Enhancing Laccase Production by Trametes hirsuta GMA-01 Using Response Surface Methodology and Orange Waste: A Novel Breakthrough in Sugarcane Bagasse Saccharification and Synthetic Dye Decolorization DOI Creative Commons

Guilherme Guimarães Ortolan,

Alex Graça Contato, Guilherme Mauro Aranha

et al.

Reactions, Journal Year: 2024, Volume and Issue: 5(3), P. 635 - 650

Published: Sept. 19, 2024

Trametes hirsuta GMA-01 was cultivated in a culture medium supplemented with orange waste, starch, wheat bran, yeast extract, and salts. The fungus produced several holoenzymes, but the laccase levels were surprisingly high. Given highlighted applicability of laccases various biotechnological areas minimal environmental impact, we provided strategy to increase its production using response surface methodology. immobilization into ionic supports (CM-cellulose, DEAE-agarose, DEAE-cellulose, DEAE-Sephacel, MANAE-agarose, MANAE-cellulose, PEI-agarose) found be efficient recuperative, showcasing technical prowess research. crude extract (CE) CM-cellulose-immobilized (ICE) showed optimum activity acidic conditions (pH 3.0) at 70 °C for CE 60 ICE. ICE significantly increased thermostability which retained 21.6% residual after 240 min. successfully applied sugarcane bagasse hydrolysis, showing 13.83 ± 0.02 µmol mL−1 reducing sugars 48 h. Furthermore, tested dye decolorization, achieving 96.6%, 71.9%, 70.8% decolorization bromocresol green, bromophenol blue, orcein, respectively (0.05% (w/v) concentration). properties versatility T. different purposes are interesting notable, opening potential applications providing valuable insights future development.

Language: Английский

Metal–organic frameworks in drug delivery: engineering versatile platforms for therapeutic applications DOI Creative Commons
Doaa S. R. Khafaga,

Manar T. El-Morsy,

Habiba Faried

et al.

RSC Advances, Journal Year: 2024, Volume and Issue: 14(41), P. 30201 - 30229

Published: Jan. 1, 2024

Recently, metal-organic frameworks (MOFs) have attracted much attention as versatile materials for drug delivery and personalized medicine. MOFs are porous structures made up of metal ions coupled with organic ligands. This review highlights the synthesis techniques used to design specific features such surface area pore size, encapsulation within not only improves their stability solubility but also allows controlled release kinetics, which therapeutic efficacy minimizes adverse effects. Furthermore, it discusses challenges potential advantages MOF-based delivery, MOF stability, biocompatibility, scale-up production. With further advancements in synthesis, functionalization techniques, understanding interactions using biological systems, can significant promise expanding medicine improving patient outcomes.

Language: Английский

Citations

18

Characterization of a purified novel Aureobasidium pullulans NAC8 lipase and covalent-immobilization for use in the biodegradation of oil-contaminated wastewater DOI
Adedeji Nelson Ademakinwa, Zainab Adenike Ayinla,

Agunbiade Mayowa Oladele

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140781 - 140781

Published: Feb. 1, 2025

Language: Английский

Citations

1

Green chemistry: Modern therapies using nanocarriers for treating rare brain cancer metastasis from colon cancer DOI Creative Commons
Doaa S. R. Khafaga, Ghazala Muteeb,

Darin W Aswa

et al.

SLAS DISCOVERY, Journal Year: 2025, Volume and Issue: unknown, P. 100213 - 100213

Published: Jan. 1, 2025

Brain metastasis (BM) from colon cancer is associated with a poor prognosis and restricted treatment alternatives, largely due to issues related blood-brain barrier (BBB) permeability the negative effects of standard chemotherapy. Nanotechnology improves efficacy by enabling targeted controlled drug delivery. This review article evaluates potential nanotechnology-based therapies for treating BM, emphasizing their capacity cross BBB, diminish metastatic growth, enhance overall survival rates. A multiple studies evaluated nanoparticles (NPs) as carriers chemotherapy, focusing on parameters including particle size, surface charge, drug-loading capacity. The study also reviewed that examined BBB penetration, in vitro tumor accumulation, vivo growth inhibition. In findings indicated NPs accumulate more efficiently BM tissue than healthy brain show significant penetration. vivo, nanotherapy markedly inhibited prolonged relative conventional chemotherapy or control treatments while exhibiting reduced side effects. Recent demonstrated plant extracts can effectively safely synthesize nanomaterials, positioning them viable environmentally friendly precursor nanomaterial production. Nanotechnology-based demonstrate minimizing systemic toxicity, enhancing therapeutic efficacy, facilitating Further research required confirm these implement clinical practice.

