons
Jakob Jordan,

Noah Jaitner,

Tom Meyer

et al.

arXiv (Cornell University), Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 1, 2023

Rapid mapping of the mechanical properties soft biological tissues from light microscopy to macroscopic imaging could transform fundamental biophysical research by providing clinical biomarkers complement in vivo elastography. We here introduce superfast optical time-harmonic elastography (OTHE) remotely encode surface and subsurface shear wave fields for generating maps tissue stiffness with unprecedented detail resolution. OTHE rigorously exploits space-time propagation characteristics waves address current limitations biomechanical Key solutions are presented stimulation, encoding, reconstruction time-harmonic, multifrequency waves, all tuned provide consistent values across resolutions microns millimeters. OTHE's versatility is demonstrated Bacillus subtilis biofilms, zebrafish embryos, adult zebrafish, human skeletal muscle, reflecting diversity phylogenetic tree a mechanics perspective. By zooming on details coarse finer scales, advances developmental biology offers way perform biomechanics-based histology that consistently matches patients.

Language: Английский

Vertical confinement enhances surface exploration in bacterial twitching motility DOI
Xiaohong Chen, Rongjing Zhang, Junhua Yuan

et al.

Environmental Microbiology, Journal Year: 2024, Volume and Issue: 26(7)

Published: July 1, 2024

Abstract Bacteria are often found in environments where space is limited, and they attach themselves to surfaces. One common form of movement on these surfaces bacterial twitching motility, which powered by the extension retraction type IV pili. Although motility unrestricted conditions has been extensively studied, effects spatial confinement this behaviour not well understood. In study, we explored diffusive properties individual Pseudomonas aeruginosa cells spatially confined conditions. We achieved placing bacteria between layers agarose glass, then tracking long‐term cells. Interestingly, that while reduced immediate speed twitching, it paradoxically increased diffusion. Through a combination mechanical geometrical analysis, as numerical simulations, showed increase diffusion could be attributed factors. The constraint imposed altered pattern from normal superdiffusion. These findings provide valuable insights into motile environments.

Language: Английский

Citations

2
Screening different solid supports for Pseudomonas aeruginosa biofilm formation and determining its efficiency for decolorization and degradation of congo red DOI

Pranati Das,

Anshita Mehra,

Shashwati Ghosh Sachan

et al.

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(10)

Published: Sept. 11, 2024

Language: Английский

Citations

1
Sulfolobus acidocaldariusadhesion pili power twitching motility in the absence of a dedicated retraction ATPase DOI Creative Commons
Arthur Charles‐Orszag, Marleen van Wolferen, Samuel J. Lord

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 4, 2023

Type IV pili are ancient and widespread filamentous organelles found in most bacterial archaeal phyla where they support a wide range of functions, including substrate adhesion, DNA uptake, self aggregation, cell motility. In bacteria, PilT-family ATPases disassemble adhesion pili, causing them to rapidly retract produce twitching motility, important for surface colonization. As archaea do not possess homologs PilT, it was thought that cannot retract. Here, we employ live-cell imaging under native conditions (75°C pH 2), together with automated single-cell tracking, high-temperature fluorescence imaging, genetic manipulation demonstrate

Language: Английский

Citations

3
Type-IV pili tune an adhesion-migration trade-off during surface colonization ofPseudomonas aeruginosa DOI Open Access
Ahmet Nihat Şimşek, Matthias D. Koch, Joseph E. Sanfilippo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 9, 2023

Bacterial pathogenicity relies on both firm surface adhesion and cell dissemination. How twitching bacteria resolve the fundamental contradiction between migration is unknown. To address this question, we employ live-cell imaging of type-IV pili (T4P) therewith construct a comprehensive mathematical model

Language: Английский

Citations

2
Optical time-harmonic elastography for multiscale stiffness mapping across the phylogenetic tree DOI Creative Commons
Jakob Jordan,

Noah Jaitner,

Tom Meyer

et al.

arXiv (Cornell University), Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 1, 2023

Rapid mapping of the mechanical properties soft biological tissues from light microscopy to macroscopic imaging could transform fundamental biophysical research by providing clinical biomarkers complement in vivo elastography. We here introduce superfast optical time-harmonic elastography (OTHE) remotely encode surface and subsurface shear wave fields for generating maps tissue stiffness with unprecedented detail resolution. OTHE rigorously exploits space-time propagation characteristics waves address current limitations biomechanical Key solutions are presented stimulation, encoding, reconstruction time-harmonic, multifrequency waves, all tuned provide consistent values across resolutions microns millimeters. OTHE's versatility is demonstrated Bacillus subtilis biofilms, zebrafish embryos, adult zebrafish, human skeletal muscle, reflecting diversity phylogenetic tree a mechanics perspective. By zooming on details coarse finer scales, advances developmental biology offers way perform biomechanics-based histology that consistently matches patients.

Language: Английский

Citations

1