Synthase-selected sorting approach identifies a beta-lactone synthase in a nudibranch symbiotic bacterium

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: June 13, 2023

Abstract Background Nudibranchs comprise a group of > 6000 marine soft-bodied mollusk species known to use secondary metabolites (natural products) for chemical defense. The full diversity these and whether symbiotic microbes are responsible their synthesis remains unexplored. Another issue in searching undiscovered natural products is that computational analysis genomes uncultured can result detection novel biosynthetic gene clusters; however, vivo functionality not guaranteed which limits further exploration pharmaceutical or industrial potential. To overcome challenges, we used fluorescent pantetheine probe, produces CoA-analog employed biosynthesis metabolites, label capture bacterial symbionts actively producing compounds the mantle nudibranch Doriopsilla fulva. Results We recovered genome Candidatus Doriopsillibacter californiensis from Ca. Tethybacterales order, an lineage sponge found nudibranchs previously. It forms part core skin microbiome D. fulva nearly absent its internal organs. showed crude extracts contained were consistent with presence beta-lactone encoded genome. Beta-lactones represent underexplored potential have been reported Conclusions Altogether, this study shows how probe-based, targeted sorting approaches vivo.

Language: Английский

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Metagenomic and metatranscriptomic exploration of the Egyptian Red Sea sponge Theonella sp. associated microbial community

Marine Genomics, Journal Year: 2023, Volume and Issue: 70, P. 101032 - 101032

Published: April 19, 2023

Language: Английский

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Bioactivity of bacteria associated with Red Sea nudibranchs and whole genome sequence of Nocardiopsis dassonvillei RACA-4

Marine Genomics, Journal Year: 2022, Volume and Issue: 67, P. 101004 - 101004

Published: Dec. 13, 2022

Language: Английский

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Metabolomic Profiling and Molecular Networking of Nudibranch-Associated Streptomyces sp. SCSIO 001680

Molecules, Journal Year: 2022, Volume and Issue: 27(14), P. 4542 - 4542

Published: July 16, 2022

Antibiotic-resistant bacteria are the primary source of one growing public health problems that requires global attention, indicating an urgent need for new antibiotics. Marine ecosystems characterized by high biodiversity and considered essential sources bioactive chemical compounds. Bacterial associates marine invertebrates commonly a active medicinal natural products important drug discovery. Hence, invertebrate-associated microbiomes fruitful resource excavating novel genes In previous study, we isolated Streptomyces sp. SCSIO 001680, coded as strain 63, from Red Sea nudibranch Chromodoris quadricolor, which exhibited antimicrobial antitumor activity. addition, this isolate harbors several product biosynthetic gene clusters, suggesting it has potential to produce products. The present study aimed investigate metabolic profile 001680 (strain 63) predict their role in host's survival. crude extracts 63 cultivated two different media were ultra-high-performance liquid chromatography high-resolution mass spectrometry. metabolomics approach provided us with characteristic fingerprints cellular processes relative abundance specific Global Products Social Molecular Networking database was used identify metabolites. While 434 metabolites detected extracts, only few compounds identified based on standards spectral libraries, including desferrioxamines, marineosin A, bisucaberin, halichoblelide, alternarin pachastrelloside streptodepsipeptide P1 1B, didemnaketal F, alexandrolide. This finding suggests metabolism microbiome remains poorly represented. Thus, our data constitute valuable complement host microbiome.

