Oscillatory dynamics of Rac1 activity in Dictyostelium discoideum amoebae DOI Creative Commons

Marko Šoštar,

Maja Marinović, Vedrana Filić

et al.

PLoS Computational Biology, Journal Year: 2024, Volume and Issue: 20(12), P. e1012025 - e1012025

Published: Dec. 9, 2024

Small GTPases of the Rho family play a central role in regulation cell motility by controlling remodeling actin cytoskeleton. In amoeboid cells Dictyostelium discoideum, active form GTPase Rac1 regulates polymerases at leading edge and filament bundling proteins posterior cortex polarized cells. We monitored spatiotemporal dynamics its effector DGAP1 vegetative amoebae using specific fluorescent probes. observed that plasma membrane domains enriched not only exhibited stable polarization, but also showed rotations oscillations, whereas was depleted from these regions. To simulate two proteins, we developed mass-conserving reaction-diffusion model based on circulation between cytoplasm coupled with activation GEFs, deactivation GAPs interaction DGAP1. Our theoretical accurately reproduced experimentally dynamic patterns, including predominant anti-correlation Significantly, predicted new colocalization regime cells, which confirmed experimentally. summary, our results improve understanding reveal how occurrence transitions different regimes depend biochemical reaction rates, protein levels size. This study expands knowledge behavior D. discoideum demonstrates modes lead to their counterintuitively anti-correlated dynamics.

Language: Английский

Patterning of the cell cortex by Rho GTPases DOI
William M. Bement, Andrew B. Goryachev, Ann L. Miller

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2024, Volume and Issue: 25(4), P. 290 - 308

Published: Jan. 3, 2024

Language: Английский

Citations

50
Biomechanics of parasite migration within hosts DOI
Yi‐Ting Yeh, Juan C. del Álamo, Conor R. Caffrey

et al.

Trends in Parasitology, Journal Year: 2024, Volume and Issue: 40(2), P. 164 - 175

Published: Jan. 2, 2024

Language: Английский

Citations

5
Synchronization in collectively moving inanimate and living active matter DOI Creative Commons
Michael Riedl,

Isabelle Mayer,

Jack Merrin

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Sept. 13, 2023

Whether one considers swarming insects, flocking birds, or bacterial colonies, collective motion arises from the coordination of individuals and entails adjustment their respective velocities. In particular, in close confinements, such as those encountered by dense cell populations during development regeneration, migration can only arise coordinately. Yet, how unify velocities is often not understood. Focusing on a finite number cells circular we identify waves polymerizing actin that function pacemaker governing speed individual cells. We show onset coincides with synchronization wave nucleation frequencies across population. Employing simpler more readily accessible mechanical model system active spheres, individuals' internal oscillators essential requirements to reach corresponding state. The 'toy' experiment illustrates global synchronous state achieved nearest neighbor coupling. suggest analogy local coupling are for emergent, self-organized collectives.

Language: Английский

Citations

13
Exploring the Synthesis of Self-Organization and Active Motion DOI
Wen Zhu, Pamela Knoll, Oliver Steinbock

et al.

The Journal of Physical Chemistry Letters, Journal Year: 2024, Volume and Issue: 15(20), P. 5476 - 5487

Published: May 15, 2024

Proteins, genetic material, and membranes are fundamental to all known organisms, yet these components alone do not constitute life. Life emerges from the dynamic processes of self-organization, assembly, active motion, suggesting existence similar artificial systems. Against this backdrop, our Perspective explores a variety chemical phenomena illustrating how nonequilibrium self-organization micromotors contribute life-like behavior functionalities. After explaining key terms, we discuss specific examples including enzymatic diffusiophoretic bubble-driven self-propulsion, pattern-forming reaction-diffusion systems, self-assembling inorganic aggregates, hierarchically emergent phenomena. We also provide roadmap for combining motion possible outcomes through biological analogs. suggest that research direction, deeply rooted in physical chemistry, offers opportunities further development with broad impacts on related sciences technologies.

Language: Английский

Citations

4
Excitable Ras dynamics-based screens reveal RasGEFX is required for macropinocytosis and random cell migration DOI Creative Commons
K. Iwamoto, Satomi Matsuoka, Masahiro Ueda

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 2, 2025

Excitable systems of eukaryotic chemotaxis can generate asymmetric signals Ras-GTP-enriched domains spontaneously to drive random cell migration without guidance cues. However, the molecules responsible for spontaneous signal generation remain elusive. Here, we characterized RasGEFs encoded in Dictyostelium discoideum by live-cell imaging spatiotemporal dynamics Ras-GTP and hierarchical clustering, finding that RasGEFX is primarily required essential combination with RasGEFB/M/U starved cells, they are dispensable chemoattractant cAMP. RasGEFB co-localize regulate temporal periods spatial sizes oscillatory waves propagating along membrane, respectively, thus control protrusions motile cells differently, while RasGEFU RasGEFM adhesion speed, respectively. Remarkably, also important Ras/PIP3-driven macropinocytosis proliferating but not. These findings illustrate a specific coordinated cytoskeletal multiple motility macropinocytosis. Live-cell-imaging-based screens identify as an component pathway triggers Ras excitation basal macropinocytic cup formation fluid uptake.

