Neural-circuit basis of song preference learning in fruit flies DOI Creative Commons

Keisuke Imoto,

Yuki Ishikawa, Yoshinori Aso

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 26, 2023

Abstract As observed in human language learning and song birds, the fruit fly Drosophila melanogaster changes its’ auditory behaviors according to prior sound experiences. Female flies that have heard male courtship songs of same species are less responsive different species. This phenomenon, known as preference flies, requires GABAergic input pC1 neurons central brain, with these playing a key role mating behavior by integrating multimodal sensory internal information. The neural circuit basis this input, however, has not yet been identified. Here, we find pCd-2 neurons, totaling four cells per hemibrain expressing sex-determination gene doublesex , provide for learning. First, RNAi-mediated knockdown GABA production abolished Second, directly, many cases mutually, connect suggesting existence reciprocal circuits between neurons. Finally, dopaminergic inputs necessary Together, study suggests serve state-integrated hub, allowing flexible control over female copulation. Consequently, provides model underlies experience-dependent plasticity. Significance To suitable mate, an organism must adapt its based on past In case assessments signals, which contain information about status sender, experience dependent. show brain modulate female’s response depending her previous These exhibit feedback lateral inhibition motifs, regulated inputs. While effects experiences responsiveness extensively studied other species, our research advances use dissecting circuitry underlying plasticity at single-cell resolution.

Language: Английский

The Oviposition Inhibitory Neuron is a potential hub of multi-circuit integration in the Drosophila brain DOI Open Access

Rhessa Weber Langstaff,

Pranjal Srivastava, Alexander B. Kunin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 26, 2024

Abstract Understanding how neural circuits integrate sensory and state information to support context-dependent behavior is a central issue in neuroscience. In Drosophila, oviposition complex process which the fly integrates context choose an optimal location lay her eggs. The circuit that controls sequence known, but multiple modalities internal states not. We investigated circuitry underlying high-level processing related using Hemibrain connectome. identified Oviposition Inhibitory Neuron (oviIN) as key hub analyzed its inputs uncover potential parallel pathways may be responsible for computations decision-making. applied graph-theoretic analyses on sub-connectome of oviIN identify modules neurons constitute novel circuits. Our findings indicate form from unstructured neuropils Superior Protocerebrum where have been known occur.

Language: Английский

Citations

0
Insulin/insulin-like growth factor signaling pathway promotes higher fat storage inDrosophilafemales DOI Creative Commons
Puja Biswas, Huaxu Yu, Lianna W. Wat

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

Summary In Drosophila , adult females store more fat than males. While the mechanisms that restrict body in males are becoming clearer, less is known about how achieve higher storage. Here, we perform a detailed investigation of promote storage females. We show greater intake dietary sugar supports due to female-biased remodeling lipidome. Dietary stimulates female-specific increase insulin-like peptide 3 (Dilp3), which acts together with peripheral insulin sensitivity augment insulin/insulin-like growth factor signaling pathway (IIS) activity Given adult-specific IIS inhibition reduced sex difference decrease fat, our data reveal as key determinant female

Language: Английский

Citations

0
Insulin/Insulin-Like Growth Factor Signaling Pathway Promotes Higher Fat Storage in Drosophila Females DOI
Puja Biswas, Huaxu Yu, Lianna W. Wat

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Hunger- and thirst-sensing neurons modulate a neuroendocrine network to coordinate sugar and water ingestion DOI Creative Commons

Amanda J. González-Segarra,

Gina Pontes, Nicholas Jourjine

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 8, 2023

ABSTRACT Consumption of food and water is tightly regulated by the nervous system to maintain internal nutrient homeostasis. Although generally considered independently, interactions between hunger thirst drives are important coordinate competing needs. In Drosophila , four neurons called Interoceptive Subesophageal zone Neurons (ISNs) respond intrinsic signals oppositely regulate sucrose ingestion. Here, we investigate neural circuit downstream ISNs examine how ingestion based on Utilizing recently available fly brain connectome, find that synapse with a novel cell type Bilateral T-shaped neuron (BiT) projects neuroendocrine centers. vivo manipulations revealed BiT regulates sugar Neuroendocrine cells include several peptide-releasing peptide-sensing neurons, including insulin producing (IPC), crustacean cardioactive peptide (CCAP) CCHamide-2 receptor isoform RA (CCHa2R-RA) neurons. These contribute differentially water, IPCs CCAP regulating ingestion, CCHa2R-RA modulating only Thus, decision consume or occurs via regulation broad peptidergic network integrates nutritional state generate nutrient-specific

Language: Английский

Citations

0
Neural-circuit basis of song preference learning in fruit flies DOI Creative Commons

Keisuke Imoto,

Yuki Ishikawa, Yoshinori Aso

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 26, 2023

Abstract As observed in human language learning and song birds, the fruit fly Drosophila melanogaster changes its’ auditory behaviors according to prior sound experiences. Female flies that have heard male courtship songs of same species are less responsive different species. This phenomenon, known as preference flies, requires GABAergic input pC1 neurons central brain, with these playing a key role mating behavior by integrating multimodal sensory internal information. The neural circuit basis this input, however, has not yet been identified. Here, we find pCd-2 neurons, totaling four cells per hemibrain expressing sex-determination gene doublesex , provide for learning. First, RNAi-mediated knockdown GABA production abolished Second, directly, many cases mutually, connect suggesting existence reciprocal circuits between neurons. Finally, dopaminergic inputs necessary Together, study suggests serve state-integrated hub, allowing flexible control over female copulation. Consequently, provides model underlies experience-dependent plasticity. Significance To suitable mate, an organism must adapt its based on past In case assessments signals, which contain information about status sender, experience dependent. show brain modulate female’s response depending her previous These exhibit feedback lateral inhibition motifs, regulated inputs. While effects experiences responsiveness extensively studied other species, our research advances use dissecting circuitry underlying plasticity at single-cell resolution.

Language: Английский

Citations

0