Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 16, 2024
Abstract
Ischemia‐reperfusion
injury
(IRI)
is
the
leading
cause
of
hepatic
graft
dysfunction,
resulting
from
hepatocyte
damage.
Nevertheless,
given
few
specialized
therapeutics
available
in
IRI,
additional
mechanistic
insights
into
damage
are
required.
Here,
protein
solute
carrier
family
39
member
14
(SLC39A14)
identified
as
a
pro‐ferroptosis
target
hepatocytes
human
liver
allografts
through
single‐cell
RNA
sequencing
analysis.
SLC39A14
knockdown
significantly
mitigated
IRI
by
preventing
ferroptosis
vivo
and
vitro.
Mechanistically,
inhibition
suppressed
non‐transferrin‐bound
iron
(NTBI)
uptake
hepatocytes,
thereby
reducing
overload
cell
ferroptosis.
Moreover,
bone
marrow‐derived
mesenchymal
stem
cells
(hBMSCs)
found
to
exhibit
notable
therapeutic
effect
on
downregulating
expression.
Exosomes
derived
hBMSCs
delivered
abundant
miR‐16‐5p
which
post‐transcriptionally
expression
reduced
induced
IRI.
In
conclusion,
triggers
mediating
NTBI
inducing
hBMSC‐based
therapy
promising
reverse
this
progression
Advanced Materials,
Год журнала:
2024,
Номер
36(32)
Опубликована: Июнь 1, 2024
Abstract
Burn
wounds
often
bring
high
risks
of
delayed
healing
process
and
even
death.
Reactive
oxygen
species
(ROS)
play
a
crucial
role
in
burn
wound
repair.
However,
the
dynamic
requires
both
generation
ROS
to
inhibit
bacteria
subsequent
reduction
levels
initiate
promote
tissue
regeneration,
which
calls
for
more
intelligent
regulation
dressing
system.
Hence,
dual‐layered
hydrogel
(Dual‐Gel)
tailored
repair
is
designed:
inner
layer
(Gel
2)
first
responds
bacterial
hyaluronidase
(Hyal)
deliver
aggregation‐induced
emission
photosensitizer
functionalized
adipose‐derived
stem
cell
nanovesicles,
generate
upon
light
irradiation
eliminate
bacteria;
then
outer
1)
continuously
starts
long‐lasting
consumption
excess
at
site
accelerate
regeneration.
Simultaneously,
nanovesicles
trapped
burns
also
provide
nutrients
mobilize
neighboring
tissues
thoroughly
assist
inflammation
regulation,
proliferation,
migration,
angiogenesis.
In
summary,
this
study
develops
an
treatment
approach
on
by
programmatically
regulating
facilitating
comprehensive
Advanced Materials,
Год журнала:
2024,
Номер
36(33)
Опубликована: Июнь 27, 2024
Extracellular
vesicles
(EVs)
are
promising
next-generation
therapeutics
and
drug
delivery
systems
due
to
demonstrated
safety
efficacy
in
preclinical
models
early-stage
clinical
trials.
There
is
an
urgent
need
address
the
immunogenicity
of
EVs
(beyond
apparent
lack
immunotoxicity)
advance
development.
To
date,
few
studies
have
assessed
unintended
immunological
recognition
EVs.
An
in-depth
understanding
EV-induced
clearance
necessary
develop
effective
therapeutic
strategies,
including
approaches
mitigate
when
undesired.
This
article
summarizes
various
factors
involved
potential
strategies
reduce
for
improved
benefit.
ACS Nano,
Год журнала:
2024,
Номер
18(32), С. 21091 - 21111
Опубликована: Авг. 5, 2024
Milk
exosomes
(mExos)
have
demonstrated
significant
promise
as
vehicles
for
the
oral
administration
of
protein
and
peptide
drugs
owing
to
their
superior
capacity
traverse
epithelial
barriers.
Nevertheless,
certain
challenges
persist
due
intrinsic
characteristics,
including
suboptimal
drug
loading
efficiency,
inadequate
mucus
penetration
capability,
susceptibility
membrane
loss.
Herein,
a
hybrid
vesicle
with
self-adaptive
surface
properties
(mExos@DSPE-Hyd-PMPC)
was
designed
by
fusing
functionalized
liposomes
natural
mExos,
aiming
overcome
limitations
associated
mExos
unlock
full
potential
in
delivery.
