The structure of basal body inner junctions from Tetrahymena revealed by electron cryo-tomography DOI Creative Commons
Sam Li, José‐Jesús Fernández, Marisa D. Ruehle

et al.

The EMBO Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

Abstract The cilium is a microtubule-based eukaryotic organelle critical for many cellular functions. Its assembly initiates at basal body and continues as an axoneme that projects out of the cell to form functional cilium. This process tightly regulated. However, our knowledge molecular architecture mechanism limited. By applying cryo-electron tomography, we obtained structures inner junction in three regions from Tetrahymena : proximal, central core body, axoneme. We identified several protein components body. While few proteins are distributed throughout entire length organelle, restricted specific regions, forming intricate local interaction networks bolstering structural stability. examining POC1 knockout mutant, found triplet microtubule was destabilized, resulting defective structure. Surprisingly, axoneme-specific were “infiltrate” into mutant Our findings provide insight junctions, underscoring its precise spatial regulation.

Language: Английский

The structure of basal body inner junctions from Tetrahymena revealed by electron cryo-tomography DOI Creative Commons
Sam Li, José‐Jesús Fernández, Marisa D. Ruehle

et al.

The EMBO Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

Abstract The cilium is a microtubule-based eukaryotic organelle critical for many cellular functions. Its assembly initiates at basal body and continues as an axoneme that projects out of the cell to form functional cilium. This process tightly regulated. However, our knowledge molecular architecture mechanism limited. By applying cryo-electron tomography, we obtained structures inner junction in three regions from Tetrahymena : proximal, central core body, axoneme. We identified several protein components body. While few proteins are distributed throughout entire length organelle, restricted specific regions, forming intricate local interaction networks bolstering structural stability. examining POC1 knockout mutant, found triplet microtubule was destabilized, resulting defective structure. Surprisingly, axoneme-specific were “infiltrate” into mutant Our findings provide insight junctions, underscoring its precise spatial regulation.

Language: Английский

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