Smart‐Responsive Multifunctional Therapeutic System for Improved Regenerative Microenvironment and Accelerated Bone Regeneration via Mild Photothermal Therapy

Advanced Science, Год журнала: 2023, Номер 11(2)

Опубликована: Ноя. 7, 2023

Abstract The treatment of bone defects remains a substantial clinical challenge due to the lack spatiotemporal management immune microenvironment, revascularization, and osteogenic differentiation. Herein, deferoxamine (DFO)‐loaded black phosphorus nanosheets decorated by polydopamine layer are prepared (BPPD) compounded into gelatin methacrylate/sodium alginate methacrylate (GA) hybrid hydrogel as smart‐responsive therapeutic system (GA/BPPD) for accelerated regeneration. BPPD nanocomposites served bioactive components near‐infrared (NIR) photothermal agents, which conferred with excellent NIR/pH dual‐responsive properties, realizing stimuli‐responsive release DFO PO 4 3 − during Under action NIR‐triggered mild therapy, GA/BPPD exhibited positive effect on promoting osteogenesis angiogenesis, eliminating excessive reactive oxygen species, inducing macrophage polarization M2 phenotype. More significantly, through polarization‐induced osteoimmune this platform could also drive functional cytokine secretion enhanced angiogenesis osteogenesis. In vivo experiments further demonstrated that facilitate healing attenuating local inflammatory response, increasing pro‐healing factors, stimulating endogenous cell recruitment, accelerating revascularization. Collectively, proposed intelligent provides promising strategy reshape damaged tissue microenvironment augmented

Язык: Английский

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Electroactive Biomaterials for Facilitating Bone Defect Repair under Pathological Conditions

Advanced Science, Год журнала: 2022, Номер 10(2)

Опубликована: Дек. 1, 2022

Bone degeneration associated with various diseases is increasing due to rapid aging, sedentary lifestyles, and unhealthy diets. Living bone tissue has bioelectric properties critical remodeling, under pathological conditions results in significant changes these properties. There growing interest utilizing biomimetic electroactive biomaterials that recapitulate the natural electrophysiological microenvironment of healthy promote repair. This review first summarizes etiology degenerative such as type II diabetes, osteoporosis, periodontitis, osteoarthritis, rheumatoid arthritis, osteomyelitis, metastatic osteolysis. Next, diverse array synthetic therapeutic potential are discussed. Putative mechanistic pathways by which can mitigate critically examined, including enhancement osteogenesis angiogenesis, suppression inflammation osteoclastogenesis, well their anti-bacterial effects. Finally, limited research on utilization treatment aforementioned examined. Previous studies have mostly focused using treat traumatic injuries. It hoped this will encourage more efforts use for treating conditions.

Язык: Английский

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Electroactive Biomaterials Regulate the Electrophysiological Microenvironment to Promote Bone and Cartilage Tissue Regeneration

Advanced Functional Materials, Год журнала: 2024, Номер 34(23)

Опубликована: Янв. 7, 2024

Abstract The incidence of large bone and articular cartilage defects caused by traumatic injury is increasing worldwide; the tissue regeneration process for these injuries lengthy due to limited self‐healing ability. Endogenous bioelectrical phenomenon has been well recognized play an important role in homeostasis regeneration. Studies have reported that electrical stimulation (ES) can effectively regulate various biological processes holds promise as external intervention enhance synthesis extracellular matrix, thereby accelerating Hence, electroactive biomaterials considered a biomimetic approach ensure functional recovery integrating physiological signals, including electrical, biochemical, mechanical signals. This review will discuss endogenous bioelectricity tissue, effects ES on cellular behaviors. Then, recent advances materials their applications are systematically overviewed, with focus advantages disadvantages repair performances modulation cell fate. Finally, significance mimicking electrophysiological microenvironment target emphasized future development challenges strategies proposed.

