National Science Review,
Год журнала:
2024,
Номер
11(4)
Опубликована: Янв. 25, 2024
Tissue
regeneration
is
a
complicated
process
that
relies
on
the
coordinated
effort
of
nervous,
vascular
and
immune
systems.
While
nervous
system
plays
crucial
role
in
tissue
regeneration,
current
engineering
approaches
mainly
focus
restoring
function
injury-related
cells,
neglecting
guidance
provided
by
nerves.
This
has
led
to
unsatisfactory
therapeutic
outcomes.
Herein,
we
propose
new
generation
engineered
neural
constructs
from
perspective
induction,
which
offers
versatile
platform
for
promoting
multiple
regeneration.
Specifically,
consist
inorganic
biomaterials
stem
cells
(NSCs),
where
endows
NSCs
with
enhanced
biological
activities
including
proliferation
differentiation.
Through
animal
experiments,
show
effectiveness
repairing
central
injuries
recovery.
More
importantly,
also
stimulate
osteogenesis,
angiogenesis
neuromuscular
junction
formation,
thus
bone
skeletal
muscle,
exhibiting
its
performance.
These
findings
suggest
inorganic-biomaterial/NSC-based
represents
promising
avenue
inducing
recovery
varying
tissues
organs.
Abstract
For
the
past
two
decades,
function
of
intrabony
nerves
on
bone
has
been
a
subject
intense
research,
while
is
still
hidden
in
corner.
In
present
review,
possible
crosstalk
between
and
peripheral
will
be
comprehensively
analyzed.
Peripheral
participate
development
repair
via
host
signals
generated
through
secretion
neurotransmitters,
neuropeptides,
axon
guidance
factors
neurotrophins,
with
additional
contribution
from
nerve‐resident
cells.
return,
contributes
to
this
microenvironmental
rendezvous
by
housing
within
its
internal
milieu
provide
mechanical
support
protective
shelf.
A
large
ensemble
chemical,
mechanical,
electrical
cues
works
harmony
marrow
stromal
cells
regulation
nerves.
The
not
limited
physiological
state,
but
also
involved
various
diseases
including
osteoporosis,
osteoarthritis,
heterotopic
ossification,
psychological
stress‐related
abnormalities,
related
tumors.
This
may
harnessed
design
tissue
engineering
scaffolds
for
defects
or
targeted
treatment
Cellular & Molecular Biology Letters,
Год журнала:
2022,
Номер
27(1)
Опубликована: Сен. 4, 2022
Abstract
Osteoporotic
fractures
lead
to
increased
disability
and
mortality
in
the
elderly
population.
With
rapid
increase
aging
population
around
globe,
more
effective
treatments
for
osteoporosis
osteoporotic
are
urgently
required.
The
underlying
molecular
mechanisms
of
believed
be
due
activity
osteoclasts,
decreased
osteoblasts,
or
both,
which
leads
an
imbalance
bone
remodeling
process
with
accelerated
resorption
attenuated
formation.
Currently,
available
clinical
have
mostly
focused
on
factors
influencing
remodeling;
however,
they
their
own
limitations
side
effects.
Recently,
cytokine
immunotherapy,
gene
therapy,
stem
cell
therapy
become
new
approaches
treatment
various
diseases.
This
article
reviews
latest
research
mechanisms,
as
well
how
this
underpins
current
potential
novel
osteoporosis.
Advanced Healthcare Materials,
Год журнала:
2022,
Номер
12(3)
Опубликована: Ноя. 3, 2022
Repairing
infected
bone
defects
is
a
challenge
in
the
field
of
orthopedics
because
limited
self-healing
capacity
tissue
and
susceptibility
refractory
materials
to
bacterial
activity.
Innervation
initiating
factor
for
regeneration
plays
key
regulatory
role
subsequent
vascularization,
ossification,
mineralization
processes.
Infection
leads
necrosis
local
nerve
fibers,
impeding
repair
defects.
Herein,
biomaterial
that
can
induce
skeletal-associated
neural
network
reconstruction
with
high
antibacterial
activity
proposed
treatment
A
photosensitive
conductive
hydrogel
prepared
by
incorporating
magnesium-modified
black
phosphorus
(BP@Mg)
into
gelatin
methacrylate
(GelMA).
