A microfluidic twin islets-on-chip device for on-line electrophysiological monitoring

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Pancreatic islets play a major role in glucose homeostasis as well diabetes, and islets-on-chip devices have been mainly developed using optical means for on-line monitoring. In contrast, no well-characterized electrophysiological platform analysis with unrivalled temporal resolution has reported. Extracellular electrophysiology monitors two crucial parameters, islet β-cell activity β-to-β-cell coupling, does not require chemical or genetic probes inherent potential bias, is non-invasive permits repetitive long-term We now characterized microfluidic combined (on-line) hormone monitoring (off-line) chambers concomitant Fabrication of the device, based on commercial easily manufacturable components, within reach non-specialized laboratories. The chip convenient loading culture comparable kinetics low shear stress both chambers. An optimized flow rate did alter electrical coupling response to glucose. Culturing up 8 days change survival glucose-induced secretory β-cells. addition physiological amino acid mix, presence elevated glucose, made considerable functional organisation terms frequency which explains ensuing strong increase insulin secretion. This device thus allows reliable multiparametric populations. ease fabrication, assembly handling should permit widespread native micro-organs (e.g. controls/mutants), pseudo-islets stem-cell-derived islet-like organoids.

Language: Английский

Untangling the genetics of beta cell dysfunction and death in type 1 diabetes

Molecular Metabolism, Journal Year: 2024, Volume and Issue: 86, P. 101973 - 101973

Published: June 22, 2024

Type 1 diabetes (T1D) is a complex multi-system disease which arises from both environmental and genetic factors, resulting in the destruction of insulin-producing pancreatic beta cells. Over past two decades, human studies have provided new insight into etiology T1D, including an appreciation for role cells their own demise. Here, we outline models supported by data cell dysfunction death T1D. We highlight importance strong evidence linking T1D associations to bona fide candidate genes mechanistic therapeutic consideration. To guide rigorous interpretation associations, describe molecular profiling approaches, genomic resources, that may be used construct variant-to-gene links investigate profile advances understanding causes at individual risk loci. introduce prediction discuss how they can address heterogeneity. Finally, present areas where investment will critical future use genetics open questions develop treatment prevention strategies

Language: Английский

Garlic Extract Promotes Pancreatic Islet Neogenesis Through α‐to‐β‐Cell Transdifferentiation and Normalizes Glucose Homeostasis in Diabetic Rats

Molecular Nutrition & Food Research, Journal Year: 2024, Volume and Issue: 68(19)

Published: Aug. 29, 2024

Garlic extract (GE) has been shown to ameliorate hyperglycemia in diabetic rats (DRs) by increasing insulin production. However, the mechanism through which it exerts its effects remains unclear. Here, investigates molecular process and origin of regenerating β-cell with streptozotocin (STZ)-induced diabetes response GE.

Language: Английский