Язык: Английский

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Effects of Electric Field‐Modulated Conductive Hydrogel on Osseoperception and Osseointegration of Dental Implants

Advanced Functional Materials, Год журнала: 2024, Номер 34(28)

Опубликована: Март 14, 2024

Abstract Achieving optimal implant restoration hinges on both robust osseointegration as the structural foundation and favorable osseoperception for advanced masticatory function. The significance of nerve innervation in peri‐implant environment cornerstone is often underestimated. Despite integral role endogenous electric fields (EFs) human body, particularly electrosensitive tissues like tissue, current approach involving external electrical stimulation invasive not clinically applicable. present study introduces a conductive hydrogel designed to respond EFs, aiming foster regeneration around dental implants coordinate osseoperception. promotes neurite outgrowth by upregulating intracellular Ca 2+ concentration activating subsequent pathways. Furthermore, enhanced release neuropeptides from neurocells improves osteogenesis osteoblasts. impact also thoroughly investigated vivo. This represents unique strategy enhancing within EFs environment. advancement opens door achieving physiological psychological integration implants.

Язык: Английский

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Injectable ultrasound-powered bone-adhesive nanocomposite hydrogel for electrically accelerated irregular bone defect healing

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Фев. 7, 2024

The treatment of critical-size bone defects with irregular shapes remains a major challenge in the field orthopedics. Bone implants adaptability to complex morphological defects, bone-adhesive properties, and potent osteogenic capacity are necessary. Here, shape-adaptive, highly bone-adhesive, ultrasound-powered injectable nanocomposite hydrogel is developed via dynamic covalent crosslinking amine-modified piezoelectric nanoparticles biopolymer networks for electrically accelerated healing. Depending on inorganic-organic interaction between amino-modified bio-adhesive network, adhesive strength prepared exhibited an approximately 3-fold increase. In response ultrasound radiation, could generate controllable electrical output (-41.16 61.82 mV) enhance effect vitro vivo significantly. Rat calvarial defect repair validates addition, bioinformatics analysis reveals that ultrasound-responsive enhanced differentiation mesenchymal stem cells by increasing calcium ion influx up-regulating PI3K/AKT MEK/ERK signaling pathways. Overall, present work novel wireless broadens therapeutic horizons defects.

Язык: Английский

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Bioinspired soft-hard combined system with mild photothermal therapeutic activity promotes diabetic bone defect healing via synergetic effects of immune activation and angiogenesis

Theranostics, Год журнала: 2024, Номер 14(10), С. 4014 - 4057

Опубликована: Янв. 1, 2024

Background:The comprehensive management of diabetic bone defects remains a substantial clinical challenge due to the hostile regenerative microenvironment characterized by aggravated inflammation, excessive reactive oxygen species (ROS), bacterial infection, impaired angiogenesis, and unbalanced homeostasis.Thus, an advanced multifunctional therapeutic platform capable simultaneously achieving immune regulation, elimination, tissue regeneration is urgently designed for augmented under pathological milieu.Methods Results: Herein, photoactivated soft-hard combined scaffold system (PGCZ) was engineered introducing polydopamine-modified zeolitic imidazolate framework-8-loaded double-network hydrogel (soft matrix component) into 3D-printed poly(ε-caprolactone) (PCL) (hard component).The versatile PGCZ based on PCL thus prepared features highly extracellular matrix-mimicking microstructure, suitable biodegradability mechanical properties, excellent photothermal performance, allowing long-term structural stability support regeneration.Under periodic near-infrared (NIR) irradiation, localized effect triggers on-demand release Zn 2+ , which, together with repeated mild hyperthermia, collectively accelerates proliferation osteogenic differentiation preosteoblasts potently inhibits growth biofilm formation.Additionally, also presents outstanding immunomodulatory ROS scavenging capacities, which regulate M2 polarization macrophages drive functional cytokine secretion, leading pro-regenerative in situ enhanced vascularization.In vivo experiments further demonstrated that conjunction activity remarkably attenuated local inflammatory cascade, initiated endogenous stem cell recruitment neovascularization, orchestrated osteoblast/osteoclast balance, ultimately accelerating regeneration. Ivyspring

Язык: Английский

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Selenoprotein‐Regulated Hydrogel for Ultrasound‐Controlled Microenvironment Remodeling to Promote Bone Defect Repair