Language: Английский

Citations

0

Magnetizing Biotech–Advances in (In Vivo) Magnetic Enzyme Immobilization DOI Creative Commons
Gizem Ölçücü, Karl‐Erich Jaeger, Ulrich Krauß

et al.

Engineering in Life Sciences, Journal Year: 2025, Volume and Issue: 25(3)

Published: March 1, 2025

ABSTRACT Industrial biocatalysis, a multibillion dollar industry, relies on the selectivity and efficacy of enzymes for efficient chemical transformations. However, enzymes, evolutionary adapted to mild biological conditions, often struggle in industrial processes that require harsh reaction resulting reduced stability activity. Enzyme immobilization, which addresses challenges such as enzyme reuse stability, has therefore become vital strategy improving use applications. Traditional immobilization techniques rely confinement or display within/on organic inorganic supports, while recent advances synthetic biology have led development solely vivo methods streamline production immobilization. These offer added benefits terms sustainability cost efficiency. In addition, multifunctional materials, magnetic (nano)materials enabled improved separation purification processes. The combination both “worlds,” opens up new avenues (industrial) fundamental science, biomedicine. Therefore, this review, we provide an overview established recently emerging generation protein immobilizates, placing special focus solutions.

Language: Английский

Citations

0

Self-assembled Immobilization and Metal-Polyphenol Network Encapsulation of β-Galactosidase on T4 phage for Enhanced Biocatalytic Performance. DOI
Dan Wu, Saba Khan, Shujie Zhang

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Abstract Enzymes, key catalysts in biochemical reactions, are prone to denaturation under harsh conditions, leading reduced stability and higher costs. Enzyme immobilization, using carriers like magnetic nanoparticles, metal-organic frameworks, viruses, is a common solution. T4 bacteriophage, virulent E. coli phage containing 155 Hoc 870 Soc proteins, offers cost-effective highly stable platform for enzyme immobilization. In this study, Soc-β-galactosidase (Soc-β-gal) was immobilized on the surface of bacteriophage via affinity fixation further encapsulated with metal-polyphenol network (MPN) coating. Comparative analysis properties revealed that enzyme, β-gal T4, retained over 85% activity after 6 hours at 50°C, while free Soc-β-gal only 40.63%. Moreover, T4@TA-Ti demonstrated superior stability, retaining 92.88% its UV exposure, compared 10.21% 7.23% Soc-β-gal. The MPN coating also enhanced resistance proteolytic degradation, 9.48% exposure proteinase K, contrast 4.62% T4. Overall, these results demonstrate immobilization significantly enhances improves extreme pH, ultraviolet radiation, other environmental stressors, highlighting potential approach biocatalytic applications.

Language: Английский

Citations

0

Enhancing Laccase Production by Trametes hirsuta GMA-01 Using Response Surface Methodology and Orange Waste: A Novel Breakthrough in Sugarcane Bagasse Saccharification and Synthetic Dye Decolorization DOI Creative Commons

Guilherme Guimarães Ortolan,

Alex Graça Contato, Guilherme Mauro Aranha

et al.

Reactions, Journal Year: 2024, Volume and Issue: 5(3), P. 635 - 650

Published: Sept. 19, 2024

Trametes hirsuta GMA-01 was cultivated in a culture medium supplemented with orange waste, starch, wheat bran, yeast extract, and salts. The fungus produced several holoenzymes, but the laccase levels were surprisingly high. Given highlighted applicability of laccases various biotechnological areas minimal environmental impact, we provided strategy to increase its production using response surface methodology. immobilization into ionic supports (CM-cellulose, DEAE-agarose, DEAE-cellulose, DEAE-Sephacel, MANAE-agarose, MANAE-cellulose, PEI-agarose) found be efficient recuperative, showcasing technical prowess research. crude extract (CE) CM-cellulose-immobilized (ICE) showed optimum activity acidic conditions (pH 3.0) at 70 °C for CE 60 ICE. ICE significantly increased thermostability which retained 21.6% residual after 240 min. successfully applied sugarcane bagasse hydrolysis, showing 13.83 ± 0.02 µmol mL−1 reducing sugars 48 h. Furthermore, tested dye decolorization, achieving 96.6%, 71.9%, 70.8% decolorization bromocresol green, bromophenol blue, orcein, respectively (0.05% (w/v) concentration). properties versatility T. different purposes are interesting notable, opening potential applications providing valuable insights future development.

Language: Английский

Citations

1