Crosstalk between Bone and Nerves within Bone

Advanced Science, Journal Year: 2021, Volume and Issue: 8(7)

Published: Feb. 10, 2021

Abstract For the past two decades, function of intrabony nerves on bone has been a subject intense research, while is still hidden in corner. In present review, possible crosstalk between and peripheral will be comprehensively analyzed. Peripheral participate development repair via host signals generated through secretion neurotransmitters, neuropeptides, axon guidance factors neurotrophins, with additional contribution from nerve‐resident cells. return, contributes to this microenvironmental rendezvous by housing within its internal milieu provide mechanical support protective shelf. A large ensemble chemical, mechanical, electrical cues works harmony marrow stromal cells regulation nerves. The not limited physiological state, but also involved various diseases including osteoporosis, osteoarthritis, heterotopic ossification, psychological stress‐related abnormalities, related tumors. This may harnessed design tissue engineering scaffolds for defects or targeted treatment

Language: Английский

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Osteoporosis pathogenesis and treatment: existing and emerging avenues

Cellular & Molecular Biology Letters, Journal Year: 2022, Volume and Issue: 27(1)

Published: Sept. 4, 2022

Abstract Osteoporotic fractures lead to increased disability and mortality in the elderly population. With rapid increase aging population around globe, more effective treatments for osteoporosis osteoporotic are urgently required. The underlying molecular mechanisms of believed be due activity osteoclasts, decreased osteoblasts, or both, which leads an imbalance bone remodeling process with accelerated resorption attenuated formation. Currently, available clinical have mostly focused on factors influencing remodeling; however, they their own limitations side effects. Recently, cytokine immunotherapy, gene therapy, stem cell therapy become new approaches treatment various diseases. This article reviews latest research mechanisms, as well how this underpins current potential novel osteoporosis.

Language: Английский

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Photosensitive and Conductive Hydrogel Induced Innerved Bone Regeneration for Infected Bone Defect Repair

Advanced Healthcare Materials, Journal Year: 2022, Volume and Issue: 12(3)

Published: Nov. 3, 2022

Repairing infected bone defects is a challenge in the field of orthopedics because limited self-healing capacity tissue and susceptibility refractory materials to bacterial activity. Innervation initiating factor for regeneration plays key regulatory role subsequent vascularization, ossification, mineralization processes. Infection leads necrosis local nerve fibers, impeding repair defects. Herein, biomaterial that can induce skeletal-associated neural network reconstruction with high antibacterial activity proposed treatment A photosensitive conductive hydrogel prepared by incorporating magnesium-modified black phosphorus (BP@Mg) into gelatin methacrylate (GelMA). The near-infrared irradiation-based photothermal photodynamic endows it strong activity, improving inflammatory microenvironment reducing bacteria-induced damage. nanosheets bioactive ions released from BP@Mg synergistically improve migration secretion Schwann cells, promote neurite outgrowth, facilitate innerved regeneration. In an skull defect model, GelMA-BP@Mg shows efficient promotes CGRP+ fiber phototherapy provides novel strategy based on innervation repair.

Language: Английский

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Neurovascular coupling in bone regeneration

Experimental & Molecular Medicine, Journal Year: 2022, Volume and Issue: 54(11), P. 1844 - 1849

Published: Nov. 29, 2022

Abstract The mammalian skeletal system is densely innervated by both neural and vascular networks. Peripheral nerves in the skeleton include sensory sympathetic nerves. crosstalk between tissues critical for development regeneration. cellular processes of osteogenesis angiogenesis are coupled physiological pathophysiological contexts. molecular regulation have yet to be fully defined. This review will provide a detailed characterization regulatory role blood vessels during bone Furthermore, given importance spatial relationship bone, we discuss neurovascular coupling pathological formation. A better understanding interactions inform future novel therapeutic targeting clinical repair

Language: Английский

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Polyhedron‐Like Biomaterials for Innervated and Vascularized Bone Regeneration

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(42)

Published: July 12, 2023

Neural-vascular networks are densely distributed through periosteum, cortical bone, and cancellous which is of great significance for bone regeneration remodeling. Although significant progress has been made in tissue engineering, ineffective regeneration, delayed osteointegration still remains an issue due to the ignorance intrabony nerves blood vessels. Herein, inspired by space-filling polyhedra with open architectures, polyhedron-like scaffolds spatial topologies prepared via 3D-printing technology mimic meshwork structure bone. Benefiting from its topologies, greatly promoted osteogenic differentiation mesenchymal stem cells (BMSCs) activating PI3K-Akt signals, exhibiting satisfactory performance on angiogenesis neurogenesis. Computational fluid dynamic (CFD) simulation elucidates that have a relatively lower area-weighted average static pressure, beneficial osteogenesis. Furthermore, vivo experiments further demonstrate obviously promote formation osteointegration, as well inducing vascularization ingrowth nerves, leading innervated vascularized regeneration. Taken together, this work offers promising approach fabricating multifunctional without additional exogenous seeding growth factors, holds potential functional clinical translation.

