Regenerative Medicine in Plastic Surgery: The Role of Stem Cells and Bioprinting
Regenesis repair rehabilitation.,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 1, 2025
Language: Английский
Development of Biomimetic Substrates for Limbal Epithelial Stem Cells Using Collagen-Based Films, Hyaluronic Acid, Immortalized Cells, and Macromolecular Crowding
Life,
Journal Year:
2024,
Volume and Issue:
14(12), P. 1552 - 1552
Published: Nov. 26, 2024
Despite
the
promising
potential
of
cell-based
therapies
developed
using
tissue
engineering
techniques
to
treat
a
wide
range
diseases,
including
limbal
stem
cell
deficiency
(LSCD),
which
leads
corneal
blindness,
their
commercialization
remains
constrained.
This
is
primarily
attributable
limited
sources,
use
non-standardizable,
unscalable,
and
unsustainable
techniques,
extended
manufacturing
processes
required
produce
transplantable
tissue-like
surrogates.
Herein,
we
present
first
demonstration
novel
approach
combining
collagen
films
(CF),
hyaluronic
acid
(HA),
human
telomerase-immortalized
epithelial
cells
(T-LESCs),
macromolecular
crowding
(MMC)
develop
innovative
biomimetic
substrates
for
(LESCs).
The
initial
step
involved
fabrication
characterization
CF
enriched
with
HA
(CF-HA).
Subsequently,
T-LESCs
were
seeded
on
CF,
CF-HA,
culture
plastic
(TCP).
Thereafter,
effect
these
matrices
basic
cellular
function
tissue-specific
extracellular
matrix
(ECM)
deposition
or
without
MMC
was
evaluated.
viability
metabolic
activity
cultured
TCP
found
be
similar,
while
CF-HA
induced
highest
(p
<
0.05)
proliferation.
It
notable
that
growth,
whereas
increased
IV,
fibronectin,
laminin
in
T-LESC
culture.
data
highlight
of,
particular,
immortalized
development
substrates,
could
utilized
ocular
surface
reconstruction
following
further
vitro,
vivo,
clinical
validation
approach.
Language: Английский
From Bench to Bedside: Translating Cellular Rejuvenation Therapies into Clinical Applications
Cells,
Journal Year:
2024,
Volume and Issue:
13(24), P. 2052 - 2052
Published: Dec. 12, 2024
Cellular
rejuvenation
therapies
represent
a
transformative
frontier
in
addressing
age-related
decline
and
extending
human
health
span.
By
targeting
fundamental
hallmarks
of
aging—such
as
genomic
instability,
epigenetic
alterations,
mitochondrial
dysfunction,
cellular
senescence—these
aim
to
restore
youthful
functionality
cells
tissues,
offering
new
hope
for
treating
degenerative
diseases.
Recent
advancements
have
showcased
range
strategies,
including
reprogramming,
senolytic
interventions,
restoration,
stem
cell-based
approaches,
gene-editing
technologies
like
CRISPR.
Each
modality
has
demonstrated
substantial
potential
preclinical
models
is
now
being
cautiously
explored
early-stage
clinical
trials.
However,
translating
these
from
the
laboratory
practice
presents
unique
challenges:
safety
concerns,
delivery
precision,
complex
regulatory
requirements,
ethical
considerations,
high
costs
impede
widespread
adoption.
This
review
examines
current
landscape
rejuvenation,
highlighting
key
advancements,
risks,
strategies
needed
overcome
hurdles.
Language: Английский
Stem Cells Within Three-Dimensional-Printed Scaffolds Facilitate Airway Mucosa and Bone Regeneration and Reconstruction of Maxillary Defects in Rabbits
Mi Hyun Lim,
No information about this author
Jung Ho Jeon,
No information about this author
Sun Hwa Park
No information about this author
et al.
Medicina,
Journal Year:
2024,
Volume and Issue:
60(12), P. 2111 - 2111
Published: Dec. 23, 2024
Background
and
Objectives:
Current
craniofacial
reconstruction
surgical
methods
have
limitations
because
they
involve
facial
deformation.
The
region
includes
many
areas
where
the
mucosa,
exposed
to
air,
is
closely
adjacent
bone,
with
maxilla
being
a
prominent
example
of
this
structure.
Therefore,
study
explored
whether
human
neural-crest-derived
stem
cells
(hNTSCs)
aid
bone
airway
mucosal
regeneration
during
using
rabbit
model.
Materials
Methods:
hNTSCs
were
induced
differentiate
into
either
epithelial
or
osteogenic
in
vitro.
seeded
polycaprolactone
scaffold
(three-dimensionally
printed)
that
implanted
rabbits
maxillary
defects.
Four
weeks
later,
tissue
was
analyzed
via
histological
evaluation
immunofluorescence
staining.
Results:
In
vitro,
differentiated
both
cells.
hNTSC
differentiation
respiratory
confirmed
by
Alcian
Blue
staining,
cilia
SEM,
increased
expression
levels
FOXJ1
E-cadherin
through
quantitative
RT-PCR.
Alizarin
Red
mRNA
BMP2
(6.1-fold)
RUNX2
(2.3-fold)
group
compared
control.
post-transplantation,
harvested,
H&E,
immunohistofluorescence
staining
performed.
H&E
SEM
showed
new
around
defect
more
group.
Also,
positive
for
acetylated
α-tubulin
cytokerin-5
control
Conclusions:
combined
PCL
enhanced
vitro
promoted
vivo
Language: Английский