Molecular Biotechnology, Год журнала: 2024, Номер unknown
Опубликована: Сен. 13, 2024
Язык: Английский
Experimental & Molecular Medicine, Год журнала: 2024, Номер 56(4), С. 836 - 849
Опубликована: Апрель 1, 2024
Abstract Exosomes, which are nanosized vesicles secreted by cells, attracting increasing interest in the field of biomedical research due to their unique properties, including biocompatibility, cargo loading capacity, and deep tissue penetration. They serve as natural signaling agents intercellular communication, inherent ability carry proteins, lipids, nucleic acids endows them with remarkable therapeutic potential. Thus, exosomes can be exploited for diverse applications, chemotherapy, gene therapy, photothermal therapy. Moreover, capacity homotypic targeting self-recognition provides opportunities personalized medicine. Despite advantages novel agents, there several challenges optimizing efficiency structural stability defining exosome origins. Future should include development large-scale, quality-controllable production methods, refinement drug strategies, extensive vivo studies clinical trials. unresolved difficulties, use efficient, stable, safe delivery systems is an interesting area research. Therefore, this review describes summarizes cutting-edge published high-impact journals that have introduced or enhanced effects using a system past 2 years. We provide informative overview current state research, highlighting properties applications exosomes. also emphasize future directions, underscoring importance addressing key issues field. With review, we encourage researchers further develop exosome-based drugs application, such may among most promising next-generation therapeutics.
Язык: Английский
Процитировано
72
Advanced Composites and Hybrid Materials, Год журнала: 2024, Номер 7(6)
Опубликована: Окт. 23, 2024
Язык: Английский
Процитировано
20
Food & Function, Год журнала: 2023, Номер 14(23), С. 10401 - 10417
Опубликована: Янв. 1, 2023
Gut microbiota are closely related to lipopolysaccharide (LPS)-induced acute lung injury (ALI). Akkermansia muciniphila (A. muciniphila) maintains the intestinal barrier function and regulates balance of reduced glutathione/oxidized glutathione. However, it may be useful as a treatment strategy for LPS-induced injury. Our study aimed explore whether A. could improve by affecting gut microbiota. The administration effectively attenuated tissue damage significantly decreased oxidative stress inflammatory reaction induced LPS, with lower levels myeloperoxidase (MDA), enhanced superoxide dismutase (SOD) activity, pro-inflammatory cytokine levels, macrophage neutrophil infiltration. Moreover, maintained function, reshaped disordered microbial community, promoted secretion short-chain fatty acids (SCFAs). downregulated expression TLR2, MyD88 NF-kappa B (P < 0.05). Butyrate supplementation demonstrated significant improvement in response 0.05) mitigation histopathological mice ALI, thereby restoring butyric acid concentration. In conclusion, our findings indicate that inhibits accumulation cytokines attenuates activation TLR2/Myd88/NF-κB pathway due exerting anti-inflammatory effects through butyrate. This provides an experimental foundation potential application butyrate prevention ALI.
Язык: Английский
Процитировано
27
Trends in Pharmacological Sciences, Год журнала: 2024, Номер 45(4), С. 350 - 365
Опубликована: Март 19, 2024
Язык: Английский
Процитировано
11
Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)
Опубликована: Июнь 18, 2024
Abstract Acute lung injury ( ALI ) is a common complication in patients with severe burns and has complex pathogenesis high morbidity mortality rates. A variety of drugs have been identified the clinic for treatment ALI, but they toxic side effects caused by easy degradation body distribution throughout body. In recent years, as understanding mechanism underlying improved, scholars developed new nanomaterials that can be safely effectively targeted ALI. Most these methods involve such lipids, organic polymers, peptides, extracellular vesicles or cell membranes, inorganic nanoparticles other nanomaterials, which are to reach tissues perform their functions through active targeting passive targeting, process involves cells organelles. this review, first, mechanisms pathophysiological features occurrence after burn reviewed, potential therapeutic targets summarized, existing classified, possible problems challenges discussed provide reference development
Язык: Английский
Процитировано
10
Advanced Materials, Год журнала: 2024, Номер 36(21)
Опубликована: Апрель 8, 2024
Intrauterine adhesion (IUA) is characterized by the formation of fibrous scar tissue within uterine cavity, which significantly impacts female reproductive health and even leads to infertility. Unfortunately, severe cases IUA currently lack effective treatments. This study presents a novel approach that utilizes tumor necrosis factor-(TNF) stimulated gene 6 (TSG6)-modified exosomes (Exos) in conjunction with an injectable thermosensitive hydrogel (CS/GP) mitigate occurrence reducing endometrium fibrosis mouse model. demonstrate TSG6-modified Exos effectively inhibits activation inflammatory M1-like macrophages during initial stages inflammation maintains balance macrophage phenotypes (M1/M2) repair phase. Moreover, TSG6 interaction between endometrial stromal fibroblasts, thereby preventing fibroblasts into myofibroblasts. Furthermore, this research indicates CS/GP facilitates sustained release Exos, leading significant reduction both manifestations extent fibrosis. Collectively, through successful construction loaded progression achieved mitigating Consequently, holds promise for treatment IUA.
