Fluorescent Probes for Disease Diagnosis

Chemical Reviews, Год журнала: 2024, Номер 124(11), С. 7106 - 7164

Опубликована: Май 17, 2024

The identification and detection of disease-related biomarkers is essential for early clinical diagnosis, evaluating disease progression, the development therapeutics. Possessing advantages high sensitivity selectivity, fluorescent probes have become effective tools monitoring active molecules at cellular level in vivo. In this review, we describe current designed quantification key bioactive associated with common diseases, such as organ damage, inflammation, cancers, cardiovascular brain disorders. We emphasize strategies behind design capable biomarker diagnosis cover some aspects combined diagnostic/therapeutic based on regulating molecules. This review concludes a discussion challenges outlook probes, highlighting future avenues research that should enable these to achieve accurate biomedical applications.

Язык: Английский

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Rational Design of a Double-Locked Photoacoustic Probe for Precise In Vivo Imaging of Cathepsin B in Atherosclerotic Plaques

Journal of the American Chemical Society, Год журнала: 2023, Номер 145(32), С. 17881 - 17891

Опубликована: Авг. 2, 2023

Atherosclerotic plaque rupture is a significant cause of acute cardiovascular events such as heart attack and stroke, triggered by the decomposition fiber caps induced cysteine cathepsin. However, accurate measurement cathepsin B (CTB) activity in plaques challenging due to low specificity insufficient penetration depth available atherosclerosis-associated fluorescent probes, hampering reliable assessment vulnerability. To address these limitations, we added both lipophilic alkyl chain hydrophilic CTB substrate hemicyanine scaffold develop lipid-unlocked responsive probe (L-CRP) that uses lipids two keys unlock photoacoustic (PA) signals for measuring environments. Such properties allow L-CRP imaging specific activities foam cells atherosclerotic while keeping silence toward lipid-deficient environments, M1-type macrophages LPS-induced inflammatory lesions. Moreover, activatable PA exhibit deeper tissue ability (>1.0 cm) than current probes based on near-infrared (∼0.3 cm), suitable atherosclerosis living mice. In mice, dynamically reports intraplaque levels, which well-correlated with vulnerability characteristics cap thickness, macrophage recruitment, necrotic core size, thus enabling risk stratification mice complicated pneumonia. successfully identifies excised human artery tissues, promising auxiliary diagnosis clinical application.

Язык: Английский

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An AND Logic Gate for Magnetic‐Resonance‐Imaging‐Guided Ferroptosis Therapy of Tumors

Advanced Materials, Год журнала: 2023, Номер 35(45)

Опубликована: Сен. 17, 2023

To improve the magnetic resonance imaging (MRI) efficiency and ferroptosis therapy efficacy of exceedingly small iron oxide nanoparticles (IO, <5 nm) for tumors via enhancing sensitivity tumor microenvironment (TME) responsiveness, inspired by molecular logic gates, a self-assembled IO with an AND gate function is designed constructed. Typically, cystamine (CA) conjugated onto end poly(2-methylthio-ethanol methacrylate) (PMEMA) to generate PMEMA-CA. The PMEMA-CA grafted surface brequinar (BQR)-loaded form IO-BQR@PMEMA. IO-BQR@PMEMA (SA-IO-BQR@PMEMA) obtained due hydrophobicity PMEMA. carbon-sulfur single bond can be oxidized reactive oxygen species (ROS) in TME thio-oxygen double bond, resulting conversion from being hydrophobic hydrophilic. disulfide broken glutathione (GSH) TME, leading shedding PMEMA surface. Under dual actions ROS GSH (i.e., gate), SA-IO-BQR@PMEMA disassembled release IO, Fe2+/3+ , BQR. In vitro vivo results demonstrate mechanism, high T1 MRI performance exceptional tumors, excellent biosafety SA-IO-BQR@PMEMA.

Язык: Английский

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An “AND” Molecular Logic Gate as a Super‐Enhancers for De Novo Designing Activatable Probe and Its Application in Atherosclerosis Imaging

Advanced Science, Год журнала: 2023, Номер 10(12)

Опубликована: Фев. 19, 2023

Developing activatable fluorescent probes with superlative fluorescence enhancement factor (F/F0 ) to improve the signal-to-noise (S/N) ratio is still an urgent issue. "AND" molecular logic gates are emerging as a useful tool for enhanced selectivity and accuracy. Here, gate developed super-enhancers designing huge F/F0 S/N ratio. It utilizes lipid-droplets (LDs) controllable background input sets target analyte variable input. The tremendously quenching due double locking, thus extreme of obtained. Importantly, this probe can transfer LDs after response occurs. be directly visualized through spatial location without control group. Accordingly, peroxynitrite (ONOO- (CNP2-B) de novo designed. CNP2-B achieves 2600 reacting ONOO- . Furthermore, from mitochondria lipid droplets being activated. higher obtained than commercial 3'-(p-hydroxyphenyl) fluorescein (HPFin vitro in vivo. Therefore, atherosclerotic plaques at mouse models delineated clearly administration situ gel. Such envisioned execute more imaging tasks.

