Transforming marine monitoring: Using eDNA metabarcoding to improve the monitoring of the Mediterranean Marine Protected Areas network

Marine Policy, Journal Year: 2023, Volume and Issue: 156, P. 105807 - 105807

Published: Aug. 25, 2023

The creation of Marine Protected Areas (MPAs) is central to the European Union's strategy for protecting and restoring marine environments. However, current research indicates that monitoring MPAs insufficient ensure their protective role. In response, Commission encourages development adoption more effective tools MPAs. Environmental DNA (eDNA) methods may serve this function, but use in MPA management remains relatively unexplored. study addresses gap by examining potential eDNA enhance within Mediterranean MPAs, where challenges are especially severe. We addressed synthesizing results two analyses. First, a systematic literature review examined existing applications metabarcoding monitor habitats. From experiences documented reviewed literature, we applied SWOT analysis (a framework used identify analyze strengths, weaknesses, opportunities threats) assess tool's appropriateness EU requirements. Second, probed suitability with questionnaire on other completed managers 29 region. findings our demonstrated when compared conventional methods, frequently outperformed them or acted as crucial supplement. also found suitable most criteria set forth Our revealed limitations approaches alleviate. Still, do have drawbacks which jeopardize its accuracy sufficiency. Incorporating has great protection efforts elsewhere. Their successful will require improved coordination among stakeholders current, comprehensive, customized recommendations managers. This improve overall functioning networks thus support role safeguarding vulnerable

Language: Английский

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Three steps towards comparability and standardization among molecular methods for characterizing insect communities

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1904)

Published: May 5, 2024

Molecular methods are currently some of the best-suited technologies for implementation in insect monitoring. However, field is developing rapidly and lacks agreement on methodology or community standards. To apply DNA-based large-scale monitoring, to gain insight across commensurate data, we need easy-to-implement standards that improve data comparability. Here, provide three recommendations how harmonize efforts biodiversity assessment monitoring via metabarcoding: (i) should adopt use synthetic spike-ins, which will act as positive controls internal standards; (ii) consider using several markers through a multiplex polymerase chain reaction (PCR) approach; (iii) commit publication transparency all protocol-associated metadata standardized fashion. For (i), ready-to-use recipe cytochrome

Language: Английский

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Centering accessibility, increasing capacity, and fostering innovation in the development of international eDNA standards

Metabarcoding and Metagenomics, Journal Year: 2024, Volume and Issue: 8

Published: Sept. 24, 2024

Environmental DNA (eDNA) includes a set of rapidly emerging technologies that have the potential to support environmental monitoring and biodiversity conservation through novel, non-invasive, cost-effective democratic methods tools. Meanwhile, eDNA researchers are developing international standards for technologies, data outputs. For be accessible, useful appropriate, we must ensure any developed include broad conception users from around world, diversity ecological contexts locations and, most importantly, realistic outlook on research capacities infrastructure. In this article, assemble perspectives standardisation diverse global group experts Africa, South America Pacific Islands. The authors article collaborated by answering discussing open-ended questions aimed at eliciting hopes, concerns experiences regarding standards. result is emergent themes generative consensus highlight need creation adaptable standards, development regional capacity, increased sensitising sovereignty viewing as capacity-building activity.

Language: Английский

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Environmental DNA and its role in identifying yeast biodiversity in mangrove ecosystem: A literature review

E3S Web of Conferences, Journal Year: 2025, Volume and Issue: 605, P. 03005 - 03005

Published: Jan. 1, 2025

Yeast biodiversity in mangrove ecosystems is crucial for their health and ecological functions. However, traditional methods often fail to detect yeast species that are difficult culture or slow growing, leaving a significant portion of this undetected. This study explores the use environmental DNA (eDNA) as an alternative method identifying ecosystems, addressing data gaps left by conventional methods. Data collected from various relevant literature sources, including scientific journals, books, research reports. The review reveals eDNA analysis can improve conservation management providing robust assessments. Future should focus on understanding persistence, implications, improving bioinformatics better interpretation. In-depth long-term studies recommended temporal spatial dynamics communities ecosystems. Further validation conditions needed ensure reliability. Research interactions with other ecosystem components impact human activities like pollution habitat change also important.

