Metabolites
present
in
the
hemolymph
of
honey
bees
play
a
crucial
role
modulating
metamorphic
process
within
species.
However,
precise
alterations
metabolite
composition,
along
with
associated
variances
and
regulatory
pathways
implicated
during
larvae-to-pupae
metamorphosis
bees,
remain
incompletely
elucidated.
In
this
investigation,
we
gathered
samples
from
bee
larvae
at
three
distinct
physiological
stages-feeding,
prepupal,
pupae
subjected
them
to
analysis
utilizing
liquid
chromatography-mass
spectrometry
(LC-MS)
technique.
Employing
principal
component
(PCA)
partial
least
squares-discriminant
(PLS-DA),
identified
significant
differential
metabolites
conducted
metabolic
pathway
on
those
differentially
up-regulated
prepupal
stages.
Notably,
stage
primarily
governed
glucose
metabolism
fat
digestion
absorption,
while
were
involved
regulating
chitin
lipopolysaccharide
precursor
formation,
as
well
biosynthesis
phenylalanine,
tyrosine,
tryptophan.
These
findings
bear
implications
for
advancing
our
comprehension
processes
bees.
Current Research in Insect Science,
Journal Year:
2023,
Volume and Issue:
3, P. 100055 - 100055
Published: Jan. 1, 2023
Fat
reserves,
specifically
the
accumulation
of
triacylglycerols,
are
a
major
energy
source
and
play
key
role
for
life
histories.
is
conserved
metabolic
pattern
across
most
insects,
yet
in
parasitoid
species
adults
do
not
gain
fat
mass,
even
when
nutrients
readily
available
provided
ad
libitum.
This
extraordinary
physiological
phenotype
has
evolved
repeatedly
phylogenetically
dispersed
species.
poses
conundrum
because
it
could
lead
to
significant
constraints
on
allocation
toward
adult
functions
such
as
survival
reproduction.
Recent
work
underlying
genetic
biochemical
mechanisms
spurred
debate
versus
production,
incongruent
interpretation
results
obtained
using
different
methodologies.
part
due
semantics,
highlighting
need
synthetic
perspective
that
reconciles
previous
debates
provides
new
insights
terminology.
In
this
paper,
we
propose
updated,
unambiguous
terminology
future
research
field,
including
"fatty
acid
synthesis"
"lack
accumulation",
describe
distinct
pathways
involved
complex
process
lipogenesis.
We
then
discuss
benefits
drawbacks
main
methods
measure
fatty
synthesis
accumulation.
Most
importantly,
gravimetric/colorimetric
isotope
tracking
give
complementary
information,
they
applied
with
appropriate
controls
interpreted
correctly.
also
compiled
comprehensive
list
studies
performed
during
last
25
years.
present
avenues
combine
chemistry,
ecology,
evolution
into
an
integrative
approach,
which
think
needed
understand
dynamics
parasitoids.
Insect Molecular Biology,
Journal Year:
2024,
Volume and Issue:
33(4), P. 350 - 361
Published: March 2, 2024
Abstract
Fatty
acyl‐CoA
reductase
(FAR)
is
one
of
the
key
enzymes,
which
catalyses
conversion
fatty
to
corresponding
alcohols.
Among
FAR
family
members
in
brown
planthopper
(
Nilaparvata
lugens
),
NlFAR7
plays
a
pivotal
role
both
synthesis
cuticular
hydrocarbons
and
waterproofing
cuticle.
However,
precise
mechanism
by
influences
formation
cuticle
structure
N.
remains
unclear.
Therefore,
this
paper
aims
investigate
impact
through
RNA
interference,
transmission
electron
microscope,
focused
ion
beam
scanning
microscopy
(FIB‐SEM)
lipidomics
analysis.
FIB‐SEM
employed
reconstruct
three‐dimensional
(3D)
architecture
pore
canals
related
structures
subjected
ds
GFP
treatments,
enabling
comprehensive
assessment
changes
structures.
The
results
reveal
reduction
thickness
disruptions
spiral
canals,
accompanied
widened
base
middle
diameters.
Furthermore,
comparison
analysis
between
‐
‐treated
demonstrated
that
there
were
25
metabolites
involved
lipid
layer
synthesis,
including
7
triacylglycerols
(TGs),
5
phosphatidylcholines
(PCs),
3
phosphatidylethanolamines
(PEs)
2
diacylglycerols
(DGs)
decreased,
4
(TGs)
PEs
increased.
In
conclusion,
silencing
disrupts
overall
lipids
destroys
structures,
thereby
disrupting
secretion
lipids,
thus
affecting
.
These
findings
give
significant
attention
with
reference
further
biochemical
researches
on
substrate
specificity
protein,
molecular
regulation
mechanisms
during
life
cycle.
Insects,
Journal Year:
2024,
Volume and Issue:
15(10), P. 771 - 771
Published: Oct. 6, 2024
Black
soldier
fly
larvae
(BSFL)
can
convert
organic
waste
into
high-quality
biomass.
In
this
study,
we
tested
the
potential
of
sugarcane
by-products
as
a
food
source
for
BSFL
and
compared
larval
development
nutritional
value
with
some
other
substrates.
Seven
different
substrates
were
used,
including
carrot
pomace
(C),
leftover
bread
(CB)
(50/50),
bagasse
vinasse
(BV),
molasses
(BM),
bagasse,
vinasse,
(BVM),
mixture
all
above
treatments
(MX),
university
canteen
leftovers
(UCLs).
The
weight
length
measured
two
weeks
from
day
5
to
19.
Then,
harvested
analyzed
dry
matter,
crude
protein,
oil,
ash,
mineral,
fatty
acid
composition.
Larval
varied
depending
on
feeding
substrate
provided.
University
resulted
in
having
at
least
18%
greater
(17.00
mm)
56%
(3.15
g)
treatments.
highest
amounts
protein
(38.9%)
oil
(39.06%)
observed
UCL
treatment,
while
BV
treatment
had
quantities
ash
(28.9%)
matter
(28.0%).
profile
revealed
three-times-higher
levels
saturated
acids
than
unsaturated
was
twice
high
Overall,
best
growth
substrate,
combination
(BV)
most
appropriate
among
by-products.
