Homeostatic Control of Deep Sleep inDrosophila: Implications for Discovering Correlates of Sleep Pressure DOI Creative Commons
Budhaditya Chowdhury, Lakshman Abhilash, Antonio Ortega

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Oct. 2, 2022

Abstract Homeostatic control of sleep is typically addressed through mechanical stimulation-induced forced wakefulness and the measurement subsequent increases in sleep. A major confound attends this approach: biological responses to deprivation may reflect a direct response insult rather than loss Similar confounds accompany all forms represent challenge field. Here we describe new paradigm for Drosophila that fully accounts sleep-independent effects. Our results reveal deep states are primary target homeostatic establish presence multi-cycle rebound following deprivation. Furthermore, specific state state-specific rebound. Finally, by accounting molecular effects stimulation during experiments, show serotonin levels track pressure fly’s central brain. illustrate critical need when examining correlates call reassessment work has not accounted such non-specific

Language: Английский

Multivariate classification of multichannel long-term electrophysiology data identifies different sleep stages in fruit flies DOI Creative Commons
Sridhar R. Jagannathan,

Travis Jeans,

Matthew N. Van De Poll

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(8)

Published: Feb. 21, 2024

Identifying different sleep stages in humans and other mammals has traditionally relied on electroencephalograms. Such an approach is not feasible certain animals such as invertebrates, although these could also be sleeping stages. Here, we perform long-term multichannel local field potential recordings the brains of behaving flies undergoing spontaneous bouts. We acquired consistent spatial potentials across multiple flies, allowing us to compare brain activity awake periods. Using machine learning, uncover distinct temporal explore associated spectral features fly brain. Further, analyze electrophysiological correlates microbehaviors with confirm existence a stage rhythmic proboscis extensions show that this sleep-related behavior differ significantly from those same during wakefulness, indicating dissociation between states wherein behaviors reside.

Language: Английский

Citations

14
Experimentally induced active and quiet sleep engage non-overlapping transcriptional programs in Drosophila DOI Creative Commons
Niki Anthoney,

Lucy A.L. Tainton-Heap,

Ngoc Bao Hang Luong

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: June 14, 2023

Sleep in mammals can be broadly classified into two different physiological categories: rapid eye movement (REM) sleep and slow-wave (SWS), accordingly REM SWS are thought to achieve a set of functions. The fruit fly Drosophila melanogaster is increasingly being used as model understand functions, although it remains unclear if the brain also engages kinds well. Here, we compare commonly approaches for studying experimentally : optogenetic activation sleep-promoting neurons provision drug, gaboxadol. We find that these sleep-induction methods have similar effects on increasing duration, but divergent activity. Transcriptomic analysis reveals drug-induced deep (‘quiet’ sleep) mostly downregulates metabolism genes, whereas ‘active’ upregulates wide range genes relevant normal waking This suggests optogenetics pharmacological induction promote features sleep, which engage sets their respective

Language: Английский

Citations

11
Do insects have an inner life? Animal consciousness needs a rethink DOI

Mariana Lenharo

Nature, Journal Year: 2024, Volume and Issue: 629(8010), P. 14 - 15

Published: April 19, 2024

Language: Английский

Citations

2
Stem cell-specific ecdysone signaling regulates the development of dorsal fan-shaped body neurons and sleep homeostasis DOI Creative Commons
Adil R. Wani, Budhaditya Chowdhury,

Jenny Luong

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(21), P. 4951 - 4967.e5

Published: Oct. 8, 2024

Highlights•Sleep-regulating 23E10 dFB neurons originate from late larval type II NSCs•DL1 and DM1 NSCs specifically produce neurons•Ecdysone signaling in is essential for neuronal fate•E93 regulates fate sleep homeostasisSummaryComplex behaviors arise neural circuits that assemble diverse cell types. Sleep a conserved behavior survival, yet little known about how the nervous system generates neuron types of sleep-wake circuit. Here, we focus on specification Drosophila 23E10-labeled dorsal fan-shaped body (dFB) long-field tangential input project to layers neuropil central complex. We use lineage analysis genetic birth dating identify two bilateral stem cells (NSCs) generate neurons. show adult express ecdysone-induced protein 93 (E93) loss ecdysone or E93 results their misspecification. Finally, knockdown impairs behavior. Our provide insight into extrinsic hormonal acts diversity required These findings suggest some disorders might derive defects cell-specific temporal neurodevelopmental programs.Graphical abstract

Language: Английский

Citations

2
Homeostatic Control of Deep Sleep and Molecular Correlates of Sleep Pressure in Drosophila DOI Creative Commons
Budhaditya Chowdhury, Lakshman Abhilash, Antonio Ortega

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Oct. 31, 2023

Homeostatic control of sleep is typically addressed through mechanical stimulation-induced forced wakefulness and the measurement subsequent increases in sleep. A major confound attends this approach: biological responses to deprivation may reflect a direct response insult rather than loss Similar confounds accompany all forms represent challenge field. Here, we describe new paradigm for Drosophila that fully accounts sleep-independent effects. Our results reveal deep states are primary target homeostatic establish presence multi-cycle rebound following deprivation. Furthermore, specific state-specific rebound. Finally, by accounting molecular effects stimulation during experiments, show serotonin levels track pressure fly's central brain. illustrate critical need when examining correlates call reassessment work has not accounted such non-specific

