Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Окт. 16, 2024
Bovine
mastitis
is
an
infectious
disease
that
causes
substantial
economic
losses
to
the
dairy
industry
worldwide.
Current
antibiotic
therapy
faces
issues
of
misuse
and
antimicrobial
resistance,
which
has
aroused
concerns
for
both
veterinary
human
medicine.
Thus,
this
study
explored
potential
Colo
EVs
(bovine
colostrum-derived
extracellular
vesicles)
address
mastitis.
Using
LPS-induced
murine
mammary
epithelial
cells
(HC11),
mouse
monocyte
macrophages
(RAW
264.7),
a
model
with
BALB/C
mice,
we
evaluated
safety
efficacy
EVs,
in
vivo
vitro.
had
favorable
biosafety
profiles,
promoting
cell
proliferation
migration
without
inducing
pathological
changes
after
injection
into
glands.
In
mastitis,
significantly
reduced
inflammation,
improved
inflammatory
scores,
preserved
tight
junction
proteins
while
protecting
milk
production.
Additionally,
vitro
experiments
demonstrated
downregulated
cytokine
expression,
markers,
attenuated
NF-κB
pathway
activation.
summary,
inferred
have
promise
as
therapeutic
approach
treatment,
owing
their
anti-inflammatory
properties,
potentially
mediated
through
signaling
modulation.
Abstract
Orthopedic
diseases,
such
as
osteoarthritis
and
fractures,
place
a
significant
burden
on
individuals
healthcare
systems
worldwide.
Extracellular
vesicles
(EVs),
which
are
membrane‐derived
particles,
have
emerged
novel
tool
in
the
field
of
orthopedics.
EVs
play
crucial
role
diagnosing,
regenerating,
treating
orthopedic
diseases.
In
terms
diagnosis,
serve
potential
biomarkers,
carrying
unique
donor
cell
information
circulating
effectively
bodily
fluids.
Specific
biomolecules
within
EVs,
including
proteins,
nucleic
acids,
microRNAs,
hold
promise
biological
markers
for
early
detection
monitoring
shown
promoting
bone
cartilage
regeneration.
They
can
enhance
tissue
regeneration
by
stimulating
various
stem
cells
to
proliferate,
migrate,
differentiate
into
mature
chondrocytes
osteocytes.
also
target
specific
tissues,
making
them
attractive
candidates
drug
delivery
efficiently
deliver
therapeutic
cargo,
anti‐inflammatory
agents
growth
factors,
affected
sites,
enhancing
treatment
efficacy
while
minimizing
toxicity
adverse
effects.
conclusion,
Extracellular
vesicle
(EV)-based
immunotherapeutics
have
emerged
as
promising
strategy
for
treating
diseases,
and
thus,
a
better
understanding
of
the
factors
that
regulate
EV
secretion
function
can
provide
insights
into
developing
advanced
therapies.
Here,
we
report
nutrient
availability,
even
changes
in
individual
components,
may
affect
biogenesis
composition
immune
cells
[e.g.,
macrophages
(Mφs)].
As
proof
concept,
EVs
from
M1-Mφ
under
glutamine-depleted
conditions
(EV
Molecular Therapy,
Год журнала:
2024,
Номер
32(9), С. 2950 - 2978
Опубликована: Июнь 22, 2024
Extracellular
vesicles
(EVs)
are
considered
a
vital
component
of
cell-to-cell
communication
and
represent
new
frontier
in
diagnostics
means
to
identify
pathways
for
therapeutic
intervention.
Recently,
studies
have
revealed
the
importance
tissue-derived
EVs
(Ti-EVs),
which
present
interstitial
spaces
between
cells,
as
they
better
underlying
physiology
complex,
multicellular
tissue
microenvironments
biology
disease.
native,
lipid
bilayer
membraned
nano-sized
particles
produced
by
all
cells
that
packaged
with
varied
functional
biomolecules
including
proteins,
lipids,
nucleic
acids.
They
implicated
short-
long-range
cellular
may
elicit
responses
recipient
cells.
To
date,
often
utilized
cultured
or
biological
fluids
source
do
not
capture
local
molecular
signatures
microenvironment.
Recent
work
utilizing
Ti-EVs
has
elucidated
novel
biomarkers
disease
provided
insights
into
mechanisms
lead
development
agents.
Still,
there
considerable
challenges
facing
current
studies.
This
review
explores
vast
potential
unique
Ti-EV
research
provides
considerations
future
seek
advance
this
exciting
field.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Авг. 30, 2024
Tissue-derived
extracellular
vesicles
(EVs)
are
emerging
as
pivotal
players
to
maintain
organ
homeostasis,
which
show
promise
a
next-generation
candidate
for
medical
use
with
extensive
source.
However,
the
detailed
function
and
therapeutic
potential
of
tissue
EVs
remain
insufficiently
studied.
Here,
through
bulk
single-cell
RNA
sequencing
analyses
combined
ultrastructural
examinations,
we
first
reveal
that
in
situ
liver
(LT-EVs)
contribute
intricate
regenerative
process
after
partial
hepatectomy
(PHx),
hepatocytes
primary
source
regenerating
liver.
Nanoscale
proteomic
profiling
further
identify
hepatocyte-specific
(Hep-EVs)
strengthened
release
carrying
proliferative
messages
PHx.
Moreover,
targeted
inhibition
Hep-EV
via
AAV-shRab27a
vivo
confirms
Hep-EVs
required
orchestrate
regeneration.
