Targeted regulation of miR-154-5p/Cullin2 pathway by hsa_circ_TRIM22 in promoting human papillomavirus 16 positive cervical cancer progression

Journal of Cancer, Journal Year: 2024, Volume and Issue: 15(8), P. 2137 - 2146

Published: Jan. 1, 2024

Background.Tripartite motif-containing 22 (TRIM22) is characterized by a canonical RING domain with ubiquitin E3 ligase activity and closely associated tumorigenesis.As product of TRIM22 transcription, whether hsa_circ_TRIM22 has function regulating tumorigenesis unclear.Thus, we aimed to explore the role mechanism in human papillomavirus (HPV) 16 positive cervical cancer (CC).Methods.We collected HPV16-positive tissues including chronic cervicitis, high-grade squamous intraepithelial lesions (HSIL), low-grade (LSIL), CC, along CC cell lines detect level using real-time fluorescence quantitative polymerase chain reaction (RT-qPCR).Hsa_circ_TRIM22 was silenced specific short hairpin ribonucleic acid (shRNA) functional assays were performed thereafter.Mechanistically, targeting regulatory relationship between miR-154-5p confirmed luciferase report assay rescue experiments.Results.We found expression significantly higher cells tissues.Further, knockdown inhibited migration, proliferation, invasiveness, enhanced apoptosis, slowed cycle.Mechanistically, could bind prevent from reducing levels Cullin2 (CUL2).Notably, application inhibitor rescued hsa_circ_TRIM22-mediated tumorigenesis.Conclusions.Our observations suggest upregulated promotes progression miR-154-5p/CUL2 axis, thereby serving as promising candidate for diagnosis treatments CC.

Language: Английский

MicroRNAs, long non-coding RNAs, and circular RNAs and gynecological cancers: focus on metastasis

Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13

Published: Oct. 3, 2023

Gynecologic cancer is a significant cause of death in women worldwide, with cervical cancer, ovarian and endometrial being among the most well-known types. The initiation progression gynecologic cancers involve variety biological functions, including angiogenesis metastasis—given that mostly occurs from metastatic tumors have invaded surrounding tissues. Therefore, understanding molecular pathways underlying metastasis critical for enhancing patient survival outcomes. Recent research has revealed contribution numerous non-coding RNAs (ncRNAs) to invasion by affecting specific cellular pathways. This review focuses on three types (ovarian, endometrial, cervical) kinds ncRNAs (long RNAs, microRNAs, circular RNAs). We summarize detailed role different interactions involved these cancers.

Language: Английский