Extreme distributions in the preconfigured developing brain DOI Creative Commons
Mattia Chini,

Marilena Hnida,

Johanna K. Kostka

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 14, 2023

Abstract In the adult brain, structural and functional parameters, such as synaptic sizes neuronal firing rates, follow right-skewed heavy-tailed distributions. While this organization is thought of having significant implications, its development still largely unknown. Here, we address knowledge gap by investigating a large-scale dataset recorded from prefrontal cortex (PFC) olfactory bulb mice aged 4-60 postnatal days. We show that rates pairwise correlations have stable distribution shape over age, neural activity displays small-world architecture. Moreover, early brain an oligarchical organization, i.e., neurons with high are likely to hub-like properties. Leveraging network modeling, analogously extremely distributed parameters necessary recapitulate experimental data. Thus, in developing already distributed, suggesting preconfigured not experience-dependent.

Language: Английский

Principles of GABAergic signaling in developing cortical network dynamics DOI Creative Commons
Knut Kirmse, Chuanqiang Zhang

Cell Reports, Journal Year: 2022, Volume and Issue: 38(13), P. 110568 - 110568

Published: March 1, 2022

GABAergic signaling provides inhibitory stabilization and spatiotemporally coordinates the firing of recurrently connected excitatory neurons in mature cortical circuits. Inhibition thus enables self-generated neuronal activity patterns that underlie various aspects sensation cognition. In this review, we aim to provide a conceptual framework describing how when GABA-releasing interneurons acquire their network functions during development. Focusing on developing visual neocortex hippocampus mice rats vivo, hypothesize at onset patterned activity, glutamatergic are stable by themselves is not yet functional. We review important milestones development illustrate cell-type-specific strengthening synaptic inhibition toward eye opening shapes dynamics allows cortex progressively disengage from extra-cortical drive. translate human discuss clinical implications for treatment neonatal seizures.

Language: Английский

Citations

23
Multiphoton imaging of hippocampal neural circuits: techniques and biological insights into region-, cell-type-, and pathway-specific functions DOI Creative Commons
Kotaro Mizuta, Masaaki Sato

Neurophotonics, Journal Year: 2024, Volume and Issue: 11(03)

Published: March 8, 2024

SignificanceThe function of the hippocampus in behavior and cognition has long been studied primarily through electrophysiological recordings from freely moving rodents. However, application optical recording methods, particularly multiphoton fluorescence microscopy, last decade or two dramatically advanced our understanding hippocampal function. This article provides a comprehensive overview techniques biological findings obtained imaging neural circuits.AimThis review aims to summarize discuss recent technical advances circuits accumulated knowledge gained this technology.ApproachFirst, we provide brief various its advantages, drawbacks, associated key innovations practices. Then, large body by region (CA1 dentate gyrus), cell type (pyramidal neurons, inhibitory interneurons, glial cells), cellular compartment (dendrite axon).ResultsMultiphoton is performed under head-fixed conditions can reveal detailed mechanisms circuit operation owing high spatial resolution specificity. As lies deep below cortex, requires elaborate methods. These include cannula implantation, microendoscopy, use long-wavelength light sources. Although many studies have focused on dorsal CA1 pyramidal cells, other local inter-areal circuitry elements also helped more picture information processing circuits. Imaging mouse models Alzheimer's disease brain disorders such as autism spectrum disorder contributed greatly their pathophysiology.ConclusionsMultiphoton revealed much regarding region-, cell-type-, pathway-specific dysfunction health disease. Future technological will allow further illustration operating principle via large-scale, high-resolution, multimodal, minimally invasive imaging.

Language: Английский

Citations

5
Preconfigured architecture of the developing mouse brain DOI Creative Commons
Mattia Chini,

Marilena Hnida,

Johanna K. Kostka

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(6), P. 114267 - 114267

Published: May 24, 2024

In the adult brain, structural and functional parameters, such as synaptic sizes neuronal firing rates, follow right-skewed heavy-tailed distributions. While this organization is thought to have significant implications, its development still largely unknown. Here, we address knowledge gap by investigating a large-scale dataset recorded from prefrontal cortex olfactory bulb of mice aged 4–60 postnatal days. We show that rates spike train interactions stable distribution shape throughout first 60 days displays small-world architecture. Moreover, early brain activity exhibits an oligarchical organization, where high-firing neurons hub-like properties. neural network model, analogously parameters are instrumental consistently recapitulate experimental data. Thus, in developing already extremely distributed, suggesting preconfigured not experience dependent.

