Molecular identification of plasmid-mediated quinolone resistance genes among klebsiella pneumoniae clinical isolates from southwest Iran

BMC Infectious Diseases, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 14, 2025

In recent years, the resistance rate to quinolone antibiotics has been increasing in Klebsiella pneumoniae. Plasmid-mediated (PMQR) genes are one of factors causing quinolones. Hence, this study aimed determine incidence PMQR clinical K. pneumoniae isolates from southwest Iran. total, 96 were identified during period. Sixty-seven (69.8%) resistant at least antibiotic. Resistance rates levofloxacin, norfloxacin, nalidixic acid, ofloxacin, and ciprofloxacin 50% (48/96), 56.3% (54/96), 60.4% (58/96), 62.5% (60/96), 68.8% (66/96), respectively. The highest frequencies related qnrS, aac (6')-Ib-cr, qnrB with 85.1% (57/67), 83.6% (56/67) 65.7% (44/67), qnrD, qnrC, qepA, qnrA detected 23.9% (16/67), 19.4% (13/67), 13.4% (9/67), 6.0% (4/67) isolates, This was first report a high-frequency setting Ahvaz, As result, antibiotic prescribing policies should be amended hinder further spread studied region.

Language: Английский

Comprehensive Analysis of Virulence Genes, Antibiotic Resistance, Biofilm Formation, and Sequence Types in Clinical Isolates of Klebsiella pneumoniae

Canadian Journal of Infectious Diseases and Medical Microbiology, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

Background: The rise in multidrug‐resistant pathogens poses a formidable challenge treating hospital‐acquired infections, particularly those caused by Klebsiella pneumoniae . Biofilm formation is critical factor contributing to antibiotic resistance, enhancing bacterial adherence and persistence. K. strains vary virulence factors, influencing their pathogenicity resistance profiles. This study aimed comprehensively analyze patterns, biofilm clinical isolates of from Hamadan hospitals. Moreover, the explored molecular epidemiological relationships among using multilocus sequence typing (MLST) uncover genetic diversity associated with virulence. Materials Methods: Between December 2022 April 2024, 402 were collected samples, including urine, tracheal aspirates, blood, wounds, abscesses, teaching hospitals Hamadan. Initial culturing was performed on blood agar MacConkey agar, identified biochemical tests. Antimicrobial susceptibility testing followed CLSI, employing Kirby–Bauer disk diffusion method 10 antibiotics. assessed microtiter plate method, genes detected PCR. MLST analysis conducted selected based gene profiles patterns. Result: Of 456 analyzed, (88.15%) as , predominantly isolated samples (251/402, 62.44%), urine (105/402, 26.12%), (30/402, 7.46%), wounds (15/402, 3.73%), abscesses (1/402, 0.25%). Antibiotic rates revealed high cefepime (356/402, 88.55%), imipenem (345/402, 85.82%), ceftazidime (305/402, 75.87%), while amikacin (165/402, 41.04%) piperacillin–tazobactam (75/402, 18.65%) comparatively lower. varied isolates, 17/402 (4.22%) forming strong biofilms, 104/402 (25.87%) moderate 180/402 (44.78%) weak 101/402 (25.12%) showing no production. Virulence indicated prevalence for mrkD (396/402, 98.50%), fimH1 (351/402, 87.31%), entB (402/402, 100%), like irp-1 (151/402, 37.56%) irp-2 (136/402, 33.83%) less common, hylA cnf-1 absent. types ST147 (5/10, 50%), ST11 (3/10, 30%), ST15 (2/10, 20%). Conclusion: demonstrates notable biofilm‐associated supported significant association XDR strains, along diverse array underscores importance understanding epidemiology effective management hospital emphasizing need targeted surveillance infection control measures.

Language: Английский

Emerging Trends in Quinolone Resistance among Urinary Pathogens: A Brief Review

South Asian Journal of Research in Microbiology, Journal Year: 2024, Volume and Issue: 18(3), P. 32 - 38

Published: March 13, 2024

Urinary tract infections (UTIs) present a significant global health challenge, Intensified by the high level of antimicrobial resistance, particularly to quinolone antibiotics. This brief review synthesizes recent literature elucidate prevalence and mechanisms with focus on plasmid-mediated resistance (PMQR), among UTI-causing pathogens. Highlighting genetic basis including mutations in DNA gyrase efflux pump regulation genes, as well role such 'qnr' underscores clinical implications UTI management. Understanding these emerging trends is urgently required for improving management strategies guiding effective interventions control spread urinary

Language: Английский