Language: Английский

Citations

0
Spatiotemporal pattern formation of membranes induced by surface molecular binding/unbinding DOI Creative Commons
Hiroshi Noguchi

Soft Matter, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Nonequilibrium membrane pattern formation is studied using meshless simulation. We consider that molecules bind to either surface of a bilayer and move the opposite leaflet by flip-flop. When binding does not modify properties transfer rates among three states are cyclically symmetric, exhibits spiral-wave homogeneous-cycling modes at high low rates, respectively, as in an off-lattice cyclic Potts model. changes spontaneous curvature, these spatiotemporal dynamics coupled with microphase separation. two symmetric surfaces thermal equilibrium, domains form 4.8.8 tiling patterns addition stripe spot patterns. In nonequilibrium conditions, moving biphasic time-irreversible fluctuating appear. The ballistically or diffusively depending on conditions.

Language: Английский

Citations

0
Rho GTPase dynamics distinguish between models of cortical excitability DOI
Dominic A. Chomchai, Marcin Leda, Adriana E. Golding

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Role of Noise-Modulated Self-Propulsion in Driving Spatiotemporal Orders in Active Systems DOI
Kaustav Mondal, Tarpan Maiti, Pushpita Ghosh

et al.

Journal of Chemical Theory and Computation, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Fluctuations play a pivotal role in driving spatiotemporal order active matter systems. In this study, we employ novel analytical framework to investigate the impact of dichotomous noise on self-propelling velocity particle systems such as polymerizing actin filaments or reproducing elongated bacteria. By incorporating fluctuations with Ornstein-Zernike correlations into continuum-based model, derive bifurcation condition parameter space, revealing noise-induced instability that drives emergence traveling waves. This approach demonstrates how specific strengths and correlation times expand region by introducing effective new degrees freedom alter system's stability matrix. Advance numerical simulations, meticulously designed handle properties noise, validate these theoretical predictions reveal excellent agreement. A key finding is observation wave-reversal behavior, driven sign alternation noise-modulated advection term modulated relaxation time. Remarkably, identify finite range where reversal suppressed, offering insights bifurcations dynamics. Our combined provides deeper understanding shaping self-organization pattern formation biological synthetic

Language: Английский

Citations

0
Nonequilibrium Membrane Dynamics Induced by Active Protein Interactions and Chemical Reactions: A Review DOI Creative Commons
Hiroshi Noguchi

ChemSystemsChem, Journal Year: 2024, Volume and Issue: unknown

Published: July 22, 2024

Abstract Biomembranes wrapping cells and organelles are not only the partitions that separate insides but also dynamic fields for biological functions accompanied by membrane shape changes. In this review, we discuss spatiotemporal patterns fluctuations of membranes under nonequilibrium conditions. particular, focus on theoretical analyses simulations. Protein active forces enhance or suppress fluctuations; height spectra deviated from thermal spectra. binding unbinding to is activated inhibited other proteins chemical reactions, such as ATP hydrolysis. Such processes can induce traveling waves, Turing patterns, morphological They be represented continuum reaction‐diffusion equations discrete lattice/particle models with state flips. The effects structural changes in amphiphilic molecules molecular‐assembly structures discussed.

Language: Английский

Citations

3
Solution of steady state in the model polymer system with rupture and rebinding DOI
Prakhar Shukla, Neha Pathak, Pallavi Debnath

et al.

Physica Scripta, Journal Year: 2024, Volume and Issue: 99(3), P. 035946 - 035946

Published: Feb. 7, 2024

Abstract In this paper, we study the steady state attained in our model polymer system that attempts to explain relative motion between soft rubbing surfaces at single level. We generalize one-dimensional [Borah et al , 2016 Soft Matter 12 4406] by including rebinding of interconnecting bonds a flexible transducer (bead spring polymer) and rigid fixed plate. The described as harmonic springs rupture rebind stochastically when constant force pulls transducer. obtain distinct stochastic simulations bead positions bond states (closed or open) are independent time, analogous creep frictional systems termination earthquakes. simulation results for specific parameter sets agree with numerical solution mean-field equations developed analytical tractability. develop an within homotopy analysis method, which converges agrees well results.

Language: Английский

Citations

2