Language: Английский

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Mining Chromodoris quadricolor symbionts for biosynthesis of novel secondary metabolites

Marine Genomics, Journal Year: 2023, Volume and Issue: 68, P. 101017 - 101017

Published: Feb. 2, 2023

Language: Английский

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Microbiome origin and stress-related changes in bacterial abundance of the photosymbiotic sea slug Berghia stephanieae (Á. Valdés, 2005)

Symbiosis, Journal Year: 2024, Volume and Issue: 93(2), P. 177 - 192

Published: June 1, 2024

Abstract The precise mechanisms that allow animals and phototrophic organisms to form a stable photosymbiotic relationship are still unknown. While previous studies focused on genomic adaptations of the animal host, more recent research looked into role bacteria in photosymbiosis. Here, we analyzed core microbiome sea slug Berghia stephanieae its food source Exaiptasia diaphana understand if linked bacterial metabolic pathways differ between unstable This feeds solely model cnidarian E. steals their photobionts which can only maintain for week. We additionally examined influence light starvation stress slug’s composition, common experimental set-ups elucidate slugs. Our results show B. differed significantly suggesting is not obtained from or water column indicates vertical transmission. Further, differences imply does support photosymbiosis due an insufficient nitrogen cycle part photobiont. Starving slugs induced shift towards increased abundance potential pathogens led downregulation sulphur cycle. Yet, darkness resulted depletion most switch toward fixation. emphasizes different holobiont members contribute essential nutrient cycles, it important look beyond photobiont holobiont.

Language: Английский

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Enhancing hydrogen production from anaerobic digestion of pretreated fruit and vegetable peels using Clostridium butyricum NE133

Biomass and Bioenergy, Journal Year: 2024, Volume and Issue: 191, P. 107485 - 107485

Published: Nov. 12, 2024

Language: Английский

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Shotgun metagenomic analysis of bacterial symbionts associated with “Chromodoris quadricolor” mantle

Marine Genomics, Journal Year: 2023, Volume and Issue: 69, P. 101030 - 101030

Published: April 11, 2023

Language: Английский

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Exploring Prokaryotic Communities in the Guts and Mucus of Nudibranchs, and Their Similarity to Sediment and Seawater Microbiomes

Current Microbiology, Journal Year: 2023, Volume and Issue: 80(9)

Published: July 22, 2023

In the present study, we compared mucus and gut-associated prokaryotic communities from seven nudibranch species with sediment seawater Thai coral reefs using high-throughput 16S rRNA gene sequencing. The were identified as Doriprismatica atromarginata (family Chromodorididae), Jorunna funebris Discodorididae), Phyllidiella nigra, pustulosa, Phyllidia carlsonhoffi, elegans, picta (all family Phyllidiidae). most abundant bacterial phyla in dataset Proteobacteria, Tenericutes, Chloroflexi, Thaumarchaeota, Cyanobacteria. Mucus differed one another communities. Host phylogeny was, furthermore, a significant predictor of differences community composition. With respect to higher taxon abundance, order Rhizobiales (Proteobacteria) was more (mucus gut), whereas Mycoplasmatales (Tenericutes) D. J. funebris. samples were, associated greater abundances certain including Poribacteria, Gemmatimonadetes, taxa considered be indicators for high microbial abundance (HMA) sponge species. Overall, our results indicated that microbiomes consisted number members sequence similarities organisms previously detected sponges.

Language: Английский

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Bacterial and Fungal Co-Occurrence in the Nudibranch, Pteraeolidia semperi

Life, Journal Year: 2022, Volume and Issue: 12(12), P. 1988 - 1988

Published: Nov. 28, 2022

Despite the increasing recognition and importance surrounding bacterial fungal interactions, their critical contributions to ecosystem functioning host fitness, studies examining co-occurrence remain in infancy. Similarly, have yet characterise communities associated with nudibranchs or core microbial members. Doing this can advance our understanding of how microbiome helps a adapt persist its environment. In study, we characterised 46 Pteraeolidia semperi nudibranch individuals collected from four offshore islands Singapore. We found no distinct spatial structuring community, richness, diversity across sampling locations. The genera Mycoplasma Endozoicomonas were all samples islands. genus Leucoagaricus was highest occurrence, but not everywhere, is first record reported presence marine environments. network suggests that interactions are limited, identified family Colwelliaceae as potential keystone taxon disproportionately high number edges. Furthermore, clusters together other families such Pseudoalteromonadaceae Alteromonadaceae, which possible roles digestion food.

Language: Английский

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