Advanced Functional Materials, Год журнала: 2024, Номер 34(12)

Опубликована: Янв. 14, 2024

Abstract Abnormal levels of reactive oxygen species (ROS) and the hypoxic microenvironment within bone defects are important factors that impede repair processes. Herein, an innovative ultrasound‐modulatable hydrogel platform with selenoprotein‐mediated antioxidant effects to promote injury is presented. This encapsulates oxygen‐enriched selene‐incorporated thin‐shell silicon methacrylate gelatin (O 2 ‐PSSG). The resultant construct orchestrates modulation bone‐defect microenvironment, thereby expediting course regeneration. Ultrasound (US) used regulate pore size release selenium‐containing nanoparticles in situ synthesis efficient intracellular selenoproteins hydrogen peroxide consumption. As expected, O ‐PSSG rapidly releases selenocystine ([Sec] ) under US control scavenge maintain homeostasis marrow mesenchymal stem cells (BMSCs). Over time, action system by selenoprotein increases activation Wnt/β‐catenin pathways promotes differentiation BMSCs. Consequently, potentiates proficiency BMSCs both vitro vivo, alleviates environments, osteogenic differentiation, expedites cranial rat models. In summary, this study suggests designed constructed US‐responsive a promising prospective strategy for addressing fostering

Язык: Английский

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Biomaterials for diabetic bone repair: Influencing mechanisms, multi-aspect progress and future prospects

Composites Part B Engineering, Год журнала: 2024, Номер 274, С. 111282 - 111282

Опубликована: Фев. 5, 2024

Язык: Английский

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Advances in electroactive biomaterials: Through the lens of electrical stimulation promoting bone regeneration strategy

Journal of Orthopaedic Translation, Год журнала: 2024, Номер 47, С. 191 - 206

Опубликована: Июнь 27, 2024

The regenerative capacity of bone is indispensable for growth, given that accidental injury almost inevitable. Bone relevant the aging population globally and repair large defects after osteotomy (e.g., following removal malignant tumours). Among many therapeutic modalities proposed to regeneration, electrical stimulation has attracted significant attention owing its economic convenience exceptional curative effects, various electroactive biomaterials have emerged. This review summarizes current knowledge progress regarding strategies improving repair. Such range from traditional methods delivering via electroconductive materials using external power sources self-powered biomaterials, such as piezoelectric nanogenerators. Electrical osteogenesis are related piezoelectricity. examines cell behaviour potential mechanisms electrostimulation in healing, aiming provide new insights regeneration biomaterials. roles rehabilitating microenvironment facilitate addressing whereby cues mediate regeneration. Interactions between osteogenesis-related cells summarized, leading proposals use stimulation-based therapies accelerate healing.

Язык: Английский

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Manipulation of Surface Electrical Charge on Nanocomposite Membranes Confers Wide Spectrum Bactericidal Effects and Promotes Tissue Regeneration

Advanced Functional Materials, Год журнала: 2024, Номер 34(22)

Опубликована: Фев. 5, 2024

Abstract Utilization of electro‐responsive biomaterials with antibacterial properties is advantageous for facilitating septic wound healing and tissue regeneration. However, the dose‐response effects electrical stimuli from these materials against bacteria are not rigorously characterized, achieving synergy bactericidal pro‐regenerative remains a major challenge. Here, graded series flexible BaTiO 3 /P(VDF‐TrFE) electroactive nanocomposite membranes (EMs) developed varying surface charge intensities, to serve as dressing healing. EMs display broad‐spectrum both Gram‐positive Gram‐negative in dose‐dependent manner, depending on magnitude their potential. Mechanistically, increase intracellular levels reactive oxygen species within cells, which turn caused oxidative damage bacterial membrane, thereby suppressing activity biofilm formation. Moreover, vivo studies demonstrated that effectively inhibited S. aureus infection accelerated mouse skin defect model, well ameliorated P. gingivalis‐ mediated periodontal inflammation periodontitis model. Hence, this study optimizes characterizes bacteria, thus validating therapeutic applications combating infection.

Язык: Английский

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