Mechanistically,
from
reciprocally
stimulate
hepatocyte
proliferation
by
promoting
cell
cycle
progression
Cyclin-dependent
kinase
1
(Cdk1)
activity.
Notably,
supplementing
demonstrates
translational
ameliorates
insufficient
This
study
provides
functional
mechanistic
framework
showing
governs
rapid
regeneration,
thereby
enriching
our
understanding
physiological
endogenous
regeneration
therapy.
Advanced Materials Interfaces,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 6, 2025
Abstract
Extracellular
vesicle
(EV)‐incorporated
hydrogels
have
emerged
as
promising
scaffolds
for
tissue
repair
due
to
their
ability
present
biological
cues.
However,
the
encapsulation
efficiency
and
distribution
of
EVs
within
still
require
improvement
enhance
healing
outcomes.
In
this
study,
a
novel
approach
is
developed
that
uses
crosslinkers
hydrogel
formation,
ensuring
are
at
every
crosslinking
point
thereby
achieving
both
functional
incorporation
uniform
EVs.
Amphiphilic
molecules
with
various
groups
successfully
inserted
into
EV
membrane,
enabling
macromers,
which
versatile
multiple
chemistries.
EV‐crosslinked
exhibited
faster
stress
relaxation
properties
stretchability
compared
crosslinked
traditional
elastic
polymers,
promoting
enhanced
cell
spreading
proliferation.
Additionally,
it
demonstrated
could
proteins
throughout
network
while
maintaining
activity.
Using
VEGF‐loaded
crosslinkers,
induced
endothelial
clustering
sprouting
successfully,
indicating
early
angiogenic
responses.
These
results
underscore
potential
engineering
regenerative
medicine,
offering
tunable
mechanical
capacity
effective
protein
delivery.
Frontiers in Veterinary Science,
Год журнала:
2024,
Номер
11
Опубликована: Янв. 25, 2024
Extracellular
vesicles
are
nanoscale
that
transport
signals
between
cells,
mediating
both
physiological
and
pathological
processes.
EVs
facilitate
conserved
intercellular
communication.
By
transferring
bioactive
molecules
coordinate
systemic
responses,
regulating
homeostasis,
immunity,
disease
progression.
Given
their
biological
importance
involvement
in
pathogenesis,
show
promise
as
biomarkers
for
veterinary
diagnosis,
candidates
vaccine
production,
treatment
agents.
Additionally,
different
or
engineering
methods
could
be
used
to
boost
the
capability
of
extracellular
vesicles.
Despite
emerging
interest,
EV
research
has
been
predominantly
human-based.
Critical
knowledge
gaps
remain
regarding
isolation
protocols,
cargo
loading
mechanisms,
vivo
biodistribution,
species-specific
functions.
Standardized
characterization
validation
lacking.
Regulatory
uncertainties
impede
clinical
translation.
Advances
fundamental
biology
technology
needed
propel
field
forward.
This
review
introduces
from
a
perspective
by
introducing
latest
studies,
highlighting
potential
while
analyzing
challenges
motivate
expanded
investigation
Aging and Disease,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Although
significant
progress
has
been
made
in
early
lung
cancer
screening
over
the
past
decade,
it
remains
one
of
most
prevalent
and
deadliest
forms
worldwide.
Exosomal
proteomics
emerged
as
a
transformative
field
research,
with
potential
to
redefine
diagnostics,
prognostic
assessments,
therapeutic
strategies
through
lens
precision
medicine.
This
review
discusses
recent
advances
exosome-related
proteomic
glycoproteomic
technologies,
highlighting
their
revolutionise
treatment
by
addressing
issues
heterogeneity,
integrating
multiomics
data,
utilising
advanced
analytical
methods.
While
these
technologies
show
promise,
there
are
obstacles
overcome
before
they
can
be
widely
implemented,
such
need
for
standardization,
gaps
clinical
application,
importance
dynamic
monitoring.
Future
directions
should
aim
challenges
fully
utilize
exosomal
cancer.
promises
new
era
personalized
medicine
that
leverages
molecular
complexity
exosomes
groundbreaking
advancements
detection,
prognosis,
treatment.
Advanced Materials,
Год журнала:
2023,
Номер
36(37)
Опубликована: Дек. 2, 2023
Abstract
Extracellular
vesicles
(EVs)
are
cell‐secreted
biological
nanoparticles
that
critical
mediators
of
intercellular
communication.
They
contain
diverse
bioactive
components,
which
promising
diagnostic
biomarkers
and
therapeutic
agents.
Their
nanosized
membrane‐bound
structures
innate
ability
to
transport
functional
cargo
across
major
barriers
make
them
candidates
as
drug
delivery
vehicles.
However,
the
complex
biology
heterogeneity
EVs
pose
significant
challenges
for
their
controlled
actionable
applications
in
diagnostics
therapeutics.
Recently,
DNA
molecules
with
high
biocompatibility
emerge
excellent
blocks
surface
engineering
EVs.
The
robust
Watson–Crick
base
pairing
resulting
programmable
nanomaterials
provide
EV
precise
structural
customization
adjustable
physical
chemical
properties,
creating
unprecedented
opportunities
biomedical
applications.
This
review
focuses
on
recent
advances
utilization
engineer
surfaces.
biology,
function,
summarized
state‐of‐the‐art
achievements
isolation,
analysis,
based
introduced.
Finally,
new
frontiers
discussed.
