PEARL: Protein Eluting Alginate with Recombinant Lactobacilli DOI

Varun Sai Tadimarri,

Marc Blanch-Asensio,

Ketaki Deshpande

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 16, 2024

ABSTRACT Engineered living materials (ELMs) made of bacteria in hydrogels have shown considerable promise for therapeutic applications since they offer the possibility to achieve controlled and prolonged release complex biopharmaceuticals at low costs with reduced wastage. While most ELMs use E. coli as component due its large genetic toolbox, live biotherapeutic clinical trials are lactic acid native health benefits offer. Among these, lactobacilli largest family probiotic that being investigated their potential almost all sites body host a microbiome. A major factor limiting is limited toolbox. In this study, we build upon our recent work expand programmability lactobacillus strain ( Lactiplantibacillus plantarum WCFS1) protein secretion integrate it into simple, cost-effective, biocompatible alginate bead encapsulation format develop an ELM. We demonstrate recombinant up 14 days from ELM, thereby terming PEARL - Protein Eluting Alginate Recombinant Lactobacilli. Notably, offered multiple such preventing bacterial outgrowth, stabilizing profiles over time, cytotoxicity caused by metabolites. These findings mutual combining proteins biomedical applications.

Language: Английский

Tribology in Nature: Inspirations for Advanced Lubrication Materials DOI Open Access
Chenxi Qin, Hao Yang, Yaqiong Lü

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Abstract Friction‐induced energy consumption is a significant global concern, driving researchers to explore advanced lubrication materials. In nature, vital for the life cycle of animals, plants, and humans, playing key roles in movement, predation, decomposition. After billions years evolution, natural exhibits remarkable professionalism, high efficiency, durability, intelligence, offering valuable insights designing This review focuses on mechanisms organisms advancements biomimetic soft matter It begins by summarizing common biological behaviors their underlying mechanisms, followed current design strategies The then outlines development performance these materials based different strategies. Finally, it discusses potential research directions prospects will be resource advancing

Language: Английский

Citations

3
A practical workflow for cytocompatibility assessment of living therapeutic materials DOI Creative Commons

Joëlle Mekontso,

Usama Farrukh,

Sara Trujillo

et al.

Biomaterials Advances, Journal Year: 2025, Volume and Issue: 169, P. 214182 - 214182

Published: Jan. 10, 2025

Language: Английский

Citations

1
Engineered bacterial therapeutics with material solutions DOI Creative Commons
Sourik Dey, Shrikrishnan Sankaran

Trends in biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: July 1, 2024

Recent advances in engineered bacterial therapeutics underscore their potential treating diseases via targeted, live interventions. Despite promising performance early clinical phases, no therapeutic bacteria have yet received approval, primarily due to challenges proving efficacy while ensuring biosafety. Material science innovations, particularly the encapsulation of within hydrogels, present a avenue enhance survival, efficacy, and safety applications. This review discusses this interdisciplinary approach develop living materials. Hydrogels not only safeguard from harsh physiological conditions but also enable controlled release prevent unintended dissemination. The strategic use materials could redefine delivery functionality therapeutics, facilitating translation.

Language: Английский

Citations

5
PEARL: Protein Eluting Alginate with Recombinant Lactobacilli DOI Creative Commons

Varun Sai Tadimarri,

Marc Blanch‐Asensio,

Ketaki Deshpande

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Abstract Engineered living materials (ELMs) made of bacteria in hydrogels have shown considerable promise for therapeutic applications through controlled and sustained release complex biopharmaceuticals at low costs with reduced wastage. While most ELMs use E. coli due to its large genetic toolbox, live biotherapeutic development are lactic acid native health benefits they offer. Among these, lactobacilli form the largest family probiotics potential almost all sites body a microbiome. A major factor limiting is their limited toolbox. This study expands on recent work expand programmability probiotic Lactiplantibacillus plantarum WCFS1 protein secretion encapsulate it simple, cost‐effective, biocompatible core–shell alginate bead develop an ELM. The recombinant proteins demonstrated, even up 14 days from this ELM, thereby terming PEARL – Protein Eluting Alginate Recombinant Lactobacilli. Notably, lactobacillus encapsulation offered like bacterial containment, profile stabilization, metabolite‐induced cytotoxicity prevention. These findings demonstrate mutual combining proteins.

Language: Английский

Citations

0
Engineered microbial living matter for diagnostics, prevention, and therapy DOI Creative Commons

Ali Khazem,

Rosanne Schmachtenberg,

Anke Weiand

et al.