Language: Английский

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eDNA-based approaches advance ecotoxicology: Insights and best practices from eDNA metabarcoding studies in evaluating stress-induced aquatic (macro-) invertebrate community composition

Ecological Indicators, Journal Year: 2025, Volume and Issue: 172, P. 113269 - 113269

Published: Feb. 28, 2025

Language: Английский

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Environmental DNA Template Variation: Its Relevance for Species Detection and Conservation

Freshwater Biology, Journal Year: 2025, Volume and Issue: 70(3)

Published: March 1, 2025

ABSTRACT Accurate species monitoring is foundational for understanding and assessing extinction risk. Environmental DNA (eDNA) based detection methods have been proposed as fast powerful biodiversity tools. Yet, these are susceptible to errors that might hinder the assessment of Samples may contain low concentrations target taxa and/or exhibit high levels PCR inhibitors, which can yield false negatives. We investigated how adjusting input sample volume in eDNA‐based molecular assay improves an endangered fish, Anaecypris hispanica , highly eutrophic streams. Water samples were filtered tested using a real‐time (qPCR) varying eDNA (i.e., 0.5X, 1X 3.3X). From positive detections obtained with different volumes, we built occurrence maps estimated geographic range metrics used risk assessment. Although number sites was similar among volumes tested, not spatially redundant. When comparing pooled results from all trials fixed volume, there nearly 75% increase detections, consequently leading increases extent occurrence, area occupancy locations). Our highlight negatives surveys be overlooked. The success will likely vary on case‐by‐case basis, depending concentration potential inhibitors bulk samples, both generally unknown. Improved achieved by running, parallel, qPCR assays samples. As interest grows integrating tools into practices, it essential continuously refine protocols carefully consider study design decisions ensure robust results, advancing management conservation.

Language: Английский

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Advancing the environmental DNA and RNA toolkit for aquatic ecosystem monitoring and management

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19119 - e19119

Published: March 18, 2025

The application of environmental DNA (eDNA) and RNA (eRNA) technologies to aquatic ecosystem monitoring management has increased rapidly in the last decade. These methods are providing many new exciting opportunities for enhanced biodiversity assessment, ecological health evaluation, species detection. This special issue PeerJ Life Environment brings together 20 innovative studies that collectively advance eDNA toolkit. Four key themes covered: (i) Methodological advancements, (ii) Ecological assessments biomonitoring, (iii) Species detection, (iv) Application management. cover a suite topics including; optimizing sample collection, developing species-specific assays, evaluating bioindicator species, assessing microbial activity, diverse freshwater marine habitats. Emerging applications, such as use genome skimming identify fish markers, showcase advancements this field. also highlight challenges, including need standardized protocols ethical considerations must be addressed before these tools can implemented or adopted decision making at national global scales. Together, contributions demonstrate transformative potential nucleic acids' advancing conservation By bridging methodological rigor with applied research, provide an important resource researchers, policymakers, practitioners committed sustainable stewardship.

Language: Английский

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Utilizing aquatic environmental DNA to address global biodiversity targets

Published: April 27, 2025

Language: Английский

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The MIEM guidelines: Minimum information for reporting of environmental metabarcoding data

Metabarcoding and Metagenomics, Journal Year: 2024, Volume and Issue: 8

Published: Dec. 30, 2024

Environmental DNA (eDNA) and RNA (eRNA) metabarcoding has become a popular tool for assessing biodiversity from environmental samples, but inconsistent documentation of methods, data metadata makes results difficult to reproduce synthesise. A working group scientists have collaborated produce set minimum reporting guidelines the constituent steps workflows, physical layout laboratories through archiving. We emphasise how suite should adhere findable, accessible, interoperable reproducible (FAIR) standards, thereby providing context evaluating understanding study results. An overview considerations each workflow step is presented then summarised in checklist that can accompany published or report. Ensuring workflows are transparent documented critical research allow more efficient uptake into management decision-making.

Language: Английский

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Welcoming More Participation in Open Data Science for the Oceans

Annual Review of Marine Science, Journal Year: 2023, Volume and Issue: 16(1), P. 537 - 549

Published: July 7, 2023

Open science is a global movement happening across all research fields. Enabled by technology and the open web, it builds on years of efforts individuals, grassroots organizations, institutions, agencies. The goal to share knowledge broaden participation in science, from early ideation making outputs openly accessible (open access). With an emphasis transparency collaboration, dovetails with increase diversity, equity, inclusion, belonging society. US Biden-Harris Administration many other government agencies have declared 2023 Year Science, providing great opportunity boost for oceans. For researchers day-to-day, critical piece modern analytical workflows increasing amounts data. Therefore, we focus this article data science-the tooling people enabling reproducible, transparent, inclusive practices data-intensive research-and its intersection marine sciences. We discuss state various dimensions argue that technical advancements outpaced our field's culture change incorporate them. Increasing inclusivity skill building are interlinked must be prioritized within community find collaborative solutions responding climate threats biodiversity

Language: Английский

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