Gut bacterial communities in roadkill animals: A pioneering study of two species in the Amazon region in Ecuador DOI Creative Commons
Manuel Alejandro Coba-Males, Magdalena Díaz, C. Alfonso Molina

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0313263 - e0313263

Published: Dec. 30, 2024

Studying the microbial communities within gastrointestinal tract of vertebrate species can provide insights into biodiversity, disease ecology, and conservation. Currently, we have very limited understanding composition endogenous microbiota in wildlife, particularly high biodiversity tropical areas. Knowledge is by logistical ethical challenges obtaining samples for free-living animals. Roadkill carcasses offer a largely untapped source biological material, including gut microbiota. These animals that died on roads due to collisions with vehicles are suitable accessible, opportunistic sampling. Here, used metabarcoding V 3 —V 4 region 16S rRNA gene nine roadkill collected from road Ecuador representing two species: speckled worm lizard ( Amphisbaena bassleri ) smooth-billed ani Crotophaga ). We successfully identify phyla both Firmicutes, Bacteroidetes, Proteobacteria A . , Firmicutes Actinobacteria C Our study provides first description these vertebrates, demonstrates feasibility studying material be opportunistically preserved biobanks.

Language: Английский

Seasonal dietary changes relate to gut microbiota composition depending on the host species but do not correlate with gut microbiota diversity in arthropod‐eating lizards DOI Creative Commons
Mauricio Hernández, Sergio Ancona, Stephanie Hereira‐Pacheco

et al.

Molecular Ecology, Journal Year: 2024, Volume and Issue: 33(14)

Published: June 2, 2024

Abstract The animal gut microbiota is strongly influenced by environmental factors that shape their temporal dynamics. Although diet recognized as a major driver of variation, dietary patterns have seldom been linked to dynamics in wild animals. Here, we analysed the variation between dry and rainy seasons across four Sceloporus species ( S. aeneus , bicanthalis grammicus spinosus ) from central Mexico light changes composition. lizard was dominated Firmicutes (now Bacillota) Bacteroidota, closely related shared great number core bacterial taxa. We report species‐specific seasonal diversity composition: greater alpha during compared season opposite pattern no differences . Our findings indicated positive association composition for but did not increase linearly with richness any species. In addition, seasonality affected composition, microbial community similarity increased well Together, our results illustrate play role shaping populations, this rule other ecological influence variation.

Language: Английский

Citations

4
Anthropogenic reverberations on the gut microbiome of dwarf chameleons (Bradypodion) DOI Creative Commons
Matthew G. Adair, Krystal A. Tolley, Bettine Jansen van Vuuren

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18811 - e18811

Published: Feb. 28, 2025

Exploration of the microbiome has been referred to as a final frontier in biological research. This is due its precedence for generating insights on holistic functioning organismal biology by exploring interactions between hosts and their associated symbiotic organisms. The microbiomes many vertebrate groups still require exploration advance current knowledge fill previous gaps. study generated initial descriptions bacterial three species dwarf chameleon ( Bradypodion ) from 16S rRNA gene region targeting V3 V4 hypervariable regions. led successful identification 1,073 4,502 independent amplicon sequence variants buccal swab faecal material samples, respectively. newly acquired information intended baseline future work incorporating holobiont information. diversity microbial taxa suggests that total similar other squamates investigated date, well chelonians (Testudines). Microbial frequency differences were noted comparison crocodilians (Archosauria) mammalian groups. Furthermore, this aimed examine influence habitat transformation composition chameleons each occupy both urban natural habitats. Given most habitats are highly transformed, expectation was assemblages gastro-intestinal tracts all would show significant populations i.e. , natural, or urban). It found, however, level effect contingent species: B. melanocephalum showed noticeable populations; thamnobates variations community dispersions setaroi no based metrics although some differences, composition, observed populations. We suggest magnitude difference occupied factor, given apparent disparity compared two species.

Language: Английский

Citations

0
Blood and cloacal microbiome profile of captive green turtles (Chelonia mydas) and hawksbill turtles (Eretmochelys imbricata): Water quality and conservation implications DOI

Xin Li Ching,

Syamsyahidah Samsol,

Mohd Uzair Rusli

et al.