The
property
transformation
mExos@DSPE-Hyd-PMPC
achieved
introducing
pH-sensitive
hydrazone
bond
between
highly
hydrophilic
zwitterionic
polymer
phospholipids,
utilizing
pH
microenvironment
on
jejunum
surface.
In
comparison
vesicles
exhibited
2.4-fold
enhancement
encapsulation
efficiency
semaglutide
(SET).
neutrally
charged
surfaces
jejunal
lumen
improved
preservation
proteins
efficient
traversal
barrier.
Upon
reaching
cells,
retained
positively
efficiently
overcame
apical
barrier,
intracellular
transport
basolateral
exocytosis
resulted
an
bioavailability
8.7%
notably
enhanced
pharmacological
therapeutic
effects.
This
study
successfully
addresses
some
holds
overcoming
sequential
absorption
barriers
delivery
peptides.
Extracellular
vesicles
(EVs),
involved
in
essential
physiological
and
pathological
processes
of
the
organism,
have
emerged
as
powerful
tools
for
disease
treatment
owing
to
their
unique
natural
biological
characteristics
artificially
acquired
advantages.
However,
limited
targeting
ability,
insufficient
production
yield,
low
drug-loading
capability
simplex
EVs
greatly
hindered
development
clinical
translation.
Therefore,
establishment
multifunctional
hybrid
membrane
nanovesicles
(HMNVs)
with
favorable
adaptability
flexibility
has
become
key
expanding
practical
application
EVs.
This
timely
review
summarizes
current
progress
HMNVs
biomedical
applications.
Different
preparation
strategies
including
physical,
chemical,
chimera
approaches
are
first
discussed.
then
individually
describes
diverse
types
based
on
homologous
or
heterologous
cell
substances,
a
fusion
liposome,
well
bacterial
membrane.
Subsequently,
specific
emphasis
is
placed
highlight
applications
toward
various
diseases
representative
examples.
Finally,
ongoing
challenges
prospects
currently
developed
translational
briefly
presented.
will
not
only
stimulate
broad
interest
among
researchers
from
disciplines
but
also
provide
valuable
insights
promising
nanoplatforms
precision
medicine.
Journal of Hematology & Oncology,
Год журнала:
2024,
Номер
17(1)
Опубликована: Июль 19, 2024
Abstract
Immunotherapy
has
become
an
important
part
of
the
oncotherapy
arsenal.
Its
applicability
in
various
cancer
types
is
impressive,
as
well
its
use
endogenous
mechanisms
to
achieve
desired
ends.
However,
off-target
or
on-target-off-tumor
toxicity,
limited
activity,
lack
control
combination
treatments
and,
especially
for
solid
tumors,
low
local
accumulation,
have
collectively
clinical
thereof.
These
limitations
are
partially
alleviated
by
delivery
systems.
Lipid-based
nanoparticles
(NPs)
emerged
revolutionary
carriers
due
favorable
physicochemical
characteristics,
with
specific
applications
and
strengths
particularly
useful
immunotherapeutic
agent
delivery.
The
aim
this
review
highlight
challenges
faced
immunotherapy
how
lipid-based
NPs
been,
may
be
further
utilized
address
such
challenges.
We
discuss
recent
fundamental
a
range
areas
provide
detailed
discussion
main
obstacles
immune
checkpoint
inhibition
therapies,
adoptive
cellular
cytokine
therapies.
nanosystems
could
these
through
either
delivery,
direct
modulation
system,
targeting
immunosuppressive
tumor
microenvironment.
explore
advanced
emerging
liposomal
lipid
nanoparticle
(LNP)
systems
nucleic
acid
intrinsic
extrinsic
stimulus-responsive
formulations,
biomimetic
immunotherapy.
Finally,
we
key
relating
NP
immunotherapies,
suggesting
future
research
directions
near
term
realize
potential
innovative
nanosystems,
they
crucial
steppingstone
towards
necessary
enhancement
efficacy
Graphical
abstract
Abstract
Extracellular
vesicles
(EVs),
crucial
mediators
of
cell‐to‐cell
communication,
hold
significant
diagnostic
potential
due
to
their
ability
concentrate
protein
biomarkers
in
bodily
fluids.