Current Opinion in Biotechnology, Journal Year: 2025, Volume and Issue: 92, P. 103269 - 103269

Published: Feb. 6, 2025

Language: Английский

Citations

0
A spatial confinement biological heterogeneous cascade nanozyme composite hydrogel combined with nitric oxide gas therapy for enhanced treatment of psoriasis and diabetic wound DOI
Yuying Zhang, Cunpeng Nie, Zishu Wang

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160629 - 160629

Published: Feb. 1, 2025

Language: Английский

Citations

0
Biofilm-Mediated Infections; Novel Therapeutic Approaches and Harnessing Artificial Intelligence for Early Detection and Treatment of Biofilm-Associated Infections DOI

Muhammad Bilal Habib,

Ghanwa Batool,

Naseer Ali Shah

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107497 - 107497

Published: March 1, 2025

Language: Английский

Citations

0
Meta-Analysis of Materials and Treatments Used in Contact Lenses: Implications for Lens Characteristics DOI Open Access
Ana Paula Oliveira, Clara Martínez-Pérez

Materials, Journal Year: 2025, Volume and Issue: 18(7), P. 1445 - 1445

Published: March 25, 2025

A meta-analysis was conducted to assess the evolution of, applications and recent advancements in materials surface treatments for contact lenses. This study aimed comprehensively synthesize available data, focusing on innovations that enhance vision correction, comfort, safety while emphasizing sustainability as a critical factor future development. Registered with PROSPERO, this analysis adhered PRISMA AMSTAR-2 guidelines. systematic review of databases including PubMed, Web Science, Scopus performed studies published between 2019 2024, without language restrictions. Observational optical lens were included, random-effects model used address high heterogeneity among included studies. From nine analyzed, significant identified regarding functional properties treatments. Key technologies like self-lubricating lenses reduce friction, nanogels prolonged therapeutic drug delivery, coatings minimize protein lipid deposition, ensuring greater comfort extended wearability. Additionally, biodegradable eco-friendly underscore industry’s commitment reducing environmental impact lenses, addressing challenges related disposal recycling. These highlight potential integrating improvements sustainability, paving way more effective environmentally responsible

Language: Английский

Citations

0
Extracellular Bacterial Production of DNA Hydrogels–Toward Engineered Living Materials DOI Creative Commons

Philipp Gaspers,

Christoph Bickmann,

C. Wallner

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Engineered Living Materials (ELMs) combine synthetic biology with artificial materials to create biohybrid living systems capable of replicating, self-repairing, and responding external stimuli. Due their self-optimization abilities, these hold great potential for biotechnological applications. This study is a first step toward ELMs based on DNA hydrogels, focusing the production using exoelectrogenic bacterium Shewanella oneidensis. To equip functionality needed building inducible cell surface anchors are developed, which can bind exogenous polymerase via SpyCatcher/SpyTag (SC/ST) technology. The process parameters in situ hydrogels established, enabling development context bacteria time. Using an extracellular nuclease-deficient S. oneidensis strain, stable biofilms generated directly bioelectrochemical systems, showing current generation. Given high programmability functionalization it believed that this represents significant establishing dynamic material exhibit both conductivity metabolic activity.

Language: Английский

Citations

0
Rapid spread, slow evaporation: a long-lasting water film on hydrogel nanowire arrays for continuous wearables DOI Creative Commons

Peijia Li,

Yilin Wang,

Ming Qiu

et al.

Materials Horizons, Journal Year: 2024, Volume and Issue: 11(22), P. 5768 - 5776

Published: Jan. 1, 2024

A successful flexible wearable not only has to fulfill its function, but also ensure long-term wettability and comfort during wearing. In biological systems, tears spread rapidly across the cornea clear imaging while slowly evaporating maintain moisture in eyes. This dynamic behavior of 'rapid spread, slow evaporation' ensures durative humidity comfort, which can provide design guidelines for continuous devices. However, realizing this process vitro remains a challenge. Herein, inspired by healthy ocular surface, we biomimetically construct hybrid surface featuring mucin-like hydrophilic layer@hydrogel nanowire arrays (HL@HNWs). droplet (2 μL) spreads into thin film, stabilizing ∼10 minutes, whereas contrast sample ruptures dewets within 1 minute. We demonstrate that enhancing proportion hydrated water (HW), includes intermediate (IW) bound (BW), introducing capillary resistance could synergistically stabilize film improve wettability. Hydrogel-based array contact lenses wear, stable substantially superior visual quality.

Language: Английский

Citations

2