Knockout of B2M in combination with PD-L1 overexpression protects MSC-derived new islet β cells from graft rejection in the treatment of canine diabetes mellitus DOI Creative Commons
Pengxiu Dai, Yi Wu, Qingjie Du

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 2, 2024

The immunogenicity of allogeneic mesenchymal stem cells (MSCs) is significantly enhanced after transplantation or differentiation, and these can be recognized cleared by recipient immune cells. Graft rejection has become a major obstacle to improving the therapeutic effect MSCs or, their in treatment diabetes other diseases. Solving this problem helpful for prolonging time that play role body clinical effect. In study, canine adipose-derived (ADSCs) were used as seed cells, gene editing technology was knock out B2M cooperate with overexpression PD-L1 gene. Gene-edited ADSCs (GeADSCs), whose biological characteristics safety are not different from those normal ADSCs, have been obtained. GeADSCs reduced, escape ability enhanced, remain longer time. Using optimized induction program, efficiency differentiation into new islet β-cells increased, maturity increased. decreased, transplanted diabetic dogs (the graft site prevascularized implantation scaffold form vascularized pouch). number infiltrating content factors decreased at site. New β-cell transplantation, which low immunogenicity, reverse dogs, cell enhanced. This study provides method survival functional transplant recipients

Language: Английский

Prognostic significance of neutrophil extracellular trap-related genes in childhood acute lymphoblastic leukemia: insights from multi-omics and in vitro experiment DOI Creative Commons
Cheng Chen, Yu Ma,

Ya-chen Gao

et al.

Hematology, Journal Year: 2025, Volume and Issue: 30(1)

Published: Jan. 20, 2025

Background This study aimed to develop a prognostic model based on extracellular trap-related genes (NETRGs) for patients with cALL.

Language: Английский

Citations

0
Mapping Cell Identity from scRNA-seq: a primer on computational methods DOI Creative Commons
Daniele Traversa, Matteo Chiara

Computational and Structural Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Single cell (sc) technologies mark a conceptual and methodological breakthrough in our way to study cells, the base units of life. Thanks these technological developments, large-scale initiatives are currently ongoing aimed at mapping all types human body, with ambitious aim gain cell-level resolution physiological development disease. Since its broad applicability ease interpretation scRNA-seq is probably most common sc-based application. This assay uses high throughput RNA sequencing capture gene expression profiles sc-level. Subsequently, under assumption that differences transcriptional programs correspond distinct cellular identities, ad-hoc computational methods used infer from patterns. A wide array were developed for this task. However, depending on underlying algorithmic approach associated requirements, each method might have specific range application, implications not always clear end user. Here we will provide concise overview state-of-the-art identity annotation scRNA-seq, tailored new users non-computational scientists. To end, classify existing tools five main categories, discuss their key strengths, limitations

Language: Английский

Citations

0
Emerging Topics and Future Directions DOI
Khalid Raza

SpringerBriefs in applied sciences and technology, Journal Year: 2024, Volume and Issue: unknown, P. 81 - 88

Published: Jan. 1, 2024

Language: Английский

Citations

0
Knockout of B2M in combination with PD-L1 overexpression protects MSC-derived new islet β cells from graft rejection in the treatment of canine diabetes mellitus DOI Creative Commons
Pengxiu Dai, Yi Wu, Qingjie Du

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 2, 2024

The immunogenicity of allogeneic mesenchymal stem cells (MSCs) is significantly enhanced after transplantation or differentiation, and these can be recognized cleared by recipient immune cells. Graft rejection has become a major obstacle to improving the therapeutic effect MSCs or, their in treatment diabetes other diseases. Solving this problem helpful for prolonging time that play role body clinical effect. In study, canine adipose-derived (ADSCs) were used as seed cells, gene editing technology was knock out B2M cooperate with overexpression PD-L1 gene. Gene-edited ADSCs (GeADSCs), whose biological characteristics safety are not different from those normal ADSCs, have been obtained. GeADSCs reduced, escape ability enhanced, remain longer time. Using optimized induction program, efficiency differentiation into new islet β-cells increased, maturity increased. decreased, transplanted diabetic dogs (the graft site prevascularized implantation scaffold form vascularized pouch). number infiltrating content factors decreased at site. New β-cell transplantation, which low immunogenicity, reverse dogs, cell enhanced. This study provides method survival functional transplant recipients

Language: Английский

Citations

0