Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts DOI Creative Commons
Masanori Izumi, Sakuya Nakamura, Kohei Otomo

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Dec. 19, 2023

Plants distribute many nutrients to chloroplasts during leaf development and maturation. When leaves senesce or experience sugar starvation, the autophagy machinery degrades chloroplast proteins facilitate efficient nutrient reuse. Here, we report on intracellular dynamics of an pathway responsible for piecemeal degradation components. Through live-cell monitoring morphology, observed formation budding structures in sugar-starved leaves. These buds were then released incorporated into vacuolar lumen as autophagic cargo termed a Rubisco-containing body. The did not accumulate mutants core machinery, suggesting that autophagosome creation is required forming buds. Simultaneous tracking morphology revealed isolation membranes autophagosomes interact closely with part surface before Chloroplasts protrude at site associated membranes, which divide synchronously This autophagy-related division does require DYNAMIN-RELATED PROTEIN 5B, constitutes ring proliferation growing An unidentified may thus fragment coordination chloroplast-associated membrane.

Language: Английский

A synthetic chlorophagy receptor promotes plant fitness by mediating chloroplast microautophagy DOI Creative Commons
Rui Liu, Xun Weng,

Xinjing Li

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Abstract Chloroplasts are major photosynthetic and protein-containing organelles in green plants algae. Unwanted chloroplast proteins entire chloroplasts cleared through various degradation pathways including autophagy. Nevertheless, canonical chlorophagy receptors remain unidentified, whether to what extent can be enhanced benefect the unknown. Here we designed validated a synthetic autophagy receptor using biochemical, genetical, imaging approaches. This receptor, LIR-SNT-BFP, was constructed by fusing fragment containing LC3-interacting region (LIR) of selective NBR1 N-terminal amphipathic α-helix outer envelope protein SFR2. The fusion LIR-SNT-BFP coated attracted ATG8a planta . Upon induction, elicited vacuole-mediated microautophagy independent ATG8 conjugation machinery ATG5 or ATG7. Meanwhile, it induced division; however PDV2. Notably, moderate improves rosette growth, but excessive levels detrimental. Furthermore, partially protects against herbicide-induced leaf chlorosis. study demonstrates controlled receptor.

Language: Английский

Citations

0
Light-induced CsCV triggers chloroplast degradation by destabilizing photosystem proteins in tea plant DOI
Xin Zhang,

Xiaobei Huang,

Zhijun Wu

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: 224, P. 109926 - 109926

Published: April 17, 2025

Language: Английский

Citations

0
SnRK1 and TOR: central regulators of autophagy in plant energy stress responses DOI Creative Commons
Lei Feng, Xibao Li,

Xuanang Zheng

et al.

aBIOTECH, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

Language: Английский

Citations

0
The burning glass effect of water droplets triggers a high light-induced calcium response in the chloroplast stroma DOI Creative Commons

Dominic Kuang,

Shanna Romand, Anna S. Zvereva

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Picky eaters: selective autophagy in plant cells DOI

Alexis I. Cadena‐Ramos,

Clelia De‐la‐Peña

The Plant Journal, Journal Year: 2023, Volume and Issue: 117(2), P. 364 - 384

Published: Oct. 21, 2023

SUMMARY Autophagy, a fundamental cellular process, plays vital role in maintaining homeostasis by degrading damaged or unnecessary components. While selective autophagy has been extensively studied animal cells, its significance plant cells only recently gained attention. In this review, we delve into the intriguing realm plants, with specific focus on involvement nutrient recycling, organelle turnover, and stress response. Moreover, recent studies have unveiled interesting interplay between epigenetic mechanisms elucidating of regulation modulating autophagy‐related gene expression finely tuning process plants. By synthesizing existing knowledge, review highlights emerging field emphasizing pivotal homeostasis, facilitating adaptation, shedding light that governs these processes. Our comprehensive study provides way for deeper understanding dynamic control responses to availability conditions, opening new avenues future research physiology.

Language: Английский

Citations

7
Full-length transcriptome sequencing of Arabidopsis plants provided new insights into the autophagic regulation of photosynthesis DOI Creative Commons
Song Wang, Yunfeng Shi, Yanhui Zhou

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: June 25, 2024

Abstract Autophagy is a highly conserved eukaryotic pathway and plays crucial role in cell survival under stress conditions. Here, we applied full-length transcriptome approach to study an Arabidopsis autophagy mutant ( atg5-1 ) subjected nitrogen-starvation, using Oxford Nanopore Technologies. A total of 39,033 transcripts were identified, including 11,356 new transcripts. In addition, alternative splicing (AS) events lncRNAs also detected between Col-0 (WT) . Differentially expressed transcript enrichment showed that upregulates the expression many stress-responsive genes inhibits transcription photosynthesis-associated genes. The qRT-PCR results patterns photosynthesis-related differed conditions nitrogen starvation carbon starvation. Under treatment, related photosynthesis exhibited AS. Chlorophyll fluorescence images revealed Fv/Fm ΦPSII old leaves significantly reduced after but Y NPQ indices increased compared those WT plants. suggest appears be involved degradation photodamage repair PSII. Taken together, transcriptiome sequencing provide insights into how transcripts, are plant through potential link photosynthesis.