Язык: Английский

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Screening drug-induced liver injury through two independent parameters of lipid droplets and peroxynitrite with a π-extended coumarin-based NIR fluorescent probe

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 410, С. 135659 - 135659

Опубликована: Март 19, 2024

Язык: Английский

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Novel BODIPY-based NIR fluorescent probe with appropriate Stokes shift for diagnosis and treatment evaluation of epilepsy via imaging ONOO− fluctuation

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 423, С. 136766 - 136766

Опубликована: Окт. 11, 2024

Язык: Английский

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Ultrasensitive Optical Detection and Elimination of Residual Microtumors with a Postoperative Implantable Hydrogel Sensor for Preventing Cancer Recurrence

Advanced Materials, Год журнала: 2024, Номер 36(14)

Опубликована: Янв. 4, 2024

Abstract In vivo optical imaging of trace biomarkers in residual microtumors holds significant promise for cancer prognosis but poses a formidable challenge. Here, novel hydrogel sensor is designed ultrasensitive and specific the elusive biomarker. This seamlessly integrates molecular beacon nanoprobe with fibroblasts, offering both high tissue retention capability an impressive signal‐to‐noise ratio imaging. Signal amplification accomplished through exonuclease I‐mediated biomarker recycling. The resulting sensitively detects carcinoembryonic antigen detection limit 1.8 pg mL −1 test tubes. Moreover, it successfully identifies nodules median diameter less than 2 mm mice bearing partially removed primary triple‐negative breast carcinomas (4T1). Notably, this proven effective sensitive diagnosis invasive tumors post‐surgical infiltrating 4T1 cells, leveraging role fibroblasts locally enriching tumor cells. Furthermore, microtumor rapidly photothermal ablation by polydopamine‐based under guidance visualization, achieving ≈100% suppression recurrence lung metastasis. work offers promising alternative strategy visually detecting microtumors, potentially enhancing patients following surgical interventions.

Язык: Английский

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“Zero” Intrinsic Fluorescence Sensing‐Platforms Enable Ultrasensitive Whole Blood Diagnosis and In Vivo Imaging

Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(16)

Опубликована: Фев. 27, 2024

Abnormal physiological processes and diseases can lead to content or activity fluctuations of biocomponents in organelles whole blood. However, precise monitoring these abnormalities remains extremely challenging due the insufficient sensitivity accuracy available fluorescence probes, which be attributed background arising from two sources, 1) biocomponent autofluorescence (BCAF) 2) probe intrinsic (PIF). To overcome obstacles, we have re-engineered far-red NIR II rhodol derivatives that possess weak BCAF interference. And a series "zero" PIF sensing-platforms were created by systematically regulating open-loop/spirocyclic forms. Leveraging advancements, devised various ultra-sensitive indicators, achieving substantial boosts (190 1300-fold). Among 8-LAP demonstrated accurate tracking quantifying leucine aminopeptidase (LAP) blood at stages tumor metastasis. Furthermore, coupling with an endoplasmic reticulum-targeting element enabled detection ERAP1 HCT116 cells p53 abnormalities. This delicate design eliminating provides insights into enhancing existing probes toward imaging abnormal diseases.

Язык: Английский

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Molecular Engineering of a Doubly Quenched Fluorescent Probe Enables Ultrasensitive Detection of Biothiols in Highly Diluted Plasma and High-Fidelity Imaging of Dihydroartemisinin-Induced Ferroptosis

Analytical Chemistry, Год журнала: 2024, Номер 96(32), С. 13260 - 13269

Опубликована: Авг. 1, 2024

The occurrence and development of diseases are accompanied by abnormal activity or concentration biomarkers in cells, tissues, blood. However, the insufficient sensitivity accuracy available fluorescence probes hinder precise monitoring associated indexes biological systems, which is generally due to high probe intrinsic false-negative signal caused reactive oxygen species (ROS)-induced decomposition. To resolve these problems, we have engineered a ROS-stable, meso-carboxylate boron dipyrromethene (BODIPY)-based fluorescent probe, displays quite low background doubly quenched combined strategy photoinduced electron transfer (PET) effect "ester-to-carboxylate" conversion. achieved S/N ratio with ultrasensitivity good selectivity toward biothiols, endowing its fast detection capability biothiol level 200×-diluted plasma samples. Using this remarkable distinguishing liver injury from normal even at 80× dilution. Moreover, owing stability ROS, was successfully employed for high-fidelity imaging negative fluctuation nonsmall-cell lung cancer (NSCLC) during dihydroartemisinin-induced ferroptosis. This delicate design suppressing reveals insights into enhancing diseases.

Язык: Английский

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A novel dual-channel fluorescent probe for the detection of peroxynitrite anions and lipid droplets in epileptic disease

Analytica Chimica Acta, Год журнала: 2025, Номер 1350, С. 343863 - 343863

Опубликована: Фев. 24, 2025

Язык: Английский

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