Language: Английский

Citations

4
Excitatory and inhibitory synapses show a tight subcellular correlation that weakens over development DOI Creative Commons

Sally Horton,

Vincenzo Mastrolia, Rachel E. Jackson

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(7), P. 114361 - 114361

Published: June 18, 2024

Neurons receive correlated levels of excitation and inhibition, a feature that is important for proper brain function. However, how this relationship between excitatory inhibitory inputs established during the dynamic period circuit wiring remains unexplored. Using multiple techniques, including in utero electroporation, electron microscopy, electrophysiology, we reveal tight correlation distribution synapses along dendrites developing CA1 hippocampal neurons. This was present within short dendritic stretches (<20 μm) and, surprisingly, most pronounced early development, sharply declining with maturity. The matching inhibition unexpected, as lacked an active zone when formed exhibited compromised evoked release. We propose form stabilizing scaffold to counterbalance growing levels. diminishes over time, suggesting critical role subcellular balance neuronal function formation.

Language: Английский

Citations

3
Network instability dynamics drive a transient bursting period in the developing hippocampus in vivo DOI Creative Commons
Jürgen Graf, Vahid Rahmati, Myrtill Majoros

et al.

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Dec. 19, 2022

Spontaneous correlated activity is a universal hallmark of immature neural circuits. However, the cellular dynamics and intrinsic mechanisms underlying network burstiness in intact developing brain are largely unknown. Here, we use two-photon Ca

Language: Английский

Citations

9
Chemogenetic silencing reveals presynaptic Gi/o protein-mediated inhibition of synchronized activity in the developing hippocampus in vivo DOI Creative Commons
Jürgen Graf, Arash Samiee, Tom Floßmann

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 2, 2024

The development of neuronal circuits is an activity-dependent process. Research aimed at deciphering the learning rules governing these developmental refinements increasingly utilizing a class chemogenetic tools that employ Gi/o protein signaling (Gi-DREADDs) for silencing. However, their mechanisms action and inhibitory efficacy in immature neurons with incompletely developed are poorly understood. Here, we analyze impact on cellular network excitability neonatal hippocampus by expressing Gi-DREADD hM4Di telencephalic glutamatergic mice both sexes. Using acousto-optic two-photon Ca2+ imaging, report activation leads to complete arrest spontaneous synchronized activity CA1 vitro. Electrophysiological analyses demonstrate hM4Di-mediated silencing not accounted changes intrinsic pyramidal cells (PCs). Instead, robustly restrains synaptic glutamate release first postnatal week, effectively reducing recurrent excitation CA1. In vivo, inhibition through potently suppresses early sharp waves (eSPWs) discontinuous oscillatory head-fixed before eye opening. summary, dampens neurotransmission presynaptic mechanism sufficient terminate Our findings have implications designing interpreting experiments Gi-DREADDs further point potential role Gi/o-dependent endogenous neuromodulators hippocampal development.

Language: Английский

Citations

0
Anti-NMDAR encephalitis alters intrinsic spatiotemporal coding by enhancing neuronal coupling and clustering DOI Creative Commons
Vahid Rahmati, Jürgen Graf, Mihai Ceangă

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 21, 2024

Summary Autoimmune anti-NMDA-receptor encephalitis is characterized by severe neuropsychiatric symptoms including memory dysfunction and seizures. However, it remains enigmatic what functional changes at the multi-neuronal level mediate network dysfunction. We used two-photon in vivo recording a passive-transfer mouse model with patient’s monoclonal anti-GluN1-autoantibodies during slow-wave sleep-like conditions, critical phase for processing. find enhanced coupling clustering between hippocampal CA1 pyramidal neurons (PNs), which intrinsically expose to hypersynchrony. These connectivity are associated selective preservation of strong excitatory synapses despite overall reduced excitation, thus enhancing hub-like properties functionally connected PNs. Furthermore, we abnormal PN firing characteristics, decreased transmission failure, increased similarity spontaneous spatiotemporal activity patterns, all affecting intrinsic neuronal coding. Collectively, rewiring networks altered information processing provide new mechanistic insights into NMDAR-hypofunction consequences pathomechanisms anti-NMDAR symptomatology.

Language: Английский

Citations

0
Chemogenetic silencing reveals presynaptic Gi/o protein-mediated inhibition of developing hippocampal synchrony in vivo DOI Creative Commons
Jürgen Graf, Arash Samiee, Tom Floßmann

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(10), P. 110997 - 110997

Published: Sept. 20, 2024

Language: Английский

Citations

0
Extreme distributions in the preconfigured developing brain DOI Creative Commons
Mattia Chini,

Marilena Hnida,

Johanna K. Kostka

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 14, 2023

Abstract In the adult brain, structural and functional parameters, such as synaptic sizes neuronal firing rates, follow right-skewed heavy-tailed distributions. While this organization is thought of having significant implications, its development still largely unknown. Here, we address knowledge gap by investigating a large-scale dataset recorded from prefrontal cortex (PFC) olfactory bulb mice aged 4-60 postnatal days. We show that rates pairwise correlations have stable distribution shape over age, neural activity displays small-world architecture. Moreover, early brain an oligarchical organization, i.e., neurons with high are likely to hub-like properties. Leveraging network modeling, analogously extremely distributed parameters necessary recapitulate experimental data. Thus, in developing already distributed, suggesting preconfigured not experience-dependent.

Language: Английский

Citations

0