Definition of Synovial Mesenchymal Stem Cells for Meniscus Regeneration by the Mechanism of Action and General Amp1200 Gene Expression DOI Open Access
Kentaro Nakamura, Tsukasa Kitahashi, Ryo Kogawa

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10510 - 10510

Published: Sept. 29, 2024

The quality control (QC) of pharmaceutical-grade cell-therapy products, such as mesenchymal stem cells (MSCs), is challenging. Attempts to develop products have been hampered by difficulties defining cell-type-specific characteristics and therapeutic mechanisms action (MoAs). Although we developed a cell therapy product, FF-31501, consisting human synovial MSCs (SyMSCs), it was difficult find specific markers for SyMSCs define the separately from other MSCs. purpose this study create method identifying tissue-derived delve deeper into mechanism SyMSC-induced meniscus regeneration. Specifically, cell-type-dependent approach, constructed set 1143 genes (Amp1200) reported be associated with established evaluate them correlating gene expression patterns. As result, possible non-MSCs. In addition, analysis also highlighted TNSF-15. in vivo rat model injury found TNSF-15 an essential molecule regeneration via SyMSC administration. This previously MoA molecules allowed MoA-dependent approach SyMSCs. Therefore, were defined means two approaches: separate identification molecules. These approaches would useful QC products.

Language: Английский

Mesenchymal Stem Cell Treatment for Peripheral Nerve Injuries DOI
Yue Wu, Ruijie Lu,

Hongtao Yin

et al.

Journal of Cellular Physiology, Journal Year: 2025, Volume and Issue: 240(4)

Published: April 1, 2025

ABSTRACT Peripheral nerve injuries (PNI) affect hundreds of thousands patients annually, often leading to life‐altering consequences such as significant impairments in motor function and sensory perception. In recent years, a growing body evidence indicates that mesenchymal stem cell (MSC) treatment could complement traditional improve therapeutic outcomes for these injuries. This paper reviews emerging insights into the potential benefits MSC PNI summarizes selected examples interactions between MSCs, peripheral nerves, their microenvironment, which have advanced our understanding pathophysiology MSC‐based therapy. We believe this rapidly moving field holds great promise future advancements, guiding rational design safe effective treatments with PNI.

Language: Английский

Citations

0
The influence of cell source on the senescence of human mesenchymal stem/stromal cells DOI
Seyed Mehdi Hoseini, Fateme Montazeri

Human Cell, Journal Year: 2025, Volume and Issue: 38(3)

Published: April 12, 2025

Language: Английский

Citations

0
Definition of Synovial Mesenchymal Stem Cells for Meniscus Regeneration by the Mechanism of Action and General Amp1200 Gene Expression DOI Open Access
Kentaro Nakamura, Tsukasa Kitahashi, Ryo Kogawa

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10510 - 10510

Published: Sept. 29, 2024

The quality control (QC) of pharmaceutical-grade cell-therapy products, such as mesenchymal stem cells (MSCs), is challenging. Attempts to develop products have been hampered by difficulties defining cell-type-specific characteristics and therapeutic mechanisms action (MoAs). Although we developed a cell therapy product, FF-31501, consisting human synovial MSCs (SyMSCs), it was difficult find specific markers for SyMSCs define the separately from other MSCs. purpose this study create method identifying tissue-derived delve deeper into mechanism SyMSC-induced meniscus regeneration. Specifically, cell-type-dependent approach, constructed set 1143 genes (Amp1200) reported be associated with established evaluate them correlating gene expression patterns. As result, possible non-MSCs. In addition, analysis also highlighted TNSF-15. in vivo rat model injury found TNSF-15 an essential molecule regeneration via SyMSC administration. This previously MoA molecules allowed MoA-dependent approach SyMSCs. Therefore, were defined means two approaches: separate identification molecules. These approaches would useful QC products.

Language: Английский

Citations

0