Advanced Materials,
Год журнала:
2024,
Номер
36(33)
Опубликована: Июнь 14, 2024
Computed
Axial
Lithography
(CAL)
is
an
emerging
technology
for
manufacturing
complex
parts,
all
at
once,
by
circumventing
the
traditional
layered
approach
using
tomography.
Overprinting,
a
unique
additive
capability
of
CAL,
allows
3D
geometry
to
be
formed
around
prepositioned
insert
where
occlusion
light
compensated
other
angular
projections.
This
method
opens
door
novel
applications
within
multi-material
systems
such
as
endoskeletal
robots.
Herein,
this
work
presents
one
application
with
simple
Gelatin
Methacrylate
(GelMA)hydrogel
osmotic
actuator
embedded
system.
GelMA
ideal
material
it
swellable
and
has
reversible
thermal
gelation,
enabling
suspension
endoskeleton
during
printing.
By
tuning
formulation,
design,
post-processing,
swelling-induced
bending
actuation
60
degrees
achieved.
To
aid
in
printing
process,
computational
determining
absolute
dose
absorbed
resin
allowing
print
time
prediction
also
proposed.
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
13(16)
Опубликована: Март 30, 2024
Abstract
In
the
last
30
years,
there
are
≈60
000
publications
about
electrospun
nanofibers,
but
it
is
still
unclear
whether
nanoscale
fibers
really
necessary
for
tissue
engineering
scaffolds.
The
present
report
puts
forward
this
argument
and
reveals
that
compared
with
microfibers
diameter
of
≈3
µm
(named
as
“oligo‐micro
fiber”)
more
appropriate
scaffolds
owing
to
their
better
cell
infiltration
ability
caused
by
larger
pores
available
nuclear
deformation.
To
further
increase
pore
sizes,
poly(ε‐caprolactone)
(PCL)
fabricated
using
latticed
collectors
meshes.
Fiber
orientation
leads
sufficient
mechanical
strength
albeit
increases
porosity.
exhibit
good
biocompatibility
improve
infiltration.
Under
aortic
conditions
in
vitro,
performances
satisfactory
terms
acute
systolic
hemodynamic
functionality,
except
higher
regurgitation
fraction
enlarged
pores.
This
hierarchical
scaffold
sparse
macropores
oligo‐micro
filaments
provides
new
insights
into
design
scaffolds,
may
provide
living
heart
valves
regenerative
capabilities
patients
severe
valve
disease
future.
Chemical Reviews,
Год журнала:
2024,
Номер
124(14), С. 8787 - 8822
Опубликована: Июль 5, 2024
Harnessing
light
for
cross-linking
of
photoresponsive
materials
has
revolutionized
the
field
3D
printing.
A
wide
variety
techniques
leveraging
broad-spectrum
shaping
have
been
introduced
as
a
way
to
achieve
fast
and
high-resolution
printing,
with
applications
ranging
from
simple
prototypes
biomimetic
engineered
tissues
regenerative
medicine.
Conventional
light-based
printing
use
material
in
layer-by-layer
fashion
produce
complex
parts.
Only
recently,
new
emerged
which
deploy
multidirection,
tomographic,
light-sheet
or
filamented
image
projections
deep
into
volume
resin-filled
vat
photoinitiation
cross-linking.
These
Deep
Vat
(DVP)
approaches
alleviate
need
layer-wise
enable
unprecedented
fabrication
speeds
(within
few
seconds)
high
resolution
(>10
μm).
Here,
we
elucidate
physics
chemistry
these
processes,
their
commonalities
differences,
well
emerging
biomedical
non-biomedical
fields.
Importantly,
highlight
limitations,
future
scope
research
that
will
improve
scalability
applicability
DVP
engineering
medicine
applications.
Organ
damage
or
failure
arising
from
injury,
disease,
and
aging
poses
challenges
due
to
the
body's
limited
regenerative
capabilities.
transplantation
presents
issues
of
donor
shortages
immune
rejection
risks,
necessitating
innovative
solutions.
The
3D
bioprinting
organs
on
demand
offers
promise
in
tissue
engineering
medicine.
In
this
review,
we
explore
state-of-the-art
technologies,
with
a
focus
bioink
cell
type
selections.
We
follow
discussions
advances
solid
organs,
such
as
heart,
liver,
kidney,
pancreas,
highlighting
importance
vascularization
integration.
Finally,
provide
insights
into
key
future
directions
context
clinical
translation
bioprinted
their
large-scale
production.
Biomaterials Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Advancement
of
vascular
models
from
simple
2D
culture
to
complex
vessel-on-a-chip
platforms
through
integration
microfluidics,
biomimetic
hydrogels,
and
3D
bioprinting,
enabling
controlled
investigation
thrombosis
mechanisms.
