Evaluating the coral microbiome during cryopreservation DOI Creative Commons

Tori Jefferson,

E. Michael Henley, Patrick M. Erwin

et al.

Cryobiology, Journal Year: 2024, Volume and Issue: 117, P. 104960 - 104960

Published: Sept. 17, 2024

Coral reefs are threatened by various local and global stressors, including elevated ocean temperatures due to anthropogenic climate change. cryopreservation could help secure the diversity of corals. Recently, isochoric vitrification was used demonstrate that coral fragments lived 24 hr post-thaw; however, in this study, they were stressed post-thaw. The microbial portion holobiont has been shown affect host fitness impact treatment on microbiomes is unknown. Therefore, we examined coral-associated bacterial communities pre- post-cryopreservation treatments, with a view towards informing potential future stress reduction strategies. We characterized microbiome Hawaiian finger coral, Porites compressa wild at seven steps during process. observed significant changes composition, including: 1) natural P. dominated Endozoicomonadaceae (76.5 % relative abundance) consistent between samples, independent collection location across Kāne'ohe Bay; 2) reduced <6.9 captivity, further <0.5 abundance after vitrification; 3) Vibrionaceae post-thaw (58.5-74.7 abundance). Thus, capture processes, implicated as possible causal agents dysbiosis loss putatively beneficial symbionts (Endozoicomonadaceae) overgrowth pathogens (Vibrionaceae). Offsetting these probiotic restoration treatments may alleviate improve husbandry.

Language: Английский

Evaluating the coral microbiome during cryopreservation DOI Creative Commons

Tori Jefferson,

E. Michael Henley, Patrick M. Erwin

et al.

Cryobiology, Journal Year: 2024, Volume and Issue: 117, P. 104960 - 104960

Published: Sept. 17, 2024

Coral reefs are threatened by various local and global stressors, including elevated ocean temperatures due to anthropogenic climate change. cryopreservation could help secure the diversity of corals. Recently, isochoric vitrification was used demonstrate that coral fragments lived 24 hr post-thaw; however, in this study, they were stressed post-thaw. The microbial portion holobiont has been shown affect host fitness impact treatment on microbiomes is unknown. Therefore, we examined coral-associated bacterial communities pre- post-cryopreservation treatments, with a view towards informing potential future stress reduction strategies. We characterized microbiome Hawaiian finger coral, Porites compressa wild at seven steps during process. observed significant changes composition, including: 1) natural P. dominated Endozoicomonadaceae (76.5 % relative abundance) consistent between samples, independent collection location across Kāne'ohe Bay; 2) reduced <6.9 captivity, further <0.5 abundance after vitrification; 3) Vibrionaceae post-thaw (58.5-74.7 abundance). Thus, capture processes, implicated as possible causal agents dysbiosis loss putatively beneficial symbionts (Endozoicomonadaceae) overgrowth pathogens (Vibrionaceae). Offsetting these probiotic restoration treatments may alleviate improve husbandry.

Language: Английский

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