Molecular Engineering of a Doubly Quenched Fluorescent Probe Enables Ultrasensitive Detection of Biothiols in Highly Diluted Plasma and High-Fidelity Imaging of Dihydroartemisinin-Induced Ferroptosis

Analytical Chemistry, Год журнала: 2024, Номер 96(32), С. 13260 - 13269

Опубликована: Авг. 1, 2024

The occurrence and development of diseases are accompanied by abnormal activity or concentration biomarkers in cells, tissues, blood. However, the insufficient sensitivity accuracy available fluorescence probes hinder precise monitoring associated indexes biological systems, which is generally due to high probe intrinsic false-negative signal caused reactive oxygen species (ROS)-induced decomposition. To resolve these problems, we have engineered a ROS-stable, meso-carboxylate boron dipyrromethene (BODIPY)-based fluorescent probe, displays quite low background doubly quenched combined strategy photoinduced electron transfer (PET) effect "ester-to-carboxylate" conversion. achieved S/N ratio with ultrasensitivity good selectivity toward biothiols, endowing its fast detection capability biothiol level 200×-diluted plasma samples. Using this remarkable distinguishing liver injury from normal even at 80× dilution. Moreover, owing stability ROS, was successfully employed for high-fidelity imaging negative fluctuation nonsmall-cell lung cancer (NSCLC) during dihydroartemisinin-induced ferroptosis. This delicate design suppressing reveals insights into enhancing diseases.

Язык: Английский

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Advances in secondary prevention mechanisms of macrovascular complications in type 2 diabetes mellitus patients: a comprehensive review

European journal of medical research, Год журнала: 2024, Номер 29(1)

Опубликована: Март 4, 2024

Type 2 diabetes mellitus (T2DM) poses a significant global health burden. This is particularly due to its macrovascular complications, such as coronary artery disease, peripheral vascular and cerebrovascular which have emerged leading contributors morbidity mortality. review comprehensively explores the pathophysiological mechanisms underlying these protective strategies, both existing emerging secondary preventive measures. Furthermore, we delve into applications of experimental models methodologies in foundational research while also highlighting current limitations future directions. Specifically, focus on literature published post-2020 concerning prevention complications patients with T2DM by conducting targeted studies supported robust evidence offer holistic perspective.

Язык: Английский

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Advancements in molecular disassembly of optical probes: a paradigm shift in sensing, bioimaging, and therapeutics

Nanoscale Horizons, Год журнала: 2024, Номер 9(9), С. 1390 - 1416

Опубликована: Янв. 1, 2024

Molecular disassembly is pioneering a new route to refined diagnostic and therapeutic solutions. This approach breaks down self-assembled molecules, offering enhanced precision efficiency in various bio-oriented applications.

Язык: Английский

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A cysteine/lipid droplets sequentially activated dual-locked fluorescent probe for accurate bioimaging of tumor tissues

Dyes and Pigments, Год журнала: 2024, Номер 224, С. 112001 - 112001

Опубликована: Янв. 30, 2024

Язык: Английский

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Progress and Perspective of Atherosclerosis-Targeted Molecular Probes: From Precise Imaging to Auxiliary Diagnosis and Treatment

Coordination Chemistry Reviews, Год журнала: 2024, Номер 510, С. 215850 - 215850

Опубликована: Апрель 8, 2024

Язык: Английский

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Reactive Oxygen Species-Responsive Sequentially Targeted AIE Fluorescent Probe for Precisely Identifying the Atherosclerotic Plaques

ACS Applied Materials & Interfaces, Год журнала: 2023, Номер 15(40), С. 47381 - 47393

Опубликована: Сен. 28, 2023

The formation of atherosclerosis is the root cause various cardiovascular diseases (CVDs). Therefore, effective CVD interventions call for precise identification plaques to aid in clinical treatment such diseases. Herein, a reactive oxygen species (ROS)-responsive sequentially targeted fluorescent probe developed atherosclerotic plaque recognition. An aggregation-induced emission active fluorophore linked maleimide (polyethylene glycol) hydroxyl with ROS-responsive cleavable bond, which further functionalized CLIKKPF peptide (TPAMCF) specifically binding phosphatidylserine foam cells. After being assembled aqueous medium, TPAMCF nanoparticles can efficiently accumulate through high affinity externalized Activated by locally accumulated ROS cells, are interrupted, and then TPA be released subsequently identify lipid droplets inside cells achieve fluorescence imaging plaques. Such nanoprobes have favorable response performance exhibit special target vitro. In addition, nanoprobe-based permitted high-contrast detection specimens ex vivo. as promising probe, capable potential candidate plaque.

Язык: Английский

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Ionic-confining-assisted multiple-mode tunable light emitting of carbon nanodots

Chemical Engineering Journal, Год журнала: 2024, Номер 486, С. 150196 - 150196

Опубликована: Март 5, 2024

Язык: Английский

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Trident anchoring-lipid droplets strategy for fluorescence/super-resolution/lifetime imaging and diagnosis of fatty liver in vivo

Chemical Engineering Journal, Год журнала: 2024, Номер 490, С. 151810 - 151810

Опубликована: Апрель 30, 2024

Язык: Английский

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Activatable chemiluminescence probe based on four-arm PEG-conjugated-pyropheophorbide-a for in vivo autofluorescence-free imaging of peroxynitrite

Science China Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 6, 2025

Язык: Английский

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A novel ultra-sensitive fluorescent probe for monitoring and imaging drug-induced/inhibited ferroptosis-mediated liver injury model via peroxynitrite fluctuations

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 112833 - 112833

Опубликована: Янв. 1, 2025

Язык: Английский

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