A synthetic method to assay polycystin channel biophysics DOI Creative Commons
Megan Larmore, Orhi Esarte Palomero, Neha P. Kamat

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: July 10, 2024

Ion channels are biological transistors that control ionic flux across cell membranes to regulate electrical transmission and signal transduction. They found in all their conductive state kinetics frequently disrupted human diseases. Organelle ion among the most resistant functional pharmacological interrogation. Traditional channel protein reconstitution methods rely upon exogenous expression and/or purification from endogenous cellular sources which contaminated by resident ionophores. Here, we describe a fully synthetic method assay properties of polycystin natively traffic primary cilia endoplasmic reticulum organelles. Using this method, characterize oligomeric assembly, membrane integration, orientation, conductance while comparing these results properties. Outcomes define novel approach can be applied broadly investigate biophysical analysis characterization.

Language: Английский

Lysosomal Ion Channels and Transporters: Recent Findings, Therapeutic Potential, and Technical Approaches DOI
Artem Kondratskyi, Andre Bazzone, Markus Rapedius

et al.

Bioelectricity, Journal Year: 2025, Volume and Issue: 7(1), P. 29 - 57

Published: March 1, 2025

Language: Английский

Citations

0
A synthetic method to assay polycystin channel biophysics DOI Creative Commons
Megan Larmore, Orhi Esarte Palomero, Neha P. Kamat

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: July 10, 2024

Ion channels are biological transistors that control ionic flux across cell membranes to regulate electrical transmission and signal transduction. They found in all their conductive state kinetics frequently disrupted human diseases. Organelle ion among the most resistant functional pharmacological interrogation. Traditional channel protein reconstitution methods rely upon exogenous expression and/or purification from endogenous cellular sources which contaminated by resident ionophores. Here, we describe a fully synthetic method assay properties of polycystin natively traffic primary cilia endoplasmic reticulum organelles. Using this method, characterize oligomeric assembly, membrane integration, orientation, conductance while comparing these results properties. Outcomes define novel approach can be applied broadly investigate biophysical analysis characterization.

Language: Английский

Citations

2