Cited by Molecular Species Determination of Cyathostomins from Horses in Ireland

A national survey of anthelmintic resistance in ascarid and strongylid nematodes in Australian Thoroughbred horses DOI

Ghazanfar Abbas, Abdul Ghafar, Emma McConnell

et al.

International Journal for Parasitology Drugs and Drug Resistance, Journal Year: 2023, Volume and Issue: 24, P. 100517 - 100517

Published: Nov. 29, 2023

This study quantified the extent of anthelmintic resistance (AR) to ascarid and strongylid nematodes among commonly used anthelmintics in Australian Thoroughbred horses. Faecal egg count reduction tests (FECRTs, n = 86) reappearance period (ERP) were conducted on 22 farms across Australia. counts (FECs) determined using modified McMaster technique, percent faecal (%FECR) was calculated Bayesian hierarchical model hybrid Frequentist/Bayesian analysis method. The results interpreted old (published 1992) new (2023) research guidelines World Association for Advancement Veterinary Parasitology (WAAVP). species composition detected utilising a DNA-metabarcoding method pre- post-treatment samples. Resistance observed single-active combination anthelmintics, including ivermectin (IVM %FECR range: 82%–92%; 95% lower credible interval (LCI) 80%–90%), abamectin (ABM: 73%–92%; 65%–88%), moxidectin (MOX: 89%–91%; 84%–89%), oxfendazole (OFZ: 0%–56%; 0%–31%) its with pyrantel (OFZ + PYR: 0%–82%; 0%–78%). Parascaris spp. IVM (10%–43%; 0%–36%), ABM (0%; 0%) MOX 0%). When thresholds recommended by WAAVP used, AR six additional FECRTs strongylids three more spp., introducing OFZ PYR latter. Shortened ERPs (4–6 weeks) cyathostomins 31 which not at 2 weeks all tested. Among cyathostomins, Cylicocyclus nassatus, Cylicostephanus longibursatus Coronocyclus coronatus most prevalent post-treatment, whereas main appearing five following treatments macrocyclic lactones ashworthi. After treatment PYR, latter three, plus Cyathostomum catinatum, 5 post-treatment. Overall, highlights prevalence both ascarids against products control worms indicate that ML provided acceptable efficacy weeks. However, ERP calculations suggest work less effectively than previously measured. It is suggested regularly monitor consider changing worm practices better manage

Language: Английский

Citations

Molecular species determination of cyathostomins from horses in Ireland DOI

Orla Byrne,

Disha Gangotia,

John Crowley

et al.

Veterinary Parasitology, Journal Year: 2024, Volume and Issue: 328, P. 110168 - 110168

Published: March 15, 2024

Cyathostomins are globally important equine parasites, responsible for both chronic and acute pathogenic effects. The occurrence of mixed infections with numerous cyathostomin species hinders our understanding parasite epidemiology, host-parasite dynamics, pathogenicity. There have been few studies occurring in horses Ireland, where temperate climatic conditions year-round rainfall provide suitable infection grazing animals bursate nematodes. Here, we amplified sequenced the ITS-2 region adult worms harvested at post-mortem from 11 between August 2018 June 2020, recorded prevalence abundance recovered caecum, right ventral colon left dorsal colon, using BLAST IDTAXA taxonomic attribution. Phylogenetic relationships community composition were also compared other relevant studies, including a global meta-analysis. Overall, results agree previous that there does not seem to be major difference equids different geographical regions. We confirmed workers relation difficulties discriminating Cylicostephanus calicatus Coronocyclus coronatus on basis sequences.

Language: Английский

Citations

Comparative phylogenetic and sequence identity analysis of internal transcribed spacer 2 and cytochrome C oxidase subunit I as DNA barcode markers for the most common equine Strongylidae species DOI

Irina Diekmann, Jürgen Krücken, Тetiana А. Kuzmina

et al.

Infection Genetics and Evolution, Journal Year: 2025, Volume and Issue: unknown, P. 105729 - 105729

Published: Feb. 1, 2025

Language: Английский

Citations

Understanding temporal and spatial distribution of intestinal nematodes of horses using faecal egg counts and DNA metabarcoding DOI

Ghazanfar Abbas, Abdul Ghafar, Anne Beasley

et al.

Veterinary Parasitology, Journal Year: 2023, Volume and Issue: 325, P. 110094 - 110094

Published: Dec. 3, 2023

This study reports the spatial and temporal distribution of ascarid strongylid nematodes in Thoroughbred horses by age category across different climatic zones Australia over an 18-month period. Faecal samples (n = 2046) from individual were analysed using modified McMaster technique for faecal egg counts (FECs). Strongylids identified PCR-directed next-generation sequencing second internal transcribed spacer (ITS-2) nuclear ribosomal DNA. Yearlings had highest prevalence (82%) strongyle eggs followed weanlings (79%), foals (58%), wet mares (49%) dry (46%). For Parascaris spp., (35%) (21%) yearlings (10%). The mean FECs spp. observed (525 per gram [EPG] faeces) while those strongyles (962 EPG). Among that classified as adults at time sampling, 77% (860 1119) low (i.e., <250 EPG) egg-shedders. Mean FEC Mediterranean (818 summer (599 EPG), winter (442 non-seasonal (413 rainfall zones. Twenty-six nematode species detected, with Cylicostephanus longibursatus (26.5%), Cylicocyclus nassatus (23.7%) Coronocyclus coronatus (20.5%) being most frequently detected species. Their richness relative abundance varied horse age, season zone. In addition, Strongylus equinus Triodontophorus (T. brevicauda T. serratus) also detected. comprehensive elucidates (climatic zone) seasonal) trends burdens intestinal Australian non-invasive conventional molecular methods. information presented this is crucial developing integrated management strategies to control parasites farmed horses.

