Challenges Facing CRISPR/Cas9-Based Genome Editing in Plants

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: May 18, 2022

The development of plant varieties with desired traits is imperative to ensure future food security. revolution genome editing technologies based on the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9) system has ushered in a new era breeding. Cas9 and single-guide RNA (sgRNA) form an effective targeting complex locus or loci interest, enabling all plants high accuracy efficiency. Therefore, CRISPR/Cas9 can save both time labor relative what typically associated traditional breeding methods. However, despite improvements gene editing, several challenges remain that limit application CRISPR/Cas9-based plants. Here, we focus four issues relevant editing: (1) organelle editing; (2) transgene-free (3) virus-induced (4) recalcitrant elite crop inbred lines. This review provides up-to-date summary state CRISPR/Cas9-mediated will push this technique forward.

Language: Английский

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Combined Abiotic Stresses: Challenges and Potential for Crop Improvement

Agronomy, Journal Year: 2022, Volume and Issue: 12(11), P. 2795 - 2795

Published: Nov. 10, 2022

Abiotic stressors are major constraints that affect agricultural plant physio-morphological and biochemical attributes, resulting in a loss of normal functioning and, eventually, severe decline crop productivity. The co-occurrence different abiotic stresses, rather than specific stress situation, can alter or trigger wide range responses, such as altered metabolism, stunted growth, restricted development. Therefore, systematic rigorous studies pivotal for understanding the impact concurrent conditions on In doing so, this review emphasizes implications potential mechanisms controlling/managing combined which then be utilized to identify genotypes with tolerance. Furthermore, focuses recent biotechnological approaches deciphering tolerance plants. As result, agronomists, breeders, molecular biologists, field pathologists will benefit from literature assessing interactions between stresses performance development tolerant/resistant cultivars.

Language: Английский

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Advancement in the Breeding, Biotechnological and Genomic Tools towards Development of Durable Genetic Resistance against the Rice Blast Disease

Plants, Journal Year: 2022, Volume and Issue: 11(18), P. 2386 - 2386

Published: Sept. 13, 2022

Rice production needs to be sustained in the coming decades, as changeable climatic conditions are becoming more conducive disease outbreaks. The majority of rice diseases cause enormous economic damage and yield instability. Among them, blast caused by Magnaportheoryzae is a serious fungal considered one major threats world production. This pathogen can infect above-ground tissues plants at any growth stage causes complete crop failure under favorable conditions. Therefore, management essentially required sustain global food When looking drawback chemical strategy, development durable, resistant varieties most sustainable, economic, environment-friendly approaches counter outbreaks blasts. Interestingly, several blast-resistant cultivars have been developed with help breeding biotechnological methods. In addition, 146 R genes identified, 37 among them molecularly characterized date. Further, than 500 loci identified for resistance which enhances resources developing through marker-assisted selection (MAS), backcross (MABB), genome editing tools. Apart from these, better understanding pathogens, infection process pathogen, genetics immune response host plant very important effective disease. high throughput phenotyping screening protocols played significant roles easy comprehension mechanism spread. present review critically emphasizes pathogenesis, pathogenomics, techniques, traditional molecular approaches, transgenic tools develop broad spectrum durable against rice. updated comprehensive information presented this would definitely helpful researchers, breeders, students planning execution program pathogen.

Language: Английский

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Exploring molecular mechanisms, therapeutic strategies, and clinical manifestations of Huntington’s disease

Archives of Pharmacal Research, Journal Year: 2024, Volume and Issue: 47(6), P. 571 - 595

Published: May 19, 2024

Language: Английский

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CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing

Frontiers in Plant Science, Journal Year: 2016, Volume and Issue: 7

Published: Nov. 21, 2016

Genome editing advancements have made many unachievable ideas practical. Increased adoption of genome has been geared by swiftly developing CRISPR-Cas9 technology. This technique is appearing as driving force for innovative utilization in diverse branches plant biology. CRISPR mediated being used rapid, easy and efficient alteration indigenous genes among species. With approximate completion conceptual work about CRISPR/Cas9, scientists are applying this tool crop attributes enhancement. The capability systems performing targeted modifications sequence well gene expression will certainly spur novel developments not only model plants but also plants. Additionally, due to non-involvement foreign DNA, may help alleviating regulatory issues associated with GM Plants. We expect that prevailing challenges science like genomic region manipulation, specific vectors etc. be addressed along sustained growth tool. In review, recent progress CRISPR/Cas9 technology summarized discussed. review potential different aspects life. It covers strengths comparison other techniques e.g. ZFNs TALENs coming decades described.

Language: Английский

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