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 5, 2025
Engineered
living
systems
(ELSs)
represent
purpose-driven
assemblies
of
components,
encompassing
cells,
biomaterials,
and
active
agents,
intricately
designed
to
fulfill
diverse
biomedical
applications.
Gelatin
its
derivatives
have
been
used
extensively
in
ELSs
owing
their
mature
translational
pathways,
favorable
biological
properties,
adjustable
physicochemical
characteristics.
This
review
explores
the
intersection
gelatin
with
fabrication
techniques,
offering
a
comprehensive
examination
synergistic
potential
creating
for
various
applications
biomedicine.
It
offers
deep
dive
into
gelatin,
including
structures
production,
sources,
processing,
properties.
Additionally,
techniques
employing
derivatives,
generic
microfluidics,
3D
printing
methods.
Furthermore,
it
discusses
based
on
regenerative
engineering
as
well
cell
therapies,
bioadhesives,
biorobots,
biosensors.
Future
directions
challenges
are
also
examined,
highlighting
emerging
trends
areas
improvements
innovations.
In
summary,
this
underscores
significance
gelatin-based
advancing
lays
groundwork
guiding
future
research
developments
within
field.
Abstract
3D
printing
has
greatly
improved
the
precision
of
cell
and
biomaterial
placement,
enabling
accurate
reproduction
tissue
models
with
sustainable
potential.
Various
techniques,
including
inkjet
printing,
extrusion‐based
vat
photopolymerization,
offer
unique
advantages
but
often
fail
to
replicate
full
complexity
native
tissues
because
material
scalability
limitations.
Hybrid
bioprinting,
combining
multiple
techniques
in
a
single
process,
shown
great
potential
creating
complex
multifunctional
capabilities,
ranging
from
patient‐specific
implant
fabrication
full‐scale
organ
development.
It
capitalizes
on
strengths
integration
sustainable,
renewable
biomaterials
at
varying
resolutions,
nano
microscale.
This
approach
addresses
both
biological
environmental
responsibility
by
minimizing
waste
enhancing
sustainability
engineering
processes.
Despite
progress,
substantial
gap
remains
between
current
technologies
bioengineering
requirements.
A
deep
understanding
hybrid
its
underlying
mechanisms
is
crucial.
Herein,
this
review
summarizes
discusses
recent
advancements
systems
for
fabricating
multiscale
hierarchical
models,
focusing
challenges
field.
aims
insights
identify
key
requirements
advancing
technology
toward
developing
functional,
biomimetic
constructs.
The
field
of
biomedical
design
and
manufacturing
has
been
rapidly
evolving,
with
implants
grafts
featuring
complex
3D
constraints
materials
distributions.
By
combining
a
new
coding-based
modeling
approach
high-throughput
volumetric
printing,
is
demonstrated
to
transform
the
way
shapes
are
designed
fabricated
for
applications.
Here,
an
algorithmic
voxel-based
used
that
can
generate
large
library
porous
structures,
auxetic
meshes
cylinders,
or
perfusable
constructs.
deploying
finite
cell
within
framework,
arrays
selected
designs
be
computationally
modeled.
Finally,
schemes
in
conjunction
approaches
multi-material
printing
based
on
thiol-ene
photoclick
chemistry
fabricate
heterogeneous
shapes.
Collectively,
design,
fabrication
techniques
toward
wide
spectrum
products
such
as
actuators,
grafts,
tissue
disease
models.
Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 2, 2024
Abstract
The
effective
replication
of
microtubular
structures
in
tissue
engineering
remains
a
great
challenge.
In
this
study,
the
temperature‐responsive
characteristics
poly(
N
‐isopropylacrylamide)
(pNIPAM)
to
create
intricate,
high‐resolution
tubular
through
shrinking
mechanism
is
investigated
by
exploring
2
thermosensitive
hydrogels–gelatin
methacryloyl
(gelMA)
and
silk
fibroin
(silkMA)–combined
with
pNIPAM.
Systematic
investigations
revealed
precise
control
behavior
at
elevated
temperatures
(33–37
°C)
as
function
polymer
concentration.
hydrogel
sizes
reduce
≈15%
from
room
temperature
(RT)
33
°C
≈40%
RT
37
for
both
types.
affects
mechanical
properties,
increasing
compressive
modulus
≈2.8‐fold
gelMA‐pNIPAM
gels
≈5.1‐fold
silkMA‐pNIPAM
°C.
Combined
volumetric
printing,
these
materials
achieve
resolution
enhancements
≈20%
positive
features
≈70%
negative
features,
enabling
creation
complex,
within
seconds,
open
channels
(≈50
µm).
GelMA‐pNIPAM
hydrogels
show
better
cell
compatibility
compared
hydrogels,
promoting
adhesion
viability.
This
study
demonstrates
hydrogels'
capability
engineer
printing–an
efficient
route
fabricate
microenvironments
mimicking
native
tissues
potential
developing
relevant
vitro
models.
