The molecular diversity of hippocampal regions and strata at synaptic resolution revealed by integrated transcriptomic and proteomic profiling DOI Creative Commons
Eva Kaulich,

Quinn Waselenchuk,

N Furst

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 5, 2024

ABSTRACT The molecular diversity of neurons and their synapses underlies the different responses plasticity profiles that drive all neural circuits behavior. While extent this has been partially revealed by transcriptomic proteomic profiling, combined studies neuronal transcripts proteins are limited. Here, we used microdissection mouse hippocampal subregions CA1 strata fluorescence-activated synaptosome sorting (FASS) to characterize from compartments with synaptic resolution. Parallel RNA-seq LC-MS/MS microdissections identified over 15,000 mRNA 10,000 proteins, revealing thousands local enrichment such as classes glutamate receptors voltage-gated potassium channels, myelin-associated molecules, adhesion molecules. Synaptosome analysis further specific molecules collagen, ribosome, solute carrier, receptor families at formed along neurons. By integrating protein data, defined clusters co-regulated neurofilament transporter mRNAs, found subsets mRNA-protein pairs strong correlation anti-correlation in abundance variation. Our findings comprise a rich resource on landscape hippocampus its is accessible syndive.org , highlight coordinated organization between regions, compartments, synapses.

Language: Английский

The molecular diversity of hippocampal regions and strata at synaptic resolution revealed by integrated transcriptomic and proteomic profiling DOI Creative Commons
Eva Kaulich,

Quinn Waselenchuk,

N Furst

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 5, 2024

ABSTRACT The molecular diversity of neurons and their synapses underlies the different responses plasticity profiles that drive all neural circuits behavior. While extent this has been partially revealed by transcriptomic proteomic profiling, combined studies neuronal transcripts proteins are limited. Here, we used microdissection mouse hippocampal subregions CA1 strata fluorescence-activated synaptosome sorting (FASS) to characterize from compartments with synaptic resolution. Parallel RNA-seq LC-MS/MS microdissections identified over 15,000 mRNA 10,000 proteins, revealing thousands local enrichment such as classes glutamate receptors voltage-gated potassium channels, myelin-associated molecules, adhesion molecules. Synaptosome analysis further specific molecules collagen, ribosome, solute carrier, receptor families at formed along neurons. By integrating protein data, defined clusters co-regulated neurofilament transporter mRNAs, found subsets mRNA-protein pairs strong correlation anti-correlation in abundance variation. Our findings comprise a rich resource on landscape hippocampus its is accessible syndive.org , highlight coordinated organization between regions, compartments, synapses.

Language: Английский

Citations

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