bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 16, 2024
ABSTRACT
Engineered
living
materials
(ELMs)
made
of
bacteria
in
hydrogels
have
shown
considerable
promise
for
therapeutic
applications
since
they
offer
the
possibility
to
achieve
controlled
and
prolonged
release
complex
biopharmaceuticals
at
low
costs
with
reduced
wastage.
While
most
ELMs
use
E.
coli
as
component
due
its
large
genetic
toolbox,
live
biotherapeutic
clinical
trials
are
lactic
acid
native
health
benefits
offer.
Among
these,
lactobacilli
largest
family
probiotic
that
being
investigated
their
potential
almost
all
sites
body
host
a
microbiome.
A
major
factor
limiting
is
limited
toolbox.
In
this
study,
we
build
upon
our
recent
work
expand
programmability
lactobacillus
strain
(
Lactiplantibacillus
plantarum
WCFS1)
protein
secretion
integrate
it
into
simple,
cost-effective,
biocompatible
alginate
bead
encapsulation
format
develop
an
ELM.
We
demonstrate
recombinant
up
14
days
from
ELM,
thereby
terming
PEARL
-
Protein
Eluting
Alginate
Recombinant
Lactobacilli.
Notably,
offered
multiple
such
preventing
bacterial
outgrowth,
stabilizing
profiles
over
time,
cytotoxicity
caused
by
metabolites.
These
findings
mutual
combining
proteins
biomedical
applications.
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 19, 2025
Abstract
Friction‐induced
energy
consumption
is
a
significant
global
concern,
driving
researchers
to
explore
advanced
lubrication
materials.
In
nature,
vital
for
the
life
cycle
of
animals,
plants,
and
humans,
playing
key
roles
in
movement,
predation,
decomposition.
After
billions
years
evolution,
natural
exhibits
remarkable
professionalism,
high
efficiency,
durability,
intelligence,
offering
valuable
insights
designing
This
review
focuses
on
mechanisms
organisms
advancements
biomimetic
soft
matter
It
begins
by
summarizing
common
biological
behaviors
their
underlying
mechanisms,
followed
current
design
strategies
The
then
outlines
development
performance
these
materials
based
different
strategies.
Finally,
it
discusses
potential
research
directions
prospects
will
be
resource
advancing
Abstract
Engineered
living
materials
(ELMs)
made
of
bacteria
in
hydrogels
have
shown
considerable
promise
for
therapeutic
applications
through
controlled
and
sustained
release
complex
biopharmaceuticals
at
low
costs
with
reduced
wastage.
While
most
ELMs
use
E.
coli
due
to
its
large
genetic
toolbox,
live
biotherapeutic
development
are
lactic
acid
native
health
benefits
they
offer.
Among
these,
lactobacilli
form
the
largest
family
probiotics
potential
almost
all
sites
body
a
microbiome.
A
major
factor
limiting
is
their
limited
toolbox.
This
study
expands
on
recent
work
expand
programmability
probiotic
Lactiplantibacillus
plantarum
WCFS1
protein
secretion
encapsulate
it
simple,
cost‐effective,
biocompatible
core–shell
alginate
bead
develop
an
ELM.
The
recombinant
proteins
demonstrated,
even
up
14
days
from
this
ELM,
thereby
terming
PEARL
–
Protein
Eluting
Alginate
Recombinant
Lactobacilli.
Notably,
lactobacillus
encapsulation
offered
like
bacterial
containment,
profile
stabilization,
metabolite‐induced
cytotoxicity
prevention.
These
findings
demonstrate
mutual
combining
proteins.
Engineered
Living
Materials
(ELMs)
combine
synthetic
biology
with
artificial
materials
to
create
biohybrid
living
systems
capable
of
replicating,
self-repairing,
and
responding
external
stimuli.
Due
their
self-optimization
abilities,
these
hold
great
potential
for
biotechnological
applications.
This
study
is
a
first
step
toward
ELMs
based
on
DNA
hydrogels,
focusing
the
production
using
exoelectrogenic
bacterium
Shewanella
oneidensis.
To
equip
functionality
needed
building
inducible
cell
surface
anchors
are
developed,
which
can
bind
exogenous
polymerase
via
SpyCatcher/SpyTag
(SC/ST)
technology.
