Watkins wheat landraces decode nitrogen-driven biomass trade-offs: GWAS exposes root-shoot dialectics and elite landraces for resilient agriculture DOI Creative Commons
Abdul Waheed,

Muhammad Shahid Iqbal,

Zareen Sarfraz

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: May 23, 2025

Introduction Nitrogen limitation is a critical abiotic stressor that disrupts the balance between plants and their environment, imposing trade-offs in biomass allocation threaten crop productivity food security. While modern breeding programs often focus on improving shoot performance, genetic mechanisms coordinate root-shoot responses under nitrogen stress remain poorly understood. This study aimed to dissect molecular physiological foundations of nitrogen-driven resilience wheat, leveraging genetically diverse Watkins wheat landraces as source adaptive alleles. Methods A total 308 were phenotyped low (LN) normal (NN) conditions assess strategies. Genome-wide association studies (GWAS) conducted identify candidate genes governing nitrogen-responsive traits. Functional annotation transcriptomic validation used elucidate gene networks, haplotype mapping was employed link allelic variation geographic adaptation. Multivariate analysis performed classify strategies among landraces. Results Phenotypic revealed stark differences LN NN conditions. GWAS identified 130 genes, including root-specific RALF33 shoot-prioritizing TaNAR1 , involved highlighted antagonistic such TAF6 TaAPY6 balancing root meristem activity Adaptive alleles European optimized proliferation LN, while Eurasian exhibited shoot-root coordination through variants. classified into four distinct strategies, identifying elite genotypes resilient limitation. Discussion By integrating genomics, phenomics, mapping, this connects underlying nutrient with ecophysiological Key emerged actionable targets for marker-assisted develop nitrogen-efficient varieties. These findings highlight potential evolutionary-informed genetics enhance resilience, providing roadmap sustainable design context global scarcity.

Language: Английский

Watkins wheat landraces decode nitrogen-driven biomass trade-offs: GWAS exposes root-shoot dialectics and elite landraces for resilient agriculture DOI Creative Commons
Abdul Waheed,

Muhammad Shahid Iqbal,

Zareen Sarfraz

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: May 23, 2025

Introduction Nitrogen limitation is a critical abiotic stressor that disrupts the balance between plants and their environment, imposing trade-offs in biomass allocation threaten crop productivity food security. While modern breeding programs often focus on improving shoot performance, genetic mechanisms coordinate root-shoot responses under nitrogen stress remain poorly understood. This study aimed to dissect molecular physiological foundations of nitrogen-driven resilience wheat, leveraging genetically diverse Watkins wheat landraces as source adaptive alleles. Methods A total 308 were phenotyped low (LN) normal (NN) conditions assess strategies. Genome-wide association studies (GWAS) conducted identify candidate genes governing nitrogen-responsive traits. Functional annotation transcriptomic validation used elucidate gene networks, haplotype mapping was employed link allelic variation geographic adaptation. Multivariate analysis performed classify strategies among landraces. Results Phenotypic revealed stark differences LN NN conditions. GWAS identified 130 genes, including root-specific RALF33 shoot-prioritizing TaNAR1 , involved highlighted antagonistic such TAF6 TaAPY6 balancing root meristem activity Adaptive alleles European optimized proliferation LN, while Eurasian exhibited shoot-root coordination through variants. classified into four distinct strategies, identifying elite genotypes resilient limitation. Discussion By integrating genomics, phenomics, mapping, this connects underlying nutrient with ecophysiological Key emerged actionable targets for marker-assisted develop nitrogen-efficient varieties. These findings highlight potential evolutionary-informed genetics enhance resilience, providing roadmap sustainable design context global scarcity.

Language: Английский

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