Language: Английский

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Neuro–bone tissue engineering: emerging mechanisms, potential strategies, and current challenges

Bone Research, Journal Year: 2023, Volume and Issue: 11(1)

Published: Dec. 20, 2023

The skeleton is a highly innervated organ in which nerve fibers interact with various skeletal cells. Peripheral endings release neurogenic factors and sense signals, mediate bone metabolism pain. In recent years, tissue engineering has increasingly focused on the effects of nervous system regeneration. Simultaneous regeneration nerves through use materials or by enhancement endogenous repair signals been proven to promote functional Additionally, emerging information mechanisms interoception central regulation homeostasis provide an opportunity for advancing biomaterials. However, comprehensive reviews this topic are lacking. Therefore, review provides overview relationship between regeneration, focusing applications. We discuss novel regulatory explore innovative approaches based nerve-bone interactions Finally, challenges future prospects field briefly discussed.

Language: Английский

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Nerve Growth Factor-Preconditioned Mesenchymal Stem Cell-Derived Exosome-Functionalized 3D-Printed Hierarchical Porous Scaffolds with Neuro-Promotive Properties for Enhancing Innervated Bone Regeneration

ACS Nano, Journal Year: 2024, Volume and Issue: 18(10), P. 7504 - 7520

Published: Feb. 27, 2024

The essential role of the neural network in enhancing bone regeneration has often been overlooked biomaterial design, leading to delayed or compromised healing. Engineered mesenchymal stem cells (MSCs)-derived exosomes are becoming increasingly recognized as potent cell-free agents for manipulating cellular behavior and improving therapeutic effectiveness. Herein, MSCs stimulated with nerve growth factor (NGF) regulate exosomal cargoes improve neuro-promotive potential facilitate innervated regeneration. In vitro cell experiments showed that NGF-stimulated MSCs-derived (N-Exos) obviously improved function neurotrophic effects cells, consequently, osteogenic osteo-reparative cells. Bioinformatic analysis by miRNA sequencing pathway enrichment revealed beneficial N-Exos may partly be ascribed NGF-elicited multicomponent miRNAs subsequent regulation activation MAPK PI3K-Akt signaling pathways. On this basis, were delivered on micropores 3D-printed hierarchical porous scaffold accomplish sustained release profile extended bioavailability. a rat model distal femoral defect, N-Exos-functionalized significantly induced neurovascular structure formation This study provided feasible strategy modulate functional acquire desirable potential. Furthermore, developed represent promising neurovascular-promotive reparative clinical translation.

Language: Английский

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Bone-organ axes: bidirectional crosstalk

Military Medical Research, Journal Year: 2024, Volume and Issue: 11(1)

Published: June 12, 2024

Abstract In addition to its recognized role in providing structural support, bone plays a crucial maintaining the functionality and balance of various organs by secreting specific cytokines (also known as osteokines). This reciprocal influence extends these modulating homeostasis development, although this aspect has yet be systematically reviewed. review aims elucidate bidirectional crosstalk, with particular focus on osteokines. Additionally, it presents unique compilation evidence highlighting critical function extracellular vesicles (EVs) within bone-organ axes for first time. Moreover, explores implications crosstalk designing implementing bone-on-chips assembloids, underscoring importance comprehending interactions advancing physiologically relevant vitro models. Consequently, establishes robust theoretical foundation preventing, diagnosing, treating diseases related axis from perspective cytokines, EVs, hormones, metabolites.

Language: Английский

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Activation of CGRP receptor–mediated signaling promotes tendon-bone healing

Science Advances, Journal Year: 2024, Volume and Issue: 10(10)

Published: March 8, 2024

Calcitonin gene-related peptide (CGRP), an osteopromotive neurotransmitter with a short half-life, shows increase while calcitonin receptor-like (CALCRL) level is decreased at the early stage in bone fractures. Therefore, activation of CALCRL-mediated signaling may be more critical to promote tendon-bone healing. We found CGRP enhanced osteogenic differentiation BMSCs through PKA/CREB/JUNB pathway, contributing improved sonic hedgehog (SHH) expression, which was verified interface (TBI) mice Calcrl overexpression. The osteoblast-derived SHH and slit guidance ligand 3 were reported favor nerve regeneration type H (CD31 hi EMCN ) vessel formation, respectively. Encouragingly, or inactivation significantly increased intensity fiber TBI, Simultaneously, gait characteristics biomechanical performance observed overexpression group. Together, gene therapy targeting receptor therapeutic strategy sports medicine.

Language: Английский

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Biomimetic bone-periosteum scaffold for spatiotemporal regulated innervated bone regeneration and therapy of osteosarcoma

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 15, 2024

The complexity of repairing large segment defects and eradicating residual tumor cell puts the osteosarcoma clinical management challenging. Current biomaterial design often overlooks crucial role precisely regulating innervation in bone regeneration. Here, we develop a Germanium Selenium (GeSe) co-doped polylactic acid (PLA) nanofiber membrane-coated tricalcium phosphate bioceramic scaffold (TCP-PLA/GeSe) that mimics bone-periosteum structure. This biomimetic offers dual functionality, combining piezoelectric photothermal conversion capabilities while remaining biodegradable. When subjected to ultrasound irradiation, US-electric stimulation TCP-PLA/GeSe enables spatiotemporal control neurogenic differentiation. feature supports early during formation, promoting differentiation Schwann cells (SCs) by increasing intracellular Ca

Language: Английский

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