Wnt7b acts in concert with Wnt5a to regulate tissue elongation and planar cell polarity via noncanonical Wnt signaling DOI Creative Commons

Nicholas Xie,

André Landin Malt,

Aray Adylkhan

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(35)

Published: Aug. 22, 2024

Intercellular signaling mediated by evolutionarily conserved planar cell polarity (PCP) proteins aligns along the tissue plane and drives polarized behaviors during morphogenesis. Accumulating evidence indicates that vertebrate PCP pathway is regulated noncanonical, β-catenin-independent Wnt signaling; however, components mechanisms are incompletely understood. In mouse hearing organ, both noncanonical (ncWnt) required in developing auditory sensory epithelium to control cochlear duct elongation of resident hair cells (HCs), including shape orientation stereociliary bundle essential for sound detection. We have recently discovered a Wnt/G-protein/PI3K coordinates HC intercellular signaling. Here, we identify Wnt7b as ncWnt ligand acting concert with Wnt5a promote diverse developmental processes. cochlea, redundantly coiling elongation, polarity, asymmetric localization core Fzd6 Dvl2. Mechanistically, Wnt5a/Wnt7b-mediated promotes membrane recruitment Daple, nonreceptor guanine nucleotide exchange factor Gαi, activates PI3K/AKT ERK signaling, which localization. Thus, pathways distinct mutant phenotypes components, suggesting they act separate, parallel nonoverlapping functions NcWnt reinforces regulating trafficking PCP-specific Frizzled receptors.

Language: Английский

Wnt7b acts in concert with Wnt5a to regulate tissue elongation and planar cell polarity via noncanonical Wnt signaling DOI Creative Commons

Nicholas Xie,

André Landin Malt,

Aray Adylkhan

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(35)

Published: Aug. 22, 2024

Intercellular signaling mediated by evolutionarily conserved planar cell polarity (PCP) proteins aligns along the tissue plane and drives polarized behaviors during morphogenesis. Accumulating evidence indicates that vertebrate PCP pathway is regulated noncanonical, β-catenin-independent Wnt signaling; however, components mechanisms are incompletely understood. In mouse hearing organ, both noncanonical (ncWnt) required in developing auditory sensory epithelium to control cochlear duct elongation of resident hair cells (HCs), including shape orientation stereociliary bundle essential for sound detection. We have recently discovered a Wnt/G-protein/PI3K coordinates HC intercellular signaling. Here, we identify Wnt7b as ncWnt ligand acting concert with Wnt5a promote diverse developmental processes. cochlea, redundantly coiling elongation, polarity, asymmetric localization core Fzd6 Dvl2. Mechanistically, Wnt5a/Wnt7b-mediated promotes membrane recruitment Daple, nonreceptor guanine nucleotide exchange factor Gαi, activates PI3K/AKT ERK signaling, which localization. Thus, pathways distinct mutant phenotypes components, suggesting they act separate, parallel nonoverlapping functions NcWnt reinforces regulating trafficking PCP-specific Frizzled receptors.

Language: Английский

Citations

3