Clock-dependent chromatin accessibility rhythms regulate circadian transcription DOI Creative Commons
Ye Yuan, Qianqian Chen, Margarita Brovkina

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 16, 2023

Abstract Chromatin organization plays a crucial role in gene regulation by controlling the accessibility of DNA to transcription machinery. While significant progress has been made understanding regulatory clock proteins circadian rhythms, how chromatin affects rhythms remains poorly understood. Here, we employed ATAC-seq (Assay for Transposase-Accessible with Sequencing) on FAC-sorted Drosophila neurons assess genome-wide over cycle. We observed oscillations at promoter and enhancer regions hundreds genes, enhanced either dusk or dawn, which correlated their peak transcriptional activity. Notably, genes were enriched E-box motifs, while those more accessible dawn VRI/PDP1-box indicating that they are regulated core feedback loops, PER/CLK VRI/PDP1, respectively. Further, complete loss per 01 null mutants, consistently throughout cycle, underscoring critical Period protein driving compaction during repression phase. Together, this study demonstrates regulation, revealing interplay between structure orchestrates precise timing biological processes day. This work further implies variations might play central generation diverse expression patterns neurons. Significance Statement development disease. In study, discovered robust elements clock-regulated neurons, found enrichment motifs exhibited dusk, higher dawn. Moreover, mutants highlights essential significance ∼24-hour rhythms.

Language: Английский

The glial UDP-glycosyltransferase Ugt35b regulates longevity by maintaining lipid homeostasis in Drosophila DOI Creative Commons
Lihong Sheng,

Jianpeng Gao,

Qingyuan Wei

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 44(1), P. 115099 - 115099

Published: Dec. 24, 2024

Language: Английский

Citations

0
Phylogenomics of the ecdysteroid kinase-like (EcKL) gene family in insects highlights roles in both steroid hormone metabolism and detoxification DOI Creative Commons
Jack L. Scanlan, Charles Robin

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 1, 2023

Abstract The evolutionary dynamics of large gene families can offer important insights into the functions their individual members. While ecdysteroid kinase-like (EcKL) family has previously been linked to metabolism both steroid moulting hormones and xenobiotic toxins, nearly all EcKL genes are unknown there is little information on evolution across insects. Here, we perform comprehensive phylogenetic analyses a manually annotated set from 140 insect genomes, revealing comprised at least 13 subfamilies that differ in retention stability. Our results show only two known encode kinases belong different therefore must be spread throughout family. We also provide comparative phylogenomic evidence EcKLs involved detoxification insects, with positive associations between size dietary chemical complexity, find similar for cytochrome P450 glutathione S-transferase families. Unexpectedly, clade containing kinase positively associated host plant taxonomic diversity Lepidoptera, possibly suggesting multiple functional shifts hormone metabolism. This work provides robust framework future studies opens promising new avenues exploring genomic basis adaptation including classically studied co-evolution butterflies plants. Significance statement inactivation but most its members unknown. study history showcase how phylogenetics inform characterisation enzyme varies over an order magnitude chemically complex diets, implicating numerous detoxification. surprising link steroid-metabolising moths, may evolve through insect-plant ‘chemical arms-race’. advances hypotheses clades proposes classification system this poorly characterised

Language: Английский

Citations

1
Stem cell-specific ecdysone signaling regulates the development and function of aDrosophilasleep homeostat DOI Creative Commons
Adil R. Wani, Budhaditya Chowdhury,

Jenny Luong

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 2, 2023

Abstract Complex behaviors arise from neural circuits that are assembled diverse cell types. Sleep is a conserved and essential behavior, yet little known regarding how the nervous system generates neuron types of sleep-wake circuit. Here, we focus on specification Drosophila sleep-promoting neurons—long-field tangential input neurons project to dorsal layers fan-shaped body neuropil in central complex (CX). We use lineage analysis genetic birth dating identify two bilateral Type II stem cells generate these (dFB) neurons. show adult dFB express Ecdysone-induced protein E93, loss Ecdysone signaling or E93 NSCs results misspecification Finally, knockdown affects sleep behavior. Our provide insight into extrinsic hormonal acts neuronal diversity required for These findings suggest some disorders might derive defects cell-specific temporal neurodevelopmental programs.

Language: Английский

Citations

1
Clock-dependent chromatin accessibility rhythms regulate circadian transcription DOI Creative Commons
Ye Yuan, Qianqian Chen, Margarita Brovkina

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 16, 2023

Abstract Chromatin organization plays a crucial role in gene regulation by controlling the accessibility of DNA to transcription machinery. While significant progress has been made understanding regulatory clock proteins circadian rhythms, how chromatin affects rhythms remains poorly understood. Here, we employed ATAC-seq (Assay for Transposase-Accessible with Sequencing) on FAC-sorted Drosophila neurons assess genome-wide over cycle. We observed oscillations at promoter and enhancer regions hundreds genes, enhanced either dusk or dawn, which correlated their peak transcriptional activity. Notably, genes were enriched E-box motifs, while those more accessible dawn VRI/PDP1-box indicating that they are regulated core feedback loops, PER/CLK VRI/PDP1, respectively. Further, complete loss per 01 null mutants, consistently throughout cycle, underscoring critical Period protein driving compaction during repression phase. Together, this study demonstrates regulation, revealing interplay between structure orchestrates precise timing biological processes day. This work further implies variations might play central generation diverse expression patterns neurons. Significance Statement development disease. In study, discovered robust elements clock-regulated neurons, found enrichment motifs exhibited dusk, higher dawn. Moreover, mutants highlights essential significance ∼24-hour rhythms.

Language: Английский

Citations

0