The
near-infrared
irradiation-based
photothermal
photodynamic
endows
it
strong
activity,
improving
inflammatory
microenvironment
reducing
bacteria-induced
damage.
nanosheets
bioactive
ions
released
from
BP@Mg
synergistically
improve
migration
secretion
Schwann
cells,
promote
neurite
outgrowth,
facilitate
innerved
regeneration.
In
an
skull
defect
model,
GelMA-BP@Mg
shows
efficient
promotes
CGRP+
fiber
phototherapy
provides
novel
strategy
based
on
innervation
repair.
Experimental & Molecular Medicine,
Год журнала:
2022,
Номер
54(11), С. 1844 - 1849
Опубликована: Ноя. 29, 2022
Abstract
The
mammalian
skeletal
system
is
densely
innervated
by
both
neural
and
vascular
networks.
Peripheral
nerves
in
the
skeleton
include
sensory
sympathetic
nerves.
crosstalk
between
tissues
critical
for
development
regeneration.
cellular
processes
of
osteogenesis
angiogenesis
are
coupled
physiological
pathophysiological
contexts.
molecular
regulation
have
yet
to
be
fully
defined.
This
review
will
provide
a
detailed
characterization
regulatory
role
blood
vessels
during
bone
Furthermore,
given
importance
spatial
relationship
bone,
we
discuss
neurovascular
coupling
pathological
formation.
A
better
understanding
interactions
inform
future
novel
therapeutic
targeting
clinical
repair
Advanced Materials,
Год журнала:
2023,
Номер
35(42)
Опубликована: Июль 12, 2023
Neural-vascular
networks
are
densely
distributed
through
periosteum,
cortical
bone,
and
cancellous
which
is
of
great
significance
for
bone
regeneration
remodeling.
Although
significant
progress
has
been
made
in
tissue
engineering,
ineffective
regeneration,
delayed
osteointegration
still
remains
an
issue
due
to
the
ignorance
intrabony
nerves
blood
vessels.
Herein,
inspired
by
space-filling
polyhedra
with
open
architectures,
polyhedron-like
scaffolds
spatial
topologies
prepared
via
3D-printing
technology
mimic
meshwork
structure
bone.
Benefiting
from
its
topologies,
greatly
promoted
osteogenic
differentiation
mesenchymal
stem
cells
(BMSCs)
activating
PI3K-Akt
signals,
exhibiting
satisfactory
performance
on
angiogenesis
neurogenesis.
Computational
fluid
dynamic
(CFD)
simulation
elucidates
that
have
a
relatively
lower
area-weighted
average
static
pressure,
beneficial
osteogenesis.
Furthermore,
vivo
experiments
further
demonstrate
obviously
promote
formation
osteointegration,
as
well
inducing
vascularization
ingrowth
nerves,
leading
innervated
vascularized
regeneration.
Taken
together,
this
work
offers
promising
approach
fabricating
multifunctional
without
additional
exogenous
seeding
growth
factors,
holds
potential
functional
clinical
translation.
The
skeleton
is
a
highly
innervated
organ
in
which
nerve
fibers
interact
with
various
skeletal
cells.
Peripheral
endings
release
neurogenic
factors
and
sense
signals,
mediate
bone
metabolism
pain.
In
recent
years,
tissue
engineering
has
increasingly
focused
on
the
effects
of
nervous
system
regeneration.
Simultaneous
regeneration
nerves
through
use
materials
or
by
enhancement
endogenous
repair
signals
been
proven
to
promote
functional
Additionally,
emerging
information
mechanisms
interoception
central
regulation
homeostasis
provide
an
opportunity
for
advancing
biomaterials.
However,
comprehensive
reviews
this
topic
are
lacking.
Therefore,
review
provides
overview
relationship
between
regeneration,
focusing
applications.
We
discuss
novel
regulatory
explore
innovative
approaches
based
nerve-bone
interactions
Finally,
challenges
future
prospects
field
briefly
discussed.
ACS Nano,
Год журнала:
2024,
Номер
18(10), С. 7504 - 7520
Опубликована: Фев. 27, 2024
The
essential
role
of
the
neural
network
in
enhancing
bone
regeneration
has
often
been
overlooked
biomaterial
design,
leading
to
delayed
or
compromised
healing.
Engineered
mesenchymal
stem
cells
(MSCs)-derived
exosomes
are
becoming
increasingly
recognized
as
potent
cell-free
agents
for
manipulating
cellular
behavior
and
improving
therapeutic
effectiveness.