The
process
parameters
in
situ
hydrogels
established,
enabling
development
context
bacteria
time.
Using
an
extracellular
nuclease-deficient
S.
oneidensis
strain,
stable
biofilms
generated
directly
bioelectrochemical
systems,
showing
current
generation.
Given
high
programmability
functionalization
it
believed
that
this
represents
significant
establishing
dynamic
material
exhibit
both
conductivity
metabolic
activity.
Trends in biotechnology,
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 1, 2024
Recent
advances
in
engineered
bacterial
therapeutics
underscore
their
potential
treating
diseases
via
targeted,
live
interventions.
Despite
promising
performance
early
clinical
phases,
no
therapeutic
bacteria
have
yet
received
approval,
primarily
due
to
challenges
proving
efficacy
while
ensuring
biosafety.
Material
science
innovations,
particularly
the
encapsulation
of
within
hydrogels,
present
a
avenue
enhance
survival,
efficacy,
and
safety
applications.
This
review
discusses
this
interdisciplinary
approach
develop
living
materials.
Hydrogels
not
only
safeguard
from
harsh
physiological
conditions
but
also
enable
controlled
release
prevent
unintended
dissemination.
The
strategic
use
materials
could
redefine
delivery
functionality
therapeutics,
facilitating
translation.
Materials,
Год журнала:
2025,
Номер
18(7), С. 1445 - 1445
Опубликована: Март 25, 2025
A
meta-analysis
was
conducted
to
assess
the
evolution
of,
applications
and
recent
advancements
in
materials
surface
treatments
for
contact
lenses.
This
study
aimed
comprehensively
synthesize
available
data,
focusing
on
innovations
that
enhance
vision
correction,
comfort,
safety
while
emphasizing
sustainability
as
a
critical
factor
future
development.
Registered
with
PROSPERO,
this
analysis
adhered
PRISMA
AMSTAR-2
guidelines.
systematic
review
of
databases
including
PubMed,
Web
Science,
Scopus
performed
studies
published
between
2019
2024,
without
language
restrictions.
Observational
optical
lens
were
included,
random-effects
model
used
address
high
heterogeneity
among
included
studies.
From
nine
analyzed,
significant
identified
regarding
functional
properties
treatments.
Key
technologies
like
self-lubricating
lenses
reduce
friction,
nanogels
prolonged
therapeutic
drug
delivery,
coatings
minimize
protein
lipid
deposition,
ensuring
greater
comfort
extended
wearability.
Additionally,
biodegradable
eco-friendly
underscore
industry’s
commitment
reducing
environmental
impact
lenses,
addressing
challenges
related
disposal
recycling.
These
highlight
potential
integrating
improvements
sustainability,
paving
way
more
effective
environmentally
responsible
Materials Horizons,
Год журнала:
2024,
Номер
11(22), С. 5768 - 5776
Опубликована: Янв. 1, 2024
A
successful
flexible
wearable
not
only
has
to
fulfill
its
function,
but
also
ensure
long-term
wettability
and
comfort
during
wearing.
In
biological
systems,
tears
spread
rapidly
across
the
cornea
clear
imaging
while
slowly
evaporating
maintain
moisture
in
eyes.
This
dynamic
behavior
of
'rapid
spread,
slow
evaporation'
ensures
durative
humidity
comfort,
which
can
provide
design
guidelines
for
continuous
devices.
However,
realizing
this
process
vitro
remains
a
challenge.
Herein,
inspired
by
healthy
ocular
surface,
we
biomimetically
construct
hybrid
surface
featuring
mucin-like
hydrophilic
layer@hydrogel
nanowire
arrays
(HL@HNWs).
droplet
(2
μL)
spreads
into
thin
film,
stabilizing
∼10
minutes,
whereas
contrast
sample
ruptures
dewets
within
1
minute.
We
demonstrate
that
enhancing
proportion
hydrated
water
(HW),
includes
intermediate
(IW)
bound
(BW),
introducing
capillary
resistance
could
synergistically
stabilize
film
improve
wettability.
Hydrogel-based
array
contact
lenses
wear,
stable
substantially
superior
visual
quality.
