Assembly of a functional neuronal circuit in embryos of an ancestral metazoan is influenced by environmental signals including the microbiome DOI
Christopher Noack, Sebastian Jenderny, Christoph Giez

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 15, 2024

Abstract Understanding how neural populations evolve to give rise behavior is a major goal in neuroscience. However, the complexity of nervous system most invertebrates and vertebrates complicates deciphering underlying fundamental processes. Here, we explore self-assembly circuits Hydra , an organism with simple but no centralized information processing, improve understanding evolution. The N4 neuronal circuit embryos develops through activity-driven self-assembly, where neurons distinct regions increase connectivity synchronization. Gap junctions vesicle-mediated communication between non-neuronal cells drive rapid assembly, embryo’s prospective oral region exhibiting highest density. An artificial electrical circuit-based model demonstrates dynamic increases synchronization over time, along predictions for selective adaptions connections. Environmental factors, like temperature absent microbiome, modify architecture, suggesting existence certain plasticity development. We propose that these features originated last common bilaterian ancestor, supporting hypothesis basic architecture universal.

Language: Английский

Differentiation trajectories of theHydranervous system reveal transcriptional regulators of neuronal fate DOI Creative Commons
Abby S. Primack, Jack F. Cazet, Hannah Morris Little

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: March 16, 2023

The small freshwater cnidarian polyp

Language: Английский

Citations

14
Automatic monitoring of neural activity with single-cell resolution in behaving Hydra DOI Creative Commons
Alison Hanson,

Raphael Reme,

Noah Telerman

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 1, 2024

The ability to record every spike from neuron in a behaving animal is one of the holy grails neuroscience. Here, we report coming step closer towards this goal with development an end-to-end pipeline that automatically tracks and extracts calcium signals individual neurons cnidarian Hydra vulgaris. We imaged dually labeled (nuclear tdTomato cytoplasmic GCaMP7s) transgenic developed open-source Python platform (TraSE-IN) for Tracking Spike Estimation Individual Neurons during behavior. TraSE-IN comprises series modules segments each nucleus over time corresponding activity GCaMP channel. Another signal processing allows robust prediction spikes neuron's signal. This complete will facilitate automatic generation analysis large-scale datasets single-cell resolution neural Hydra, potentially other model organisms, paving way deciphering code entire animal.

Language: Английский

Citations

1
Assembly of a functional neuronal circuit in embryos of an ancestral metazoan is influenced by environmental signals including the microbiome DOI
Christopher Noack, Sebastian Jenderny, Christoph Giez

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 15, 2024

Abstract Understanding how neural populations evolve to give rise behavior is a major goal in neuroscience. However, the complexity of nervous system most invertebrates and vertebrates complicates deciphering underlying fundamental processes. Here, we explore self-assembly circuits Hydra , an organism with simple but no centralized information processing, improve understanding evolution. The N4 neuronal circuit embryos develops through activity-driven self-assembly, where neurons distinct regions increase connectivity synchronization. Gap junctions vesicle-mediated communication between non-neuronal cells drive rapid assembly, embryo’s prospective oral region exhibiting highest density. An artificial electrical circuit-based model demonstrates dynamic increases synchronization over time, along predictions for selective adaptions connections. Environmental factors, like temperature absent microbiome, modify architecture, suggesting existence certain plasticity development. We propose that these features originated last common bilaterian ancestor, supporting hypothesis basic architecture universal.

Language: Английский

Citations

0