Herein,
MSCs
stimulated
with
nerve
growth
factor
(NGF)
regulate
exosomal
cargoes
improve
neuro-promotive
potential
facilitate
innervated
regeneration.
In
vitro
cell
experiments
showed
that
NGF-stimulated
MSCs-derived
(N-Exos)
obviously
improved
function
neurotrophic
effects
cells,
consequently,
osteogenic
osteo-reparative
cells.
Bioinformatic
analysis
by
miRNA
sequencing
pathway
enrichment
revealed
beneficial
N-Exos
may
partly
be
ascribed
NGF-elicited
multicomponent
miRNAs
subsequent
regulation
activation
MAPK
PI3K-Akt
signaling
pathways.
On
this
basis,
were
delivered
on
micropores
3D-printed
hierarchical
porous
scaffold
accomplish
sustained
release
profile
extended
bioavailability.
a
rat
model
distal
femoral
defect,
N-Exos-functionalized
significantly
induced
neurovascular
structure
formation
This
study
provided
feasible
strategy
modulate
functional
acquire
desirable
potential.
Furthermore,
developed
represent
promising
neurovascular-promotive
reparative
clinical
translation.
Military Medical Research,
Год журнала:
2024,
Номер
11(1)
Опубликована: Июнь 12, 2024
Abstract
In
addition
to
its
recognized
role
in
providing
structural
support,
bone
plays
a
crucial
maintaining
the
functionality
and
balance
of
various
organs
by
secreting
specific
cytokines
(also
known
as
osteokines).
This
reciprocal
influence
extends
these
modulating
homeostasis
development,
although
this
aspect
has
yet
be
systematically
reviewed.
review
aims
elucidate
bidirectional
crosstalk,
with
particular
focus
on
osteokines.
Additionally,
it
presents
unique
compilation
evidence
highlighting
critical
function
extracellular
vesicles
(EVs)
within
bone-organ
axes
for
first
time.
Moreover,
explores
implications
crosstalk
designing
implementing
bone-on-chips
assembloids,
underscoring
importance
comprehending
interactions
advancing
physiologically
relevant
vitro
models.
Consequently,
establishes
robust
theoretical
foundation
preventing,
diagnosing,
treating
diseases
related
axis
from
perspective
cytokines,
EVs,
hormones,
metabolites.
Calcitonin
gene-related
peptide
(CGRP),
an
osteopromotive
neurotransmitter
with
a
short
half-life,
shows
increase
while
calcitonin
receptor-like
(CALCRL)
level
is
decreased
at
the
early
stage
in
bone
fractures.
Therefore,
activation
of
CALCRL-mediated
signaling
may
be
more
critical
to
promote
tendon-bone
healing.
We
found
CGRP
enhanced
osteogenic
differentiation
BMSCs
through
PKA/CREB/JUNB
pathway,
contributing
improved
sonic
hedgehog
(SHH)
expression,
which
was
verified
interface
(TBI)
mice
Calcrl
overexpression.
The
osteoblast-derived
SHH
and
slit
guidance
ligand
3
were
reported
favor
nerve
regeneration
type
H
(CD31
hi
EMCN
)
vessel
formation,
respectively.
Encouragingly,
or
inactivation
significantly
increased
intensity
fiber
TBI,
Simultaneously,
gait
characteristics
biomechanical
performance
observed
overexpression
group.
Together,
gene
therapy
targeting
receptor
therapeutic
strategy
sports
medicine.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Май 15, 2024
The
complexity
of
repairing
large
segment
defects
and
eradicating
residual
tumor
cell
puts
the
osteosarcoma
clinical
management
challenging.
Current
biomaterial
design
often
overlooks
crucial
role
precisely
regulating
innervation
in
bone
regeneration.
Here,
we
develop
a
Germanium
Selenium
(GeSe)
co-doped
polylactic
acid
(PLA)
nanofiber
membrane-coated
tricalcium
phosphate
bioceramic
scaffold
(TCP-PLA/GeSe)
that
mimics
bone-periosteum
structure.
This
biomimetic
offers
dual
functionality,
combining
piezoelectric
photothermal
conversion
capabilities
while
remaining
biodegradable.
When
subjected
to
ultrasound
irradiation,
US-electric
stimulation
TCP-PLA/GeSe
enables
spatiotemporal
control
neurogenic
differentiation.
feature
supports
early
during
formation,
promoting
differentiation
Schwann
cells
(SCs)
by
increasing
intracellular
Ca