However,
challenges
isolating
EVs
from
biological
specimens
hinder
widespread
use.
The
preferred
strategy
involves
direct
analysis,
integrating
isolation
and
analysis
solutions,
with
immunoaffinity
methods
currently
dominating.
Yet,
the
heterogeneous
nature
poses
challenges,
as
proposed
markers
may
not
be
universally
present
thought,
raising
concerns
about
biomarker
screening
reliability.
This
issue
extends
EV‐mimics,
where
conventional
lack
applicability.
Addressing
these
study
reports
on
Membrane
Sensing
Peptides
(MSP)
pan‐vesicular
affinity
ligands
for
both
non‐canonical
analogs,
streamlining
capture
phenotyping
through
Single
Molecule
Array
(SiMoA).
MSP
enable
circulating
EVs,
eliminating
need
prior
isolation.
Demonstrating
clinical
translation,
technology
detects
an
EV‐associated
epitope
signature
serum
plasma,
distinguishing
myocardial
infarction
stable
angina.
Additionally,
allow
tetraspanin‐lacking
Red
Blood
Cell‐derived
overcoming
limitations
associated
antibody‐based
methods.
Overall,
work
underlines
value
complementary
tools
antibodies,
advancing
EV
diagnostics
beyond,
marking
first‐ever
peptide‐based
application
SiMoA
technology.
Abstract
Diabetic
foot
ulcer
(DFU),
characterized
by
high
recurrence
rate,
amputations
and
mortality,
poses
a
significant
challenge
in
diabetes
management.
The
complex
pathology
involves
dysregulated
glucose
homeostasis
leading
to
systemic
local
microenvironmental
complications,
including
peripheral
neuropathy,
micro-
macro-angiopathy,
recurrent
infection,
persistent
inflammation
re-epithelialization.
Novel
approaches
accelerate
DFU
healing
are
actively
pursued,
with
focus
on
utilizing
exosomes.
Exosomes
natural
nanovesicles
mediating
cellular
communication
containing
diverse
functional
molecular
cargos,
DNA,
mRNA,
microRNA
(miRNA),
lncRNA,
proteins,
lipids
metabolites.
While
some
exosomes
show
promise
modulating
function
promoting
healing,
their
efficacy
is
limited
low
yield,
impurities,
loading
content
inadequate
targeting.
Engineering
enhance
curative
activity
represents
potentially
more
efficient
approach
for
DFUs.
This
could
facilitate
focused
repair
regeneration
of
nerves,
blood
vessels
soft
tissue
after
development.
review
provides
an
overview
pathogenesis,
strategies
exosome
engineering
the
targeted
therapeutic
application
engineered
addressing
critical
pathological
changes
associated
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Фев. 22, 2024
Gastric
cancer
(GC)
is
a
malignant
neoplasm
originating
from
the
epithelial
cells
of
gastric
mucosa.
The
pathogenesis
GC
intricately
linked
to
tumor
microenvironment
within
which
reside.
Tumor-associated
macrophages
(TAMs)
primarily
differentiate
peripheral
blood
monocytes
and
can
be
broadly
categorized
into
M1
M2
subtypes.
M2-type
TAMs
have
been
shown
promote
growth,
tissue
remodeling,
angiogenesis.
Furthermore,
they
actively
suppress
acquired
immunity,
leading
poorer
prognosis
reduced
tolerance
chemotherapy.
Exosomes,
contain
myriad
biologically
active
molecules
including
lipids,
proteins,
mRNA,
noncoding
RNAs,
emerged
as
key
mediators
communication
between
TAMs.
exchange
these
via
exosomes
markedly
influence
consequently
impact
progression.
Recent
studies
elucidated
correlation
various
clinicopathological
parameters
GC,
such
size,
differentiation,
infiltration
depth,
lymph
node
metastasis,
TNM
staging,
highlighting
pivotal
role
in
development
metastasis.
In
this
review,
we
aim
comprehensively
examine
bidirectional
TAMs,
implications
alterations
on
immune
escape,
invasion,
metastasis
targeted
therapeutic
approaches
for
efficacy
potential
drug
resistance
strategies.