Chemosphere, Journal Year: 2025, Volume and Issue: 375, P. 144223 - 144223

Published: March 5, 2025

Language: Английский

Citations

0
16S rRNA Gene Sequencing-Based Identification and Comparative Analysis of the Fecal Microbiota of Five Syntopic Lizard Species from a Low-Mountain Area in Western Bulgaria DOI Creative Commons
Irina Lazarkevich, Stephan Engıbarov, Simona Mitova

et al.

Applied Microbiology, Journal Year: 2024, Volume and Issue: 4(1), P. 181 - 193

Published: Jan. 18, 2024

Studies on the gut microbiome of free-living reptiles in Europe are generally fragmentary and still missing Bulgaria. We aimed to identify compare fecal microbiota profiles five syntopic lizard species from three families: European green (Lacerta viridis), common wall (Podarcis muralis), meadow (Darevskia praticola) (Lacertidae), snake-eyed skink (Ablepharus kitaibelii) (Scincidae), slow worm (Anguis fragilis) (Anguidae), which coinhabit a low mountainous area western part country. A high-throughput sequencing hypervariable V3-V4 region 16S rRNA gene, performed Illumina HiSeq2500 platform, was used. The core hosts seems be species-specific. dynamic phyla proportion between found. richest alpha diversity observed D. praticola, lowest P. muralis A. fragilis. Within lacertids, praticola L. viridis were more closely related each other than they those muralis. Sharing largely trophic resource (all except fragilis mainly insectivorous) not an indication similarity their microbial communities.

Language: Английский

Citations

2
Comparative gut microbiome research through the lens of ecology: theoretical considerations and best practices DOI Creative Commons
Samuel Degregori, Xiaolin Wang,

Akhil Kommala

et al.

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Comparative approaches in animal gut microbiome research have revealed patterns of phylosymbiosis, dietary and physiological convergences, environment-host interactions. However, most large-scale comparative studies, especially those that are highly cited, focused on mammals, efforts to integrate with existing ecological frameworks lacking. While mammals serve as useful model organisms, developing generalised principles how microbiomes shaped these interact bidirectionally host ecology evolution requires a more complete sampling the kingdom. Here, we provide an overview what past studies taught us about microbiome, community theory may help resolve certain contradictions research. We explore whether hypotheses supported across clades, disproportionate focus has introduced potential bias into theory. then introduce methodological solution by which public data understudied hosts can be compiled analysed context. Our aggregation analysis 179 shows generating sets rich diversity is possible key microbes associated widespread also show effects sample size taxonomic rank results multivariate analyses vary significantly two parameters. challenges remain universal bring one step closer integrating evolution.

Language: Английский

Citations

1
Gut bacterial communities in roadkill animals: A pioneering study of two species in the Amazon region in Ecuador DOI Creative Commons
Manuel Alejandro Coba-Males, Magdalena Díaz, C. Alfonso Molina

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0313263 - e0313263

Published: Dec. 30, 2024

Studying the microbial communities within gastrointestinal tract of vertebrate species can provide insights into biodiversity, disease ecology, and conservation. Currently, we have very limited understanding composition endogenous microbiota in wildlife, particularly high biodiversity tropical areas. Knowledge is by logistical ethical challenges obtaining samples for free-living animals. Roadkill carcasses offer a largely untapped source biological material, including gut microbiota. These animals that died on roads due to collisions with vehicles are suitable accessible, opportunistic sampling. Here, used metabarcoding V 3 —V 4 region 16S rRNA gene nine roadkill collected from road Ecuador representing two species: speckled worm lizard ( Amphisbaena bassleri ) smooth-billed ani Crotophaga ). We successfully identify phyla both Firmicutes, Bacteroidetes, Proteobacteria A . , Firmicutes Actinobacteria C Our study provides first description these vertebrates, demonstrates feasibility studying material be opportunistically preserved biobanks.

Language: Английский

Citations

0