A split-GAL4 driver line resource for Drosophila CNS cell types DOI Creative Commons
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: July 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal cell-type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Language: Английский

Hierarchical architecture of dopaminergic circuits enables second-order conditioning in Drosophila DOI Creative Commons
Daichi Yamada, Daniel Bushey, Feng Li

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Jan. 24, 2023

Dopaminergic neurons with distinct projection patterns and physiological properties compose memory subsystems in a brain. However, it is poorly understood whether or how they interact during complex learning. Here, we identify feedforward circuit formed between dopamine show that essential for second-order conditioning, an ethologically important form of higher-order associative The Drosophila mushroom body comprises series dopaminergic compartments, each which exhibits dynamics. We find slow stable compartment can serve as effective ‘teacher’ by instructing other faster transient compartments via single key interneuron, connectome analysis neurotransmitter prediction. This excitatory interneuron acquires enhanced response to reward-predicting odor after first-order conditioning and, upon activation, evokes release the ‘student’ compartments. These hierarchical connections explain first- long known behavioral psychologists.

Language: Английский

Citations

43
New genetic tools for mushroom body output neurons in Drosophila DOI Creative Commons
Gerald M. Rubin, Yoshinori Aso

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Sept. 1, 2023

How memories of past events influence behavior is a key question in neuroscience. The major associative learning center Drosophila, the mushroom body (MB), communicates to rest brain through output neurons (MBONs). While 21 MBON cell types have their dendrites confined small compartments MB lobes, analysis EM connectomes revealed presence an additional 14 that are atypical having dendritic input both within lobes and adjacent regions. Genetic reagents for manipulating MBONs experimental data on functions been lacking. In this report we describe new cell-type-specific GAL4 drivers many MBONs, including majority extend collection driver lines previously generated (Aso et al., 2014a; Aso 2016; 2019). Using these genetic reagents, conducted optogenetic activation screening examine ability drive behaviors learning. These provide important tools study complex Drosophila.

Language: Английский

Citations

23
A split-GAL4 driver line resource forDrosophilaneuron types DOI Creative Commons
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 10, 2024

Abstract Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Language: Английский

Citations

12
A split-GAL4 driver line resource for Drosophila neuron types DOI Creative Commons
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 24, 2025

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal cell-type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Language: Английский

Citations

1
Driver lines for studying associative learning in Drosophila DOI Creative Commons
Yichun Shuai, Megan Sammons, Gabriella R Sterne

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 29, 2025

The mushroom body (MB) is the center for associative learning in insects. In Drosophila , intersectional split-GAL4 drivers and electron microscopy (EM) connectomes have laid foundation precise interrogation of MB neural circuits. However, investigation many cell types upstream downstream has been hindered due to lack specific driver lines. Here we describe a new collection over 800 split-LexA that cover approximately 300 types, including sugar sensory neurons, putative nociceptive ascending olfactory thermo-/hygro-sensory projection interneurons connected with MB-extrinsic various other types. We characterized activation phenotypes subset these lines identified neuron line most suitable reward substitution. Leveraging thousands confocal images associated collection, analyzed neuronal morphological stereotypy discovered one set output MBON08/MBON09, exhibits striking individuality asymmetry across animals. conjunction EM connectome maps, reported here offer powerful resource functional dissection circuits adult .

Language: Английский

Citations

1
Driver lines for studying associative learning in Drosophila DOI Creative Commons
Yichun Shuai, Megan Sammons, Gabriella R Sterne

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 16, 2023

The mushroom body (MB) is the center for associative learning in insects. In Drosophila, intersectional split-GAL4 drivers and electron microscopy (EM) connectomes have laid foundation precise interrogation of MB neural circuits. However, investigation many cell types upstream downstream has been hindered due to lack specific driver lines. Here we describe a new collection over 800 split-LexA that cover approximately 300 types, including sugar sensory neurons, putative nociceptive ascending olfactory thermo-/hygro-sensory projection interneurons connected with MB-extrinsic various other types. We characterized activation phenotypes subset these lines identified neuron line most suitable reward substitution. Leveraging thousands confocal images associated collection, analyzed neuronal morphological stereotypy discovered one set output MBON08/MBON09, exhibits striking individuality asymmetry across animals. conjunction EM connectome maps, reported here offer powerful resource functional dissection circuits adult Drosophila.

Language: Английский

Citations

15
Driver lines for studying associative learning in Drosophila DOI Open Access
Yichun Shuai, Megan Sammons, Gabriella R Sterne

et al.

Published: Feb. 8, 2024

The mushroom body (MB) is the center for associative learning in insects. In Drosophila, intersectional split-GAL4 drivers and electron microscopy (EM) connectomes have laid foundation precise interrogation of MB neural circuits. However, investigation many cell types upstream downstream has been hindered due to lack specific driver lines. Here we describe a new collection over 800 split-LexA that cover approximately 300 types, including sugar sensory neurons, putative nociceptive ascending olfactory thermo-/hygro-sensory projection interneurons connected with MB-extrinsic various other types. We characterized activation phenotypes subset these lines identified neuron line most suitable reward substitution. Leveraging thousands confocal images associated collection, analyzed neuronal morphological stereotypy discovered one set output MBON08/MBON09, exhibits striking individuality asymmetry across animals. conjunction EM connectome maps, reported here offer powerful resource functional dissection circuits adult Drosophila.

Language: Английский

Citations

5
eLife assessment: A split-GAL4 driver line resource for Drosophila CNS cell types DOI Open Access
Tânia Reis

Published: July 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. In addition stocks for well-characterized lines, we make available 300,000 new 3D lines.

Language: Английский

Citations

4
A split-GAL4 driver line resource for Drosophila neuron types DOI Open Access
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

Published: Jan. 6, 2025

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Language: Английский

Citations

0
Driver lines for studying associative learning in Drosophila DOI Open Access
Yichun Shuai, Megan Sammons, Gabriella R Sterne

et al.

Published: Jan. 6, 2025

The mushroom body (MB) is the center for associative learning in insects. In Drosophila , intersectional split-GAL4 drivers and electron microscopy (EM) connectomes have laid foundation precise interrogation of MB neural circuits. However, investigation many cell types upstream downstream has been hindered due to lack specific driver lines. Here we describe a new collection over 800 split-LexA that cover approximately 300 types, including sugar sensory neurons, putative nociceptive ascending olfactory thermo-/hygro-sensory projection interneurons connected with MB-extrinsic various other types. We characterized activation phenotypes subset these lines identified neuron line most suitable reward substitution. Leveraging thousands confocal images associated collection, analyzed neuronal morphological stereotypy discovered one set output MBON08/MBON09, exhibits striking individuality asymmetry across animals. conjunction EM connectome maps, reported here offer powerful resource functional dissection circuits adult .

Language: Английский

Citations

0