Language: Английский

Citations

Mixed strongyle parasite infections vary across host age and space in a population of feral horses DOI

Sangwook Ahn,

Elizabeth Redman,

Stefan Gavriliuc

et al.

Parasitology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 18

Published: Dec. 12, 2024

Abstract Identifying factors that drive among-individual variation in mixed parasitic infections is fundamental to understanding the ecology and evolution of host–parasite interactions. However, a lack non-invasive diagnostic tools quantify has restricted their investigation for host populations wild. This study applied DNA metabarcoding on parasite larvae cultured from faecal samples characterize strongyle 320 feral horses Sable Island, Nova Scotia, Canada, 2014 test influence (age, sex reproductive/social status) environmental (location, local density social group membership) variation. Twenty-five species were identified, with individual ranging 3 18 mean richness (±1 s.d. ) 10.8 ± 3.1. Strongyle eggs shed faeces dominated by small (cyathostomins) young individuals, transitioning large strongyles ( Strongylus spp.) adults. Egg counts highest individuals west or centre island most species. Individuals same had similar communities, supporting hypothesis shared environment may assemblages. Other such as horse density, sex, date status minimal impacts infection patterns. demonstrates can be dynamic across ontogeny space emphasizes need consider species-specific patterns when investigating infections.

Language: Английский

Citations

Chicory (Cichorium intybus) reduces cyathostomin egg excretion and larval development in grazing horses DOI

Joshua Malsa, Leslie Boudesocque‐Delaye,

Laurence Wimel

et al.

International Journal for Parasitology Drugs and Drug Resistance, Journal Year: 2024, Volume and Issue: 24, P. 100523 - 100523

Published: Feb. 9, 2024

Cyathostomins are the most prevalent parasitic nematodes of grazing horses. They responsible for colic and diarrhea in their hosts. After several decades exposure to synthetic anthelmintics, they have evolved become resistant compounds. In addition, drug-associated environmental side-effects question use field. Alternative control strategies, like bioactive forages, needed face these challenges. Among these, chicory (Cichorium intybus, Puna II cultivar (cv.)) is known convey anthelmintic compounds may cyathostomins To challenge this hypothesis, we measured fecal egg counts rate larval development 20 naturally infected young saddle horses (2-year-old) either (i) a pasture sown with (n = 10) or (ii) mesophile grassland at same stocking (2.4 livestock unit (LU)/ha). The period lasted 45 days prevent horse reinfection. Horses group mostly grazed (89% bites), while those mainly grasses (73%). excretion decreased both groups throughout experiment. Accounting trajectory, count reduction (FECR) individuals relative increased from 72.9% day 16–85.5% end study. feces on was reduced by more than 60% d31 compared individuals. Using metabarcoding approach, also evidenced significant decrease cyathostomin species abundance chicory. Chicory extract enriched sesquiterpenes lactones tested two isolates. estimated IC50 high (1 3.4 mg/ml) varied according pyrantel sensitivity status worm isolate. We conclude that (cv. II) promising strategy reducing contribute lower reliance anthelmintics. underpinning modes action remain be explored further.

Language: Английский

Citations

Gastrointestinal nematodes in German outdoor-reared pigs based on faecal egg count and next-generation sequencing nemabiome data DOI

Hannah R M Fischer, Jürgen Krücken, S. Fiedler

et al.

Porcine Health Management, Journal Year: 2024, Volume and Issue: 10(1)

Published: Sept. 12, 2024

Language: Английский

Citations

Spatio-temporal diversity and genetic architecture of pyrantel resistance inCylicocyclus nassatus, the most abundant horse parasite DOI

Guillaume Sallé, Élise Courtot, Cédric Cabau

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 19, 2023

Cyathostomins are a complex of 50 intestinal parasite species infecting horses and wild equids. The massive administration modern anthelmintic drugs has increased their relative abundance in horse helminth communities selected drug-resistant isolates worldwide. Cylicocyclus nassatus is the most prevalent abundant species. tedious identification isolation these worms have hampered studies biology that remain largely uncharacterised. Here we leveraged ultra-low input sequencing protocols to build reference genome for strongyle Using this resource, established first estimates its genetic diversity population structure on gradient ranging from Ukraine (close domestication area) North America, while capturing 19th-century snapshot C. Egypt. Our results support diverse lowly structured global population. Modern populations displayed lower nucleotide old African isolate. We identified candidates upon which pyrantel (an drug used companion animals) selection likely applied field populations, highlighting previously suspected genes coding nicotinic acetylcholine receptor subunits, identifying new showing differential expression independently evolved Caenorhabditis elegans lines. These offer resource widen current knowledge cyathostomin biology, unravel novel aspects resistance mechanisms provide candidate track field.

Language: Английский

Citations

Identification of third stage larvae of strongyles and molecular diagnosis of Strongylus vulgaris in the feces of Thoroughbred horses kept in training centers in Rio de Janeiro, Brazil DOI

André Vianna Martins, Laís Lisboa Corrêa,

Mariana Santos Ribeiro

et al.

Veterinary Parasitology Regional Studies and Reports, Journal Year: 2024, Volume and Issue: 50, P. 101019 - 101019

Published: April 3, 2024

Language: Английский

Citations

Changes in equine strongylid communities after two decades of annual anthelmintic treatments at the farm level DOI

Тetiana А. Kuzmina, Alžbeta Königová, Anatoliy Antipov

et al.

Parasitology Research, Journal Year: 2024, Volume and Issue: 123(11)

Published: Nov. 1, 2024

Language: Английский

Citations