Language: Английский

Citations

1
Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts DOI Creative Commons
Masanori Izumi, Sakuya Nakamura, Kohei Otomo

et al.

eLife, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 7, 2024

Plants distribute many nutrients to chloroplasts during leaf development and maturation. When leaves senesce or experience sugar starvation, the autophagy machinery degrades chloroplast proteins facilitate efficient nutrient reuse. Here, we report on intracellular dynamics of an pathway responsible for piecemeal degradation components. Through live-cell monitoring morphology, observed formation budding structures in sugar-starved leaves. These buds were then released incorporated into vacuolar lumen as autophagic cargo termed a Rubisco-containing body. The did not accumulate mutants core machinery, suggesting that autophagosome creation is required forming buds. Simultaneous tracking morphology revealed isolation membranes autophagosomes interact closely with part surface before Chloroplasts protrude at site associated membranes, which divide synchronously This autophagy-related division does require DYNAMIN-RELATED PROTEIN 5B, constitutes ring proliferation growing An unidentified may thus fragment coordination chloroplast-associated membrane.

Language: Английский

Citations

1
Chloroplast microautophagy: A green role for NBR1 DOI
Han Nim Lee, S. Marathe, Marisa S. Otegui

et al.

Autophagy, Journal Year: 2023, Volume and Issue: 19(12), P. 3244 - 3245

Published: Aug. 10, 2023

"Chloroplast microautophagy: A green role for NBR1." Autophagy, ahead-of-print(ahead-of-print), pp. 1–2 AcknowledgementsThis work was supported, in part, by grants from the U.S. National Science Foundation IOS-1840687 and Department of Energy grant DE-SC0019013.Disclosure StatementNo potential conflict interest reported author(s).Additional informationFundingThe supported [IOS-1840687]; [DE-SC0019013].

Language: Английский

Citations

1
Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts DOI Creative Commons
Masanori Izumi, Sakuya Nakamura, Kohei Otomo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 14, 2023

Abstract Plants distribute many nutrients to chloroplasts during leaf development and maturation. When leaves senesce or experience sugar starvation, the autophagy machinery degrades chloroplast proteins facilitate efficient nutrient reuse. Here, we report on intracellular dynamics of an pathway responsible for piecemeal degradation components. Through live-cell monitoring morphology, observed formation budding structures in sugar-starved leaves. These buds were then released incorporated into vacuolar lumen as autophagic cargo termed a Rubisco-containing body. The did not accumulate mutants core machinery, suggesting that autophagosome creation is required forming buds. Simultaneous tracking morphology revealed isolation membranes autophagosomes interact closely with part surface before Chloroplasts protrude at site associated membranes, which divide synchronously This autophagy-related division does require DYNAMIN-RELATED PROTEIN 5B, constitutes ring proliferation growing An unidentified may thus fragment coordination chloroplast-associated membrane.

Language: Английский

Citations

1
Genotype-Specific Activation of Autophagy during Heat Wave in Wheat DOI Creative Commons
Kathleen Hickey, Yunus Şahin,

Glenn W. Turner

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(14), P. 1226 - 1226

Published: July 20, 2024

Recycling of unnecessary or dysfunctional cellular structures through autophagy plays a critical role in homeostasis and environmental resilience. Therefore, the trait may have been unintentionally selected wheat breeding programs for higher yields arid climates. This hypothesis was tested by measuring response three common markers, ATG7, ATG8, NBR1, to heat wave under reduced soil moisture content 16 genetically diverse spring landraces originating from different geographical locations. We observed greenhouse trials that ATG8 NBR1 exhibited genotype-specific responses 1 h, 40 °C wave, while ATG7 did not show consistent response. Three genotypes Uruguay, Mozambique, Afghanistan showed pattern with autophagic activity: decreased stable abundance both proteins, coupled increased transcription NBR1. In contrast, Pakistan, Ethiopia, Egypt elevated protein levels alongside unaltered transcript levels, indicating potential suppression no change activity. Principal component analysis demonstrated correlation between lower proteins yield field trials. found (i) combination drought activated only several genotypes, suggesting despite being resilience mechanism, is heat-sensitive process; (ii) activity correlates positively greater yield; (iii) lack some high-yielding suggests contribution alternative stress-resilient mechanisms; (iv) enhanced independently geographic

Language: Английский

Citations

0