Язык: Английский
Процитировано
8
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Фев. 25, 2025
Extracellular vesicles (EVs) are critical mediators of intercellular communication, carrying bioactive cargo and displaying diverse surface components that reflect their cellular origins functions. The EV surface, composed proteins, lipids, glycocalyx elements, plays a pivotal role in targeting recipient cells, mediating biological interactions, enabling selective delivery. This review comprehensively examined the molecular architecture surfaces, linking biogenesis to functional diversity, highlights therapeutic diagnostic potential diseases such as cancer cardiovascular disorders. Additionally, we explore emerging applications EVs, including machine-learning-assisted analysis, chemical integration, cross-system combinations. also discusses some key challenges clinical translation EV-related technologies.
Язык: Английский
Процитировано
1
Biomaterials, Год журнала: 2024, Номер 308, С. 122562 - 122562
Опубликована: Апрель 1, 2024
Painful musculoskeletal disorders such as intervertebral disc (IVD) degeneration associated with chronic low back pain (termed "Discogenic pain", DBP), are a significant socio-economic burden worldwide and contribute to the growing opioid crisis. Yet there very few if any successful interventions that can restore tissue's structure function while also addressing symptomatic pain. Here we have developed novel non-viral gene therapy, using engineered extracellular vesicles (eEVs) deliver developmental transcription factor FOXF1 degenerated IVD in an vivo model. Injured IVDs treated eEVs loaded demonstrated robust sex-specific reductions behaviors compared control groups. Furthermore, restoration of animals were observed, increases height, tissue hydration, proteoglycan content, mechanical properties. This is first study successfully modulating animal model DBP eEV-based delivery genes. Such strategy be readily translated other painful disorders.
Язык: Английский
Процитировано
7
Frontiers in Immunology, Год журнала: 2023, Номер 14
Опубликована: Ноя. 23, 2023
Dental pulp stem cells (DPSCs) have received a lot of attention as regenerative medicine tool with strong immunomodulatory capabilities. The excessive inflammatory response involves variety immune cells, cytokines, and has considerable impact on tissue regeneration. use DPSCs for controlling inflammation the purpose treating inflammation-related diseases autoimmune disorders such supraspinal nerve inflammation, pulmonary airways, systemic lupus erythematosus, diabetes mellitus is likely to be safer more than traditional medicines. mechanism anti-inflammatory effects relatively complex, it may that they themselves or some substances secrete regulate through immune-related signaling pathways. Most current studies are still at laboratory cellular level animal model level, believed efforts researchers, DPSCs/SHED expected transformed into excellent drugs clinical treatment related diseases.
Язык: Английский
Процитировано
13
Advanced Science, Год журнала: 2023, Номер 11(6)
Опубликована: Дек. 7, 2023
Abstract Acute lung injury (ALI) is a severe respiratory disease with high mortality rate. The integrity of the pulmonary endothelial barrier influences development and prognosis ALI. Therefore, it has become an important target for ALI treatment. Extracellular vesicles (EVs) are promising nanotherapeutic agents against Herein, endothelium‐derived engineered extracellular (eEVs) that deliver microRNA‐125b‐5p (miRNA‐125b) to tissues exerting protective effect on reported. eEVs modified microvascular cell‐targeting peptides (LET) exhibit prolonged retention time in targeted cells vivo vitro. To improve efficacy EVs, miRNA‐125b loaded into EVs. Finally, LET‐EVs‐miRNA‐125b constructed. results show compared miRNA‐125b, EVs‐miRNA‐125b, most significant treatment Moreover, found have by inhibiting cell apoptosis, promoting angiogenesis, protecting intercellular junctions. Sequencing analysis reveals downregulates early growth response‐1 (EGR1) levels, which may be potential mechanism action. Taken together, these findings suggest can treat integrity.
Язык: Английский
Процитировано
13