Language: Английский

Citations

6
Multivariate classification of multichannel long-term electrophysiology data identifies different sleep stages in fruit flies DOI Creative Commons
Sridhar R. Jagannathan, Rhiannon Jeans, Matthew N. Van De Poll

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 13, 2023

ABSTRACT Sleep is observed in most animals, which suggests it subserves a fundamental process associated with adaptive biological functions. However, the evidence to directly associate sleep specific function lacking, part because not single many animals. In humans and other mammals, different stages have traditionally been identified using electroencephalograms (EEGs), but such an approach feasible animals as insects. Here, we perform long-term multichannel local field potential (LFP) recordings brains of behaving flies undergoing spontaneous bouts. We developed protocols allow for consistent spatial LFPs across multiple flies, allowing us compare LFP activity awake periods further same induced sleep. Using machine learning, uncover existence distinct temporal explore spectral features fly brain. Further, analyze electrophysiological correlates micro-behaviours certain stages. confirm stage rhythmic proboscis extensions show that this sleep-related behavior differ significantly from those during wakefulness, indicating dissociation between brain states wherein these behaviors reside.

Language: Английский

Citations

4
Intermittent Vibration Induces Sleep via an Allatostatin A‐GABA Signaling Pathway and Provides Broad Benefits in Alzheimer's Disease Models DOI Creative Commons
M. Yang, Yan Zhang,

Yuxian Zheng

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

While animals across species typically experience suppressed consciousness and an increased arousal threshold during sleep, the responsiveness to specific sensory inputs persists. Previous studies have demonstrated that rhythmic continuous vibration can enhance sleep in both humans. However, neural circuits underlying vibration-induced (VIS) its potential therapeutic benefits on neuropathological processes disease models remain unclear. Here, it is shown intermittent vibration, such as cycles of 30 s followed by off, more effective inducing compared vibration. A clear evidence further provided allatostatin (AstA)-GABA signaling mediates short-term (iVIS) inhibiting octopaminergic neurons through activating GABA

Language: Английский

Citations

1
To test the boundaries of consciousness, study animals DOI
Simon Alexander Burns Brown, Elizabeth S. Paul, Jonathan Birch

et al.

Trends in Cognitive Sciences, Journal Year: 2024, Volume and Issue: 28(10), P. 874 - 875

Published: Aug. 23, 2024

Language: Английский

Citations

1
Stem cell-specific ecdysone signaling regulates the development and function of aDrosophilasleep homeostat DOI Creative Commons
Adil R. Wani, Budhaditya Chowdhury,

Jenny Luong

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 2, 2023

Abstract Complex behaviors arise from neural circuits that are assembled diverse cell types. Sleep is a conserved and essential behavior, yet little known regarding how the nervous system generates neuron types of sleep-wake circuit. Here, we focus on specification Drosophila sleep-promoting neurons—long-field tangential input neurons project to dorsal layers fan-shaped body neuropil in central complex (CX). We use lineage analysis genetic birth dating identify two bilateral Type II stem cells generate these (dFB) neurons. show adult dFB express Ecdysone-induced protein E93, loss Ecdysone signaling or E93 NSCs results misspecification Finally, knockdown affects sleep behavior. Our provide insight into extrinsic hormonal acts neuronal diversity required for These findings suggest some disorders might derive defects cell-specific temporal neurodevelopmental programs.

Language: Английский

Citations

1
Whole-Brain Electrophysiology and Calcium Imaging inDrosophiladuring Sleep and Wake DOI
Matthew N. Van De Poll,

Lucy A.L. Tainton-Heap,

Michael Troup

et al.

Cold Spring Harbor Protocols, Journal Year: 2023, Volume and Issue: 2024(9), P. pdb.top108394 - pdb.top108394

Published: Dec. 26, 2023

Sleep is likely a whole-brain phenomenon, with most of the brain probably benefiting from this state decreased arousal. Recent advances in our understanding some potential sleep functions, such as metabolite clearance and synaptic homeostasis, make it evident why whole impacted by sleep: All neurons have synapses, all produce waste metabolites. experiments fly Drosophila melanogaster suggest that diverse functions appear to be conserved across animals. Studies activity during humans typically involve multidimensional data sets, those acquired electroencephalograms (EEGs) or functional magnetic resonance imaging (fMRI), these read-outs often reveal important qualities different stages, changes frequency dynamics connectivity. Recently, various techniques been developed allow for recording neural simultaneously multiple regions brain. These whole-brain-recording approaches will better physiology function, they provide more comprehensive view wake relevant model system. Here, we present brief summary findings derived studies sleeping flies discuss value electrophysiological versus calcium techniques. Although very preparations, both highlight studying system, like use EEG fMRI humans.

Language: Английский

Citations

1